LOCUS       sequence-386027-         291 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial production of soluble, active target proteins; Nterm 
            thrombin and enterokinase cleavage sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Ferreira AS, Lopacinski A, Batista M, Hiraiwa PM, Guimaraes BG, 
            Zanchin NIT
  TITLE     A toolkit for recombinant production of seven human EGF family 
            growth factors in active conformation.
  JOURNAL   Sci Rep. 2022 Mar 23;12(1):5034. doi: 10.1038/s41598-022-09060-9.
  PUBMED    35322149
REFERENCE   2  (bases 1 to 291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-03-23"; volume: "12(1):5034. doi"; pages: " 
            10.1038/s41598-022-09060-9"
COMMENT     Sequence Label: pET32a-hAREG
FEATURES             Location/Qualifiers
     source          1..291
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agtgtgcgtg tggaacaggt ggttaaaccg ccgcagaata aaaccgaaag tgaaaatacc
       61 agcgataaac cgaaacgtaa aaaaaaaggc ggtaaaaatg gtaaaaaccg ccgtaatcgt
      121 aaaaaaaaga atccgtgcaa tgcagaattt cagaattttt gtatccacgg cgaatgtaaa
      181 tatattgaac atctggaagc agtgacctgc aaatgtcagc aggaatattt tggcgaacgt
      241 tgcggcgaaa aaagcatgaa aacccatagt atgattgata gtagcctgag t
//
