LOCUS       sequence-386032-         258 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial production of soluble, active target proteins; Nterm 
            thrombin and enterokinase cleavage sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Ferreira AS, Lopacinski A, Batista M, Hiraiwa PM, Guimaraes BG, 
            Zanchin NIT
  TITLE     A toolkit for recombinant production of seven human EGF family 
            growth factors in active conformation.
  JOURNAL   Sci Rep. 2022 Mar 23;12(1):5034. doi: 10.1038/s41598-022-09060-9.
  PUBMED    35322149
REFERENCE   2  (bases 1 to 258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-03-23"; volume: "12(1):5034. doi"; pages: " 
            10.1038/s41598-022-09060-9"
COMMENT     Sequence Label: pET32a-hHBEGF
FEATURES             Location/Qualifiers
     source          1..258
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gatctgcagg aagccgatct ggatctgctg cgtgttaccc tgagtagcaa accgcaggcc
       61 ctggcaaccc cgaataaaga agaacatggc aaacgcaaaa aaaaaggtaa aggtctgggc
      121 aaaaaacgtg atccgtgcct gcgtaaatat aaagattttt gcattcacgg cgaatgcaaa
      181 tatgttaaag aactgcgtgc accgagttgc atttgtcatc cgggctatca tggcgaacgt
      241 tgtcatggcc tgagtctg
//
