LOCUS       sequence-386048-        9081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 NSP12_P314L in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 9081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 NSP12_P314L
FEATURES             Location/Qualifiers
     source          1..9081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(110..127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(281..869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(361..380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1040..1831,1832..1900))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1663..1682
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1901..2005)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2080..2099)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2271..2650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2651..2854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2805..2825
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2877..2895
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2905..2929
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3072..3096
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5854..5878)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5925..5948
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5985..6008
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6039..6065
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             6039..6065
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(6066..6413,6414..6416,6417..7025)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(7069..7086)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7075..7299
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7432..7451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7583..7630
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7638..8660
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7638..8660
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8790..8911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8827..8846)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8881..8900
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8960..8976)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8960..8976)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8973..8995)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8984..9000
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9008..9014,9015..9032,9033..9038))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9053..9074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
       61 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      721 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1021 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1081 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1141 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1201 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1261 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1321 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1381 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1441 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1501 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1561 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1621 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1681 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1741 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1801 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1861 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1921 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1981 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     2041 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     2101 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     2161 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     2221 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
     2281 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     2341 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2401 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2461 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2521 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2581 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2641 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2701 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2761 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2821 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
     2881 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
     2941 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     3001 agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc
     3061 tgcagatatc aacaagtttg tacaaaaaag ttggcatgtc cgccgctaga ctgactcctt
     3121 gtggtactgg cacctctact gacgtggtgt acagggcctt cgacatctac aacgacaagg
     3181 tggccggatt cgctaagttc ctgaagacca actgctgccg cttccaggag aaggacgaag
     3241 acgacaacct gatcgactct tacttcgtgg tgaagaggca caccttcagc aactaccagc
     3301 acgaggaaac catctacaac ctgctgaagg actgccccgc cgtggctaag cacgacttct
     3361 tcaagttccg catcgacggt gacatggtgc ctcacatcag ccgccagagg ctgaccaagt
     3421 acaccatggc cgacctggtg tacgctctga ggcacttcga cgagggcaac tgcgacaccc
     3481 tgaaggaaat cctggtgacc tacaactgct gcgacgacga ctacttcaac aagaaggact
     3541 ggtacgactt cgtggagaac cctgacatcc tgagagtgta cgccaacctg ggcgaaagag
     3601 tgcgtcaggc tctgctgaag accgtgcagt tctgcgacgc catgcgtaac gctggtatcg
     3661 tgggcgtgct gaccctggac aaccaggacc tgaacggcaa ctggtacgac ttcggagact
     3721 tcatccagac tactccagga tctggtgtgc ctgtggtgga ctcctactac tctctgctga
     3781 tgccaatcct gaccctgacc agggccctga ccgctgagtc tcacgtggac accgacctga
     3841 ccaagcccta catcaagtgg gacctgctga agtacgactt caccgaggaa cgcctgaagc
     3901 tgttcgacag gtacttcaag tactgggacc agacctacca cccaaactgc gtgaactgcc
     3961 tggacgacag atgcatcctg cactgcgcca acttcaacgt gctgttctct accgtgttcc
     4021 ctcccaccag cttcggccct ctggtgcgta agatcttcgt ggacggcgtg ccattcgtgg
     4081 tgtctaccgg ataccacttc agagagctgg gtgtggtgca caaccaggac gtgaacctgc
     4141 acagctcccg tctgagcttc aaggaactgc tggtgtacgc tgctgaccct gctatgcacg
     4201 ctgctagcgg caacctgctg ctggacaagc gcaccacctg cttctccgtg gccgctctga
     4261 ccaacaacgt ggccttccag accgtgaagc ctggaaactt caacaaggac ttctacgact
     4321 tcgctgtgag caagggattc ttcaaggagg gttctagcgt ggaactgaag cacttcttct
     4381 tcgcccagga cggcaacgcc gctatctccg actacgacta ctaccgctac aacctgccca
     4441 ccatgtgcga catcaggcag ctgctgttcg tggtggaggt ggtggacaag tacttcgact
     4501 gctacgacgg cggatgcatc aacgctaacc aggtcatcgt gaacaacctg gacaagagcg
     4561 ccggcttccc tttcaacaag tggggaaagg ctagactgta ctacgacagc atgtcctacg
     4621 aagaccagga cgccctgttc gcttacacca agcgtaacgt gatcccaacc atcacccaga
     4681 tgaacctgaa gtacgccatc tccgctaaga accgcgccag gaccgtggct ggtgtgagca
     4741 tctgctccac catgaccaac agacagttcc accagaagct gctgaagtct atcgccgcta
     4801 cccgtggtgc caccgtggtc atcggcacca gcaagttcta cggtggctgg cacaacatgc
     4861 tgaagaccgt gtactccgac gtggagaacc cacacctgat gggctgggac taccccaagt
     4921 gcgacagagc tatgcctaac atgctgcgta tcatggcctc tctggtgctg gctagaaagc
     4981 acaccacctg ctgctctctg agccaccgct tctacaggct ggccaacgag tgcgctcagg
     5041 tgctgtccga aatggtcatg tgcggaggtt ctctgtacgt gaagcccggc ggaacctcct
     5101 ctggagacgc taccaccgcc tacgctaact ccgtgttcaa catctgccag gccgtgaccg
     5161 ctaacgtgaa cgccctgctg tctaccgacg gtaacaagat cgctgacaag tacgtgagaa
     5221 acctgcagca ccgtctgtac gagtgcctgt acagaaaccg tgacgtggac accgacttcg
     5281 tgaacgaatt ctacgcctac ctgcgcaagc acttctccat gatgatcctg tctgacgacg
     5341 ctgtggtgtg cttcaactct acctacgcca gccagggcct ggtggctagc atcaagaact
     5401 tcaagtccgt gctgtactac cagaacaacg tgttcatgag cgaggccaag tgctggaccg
     5461 aaaccgacct gaccaaggga cctcacgagt tctgctccca gcacaccatg ctggtgaagc
     5521 agggtgacga ctacgtgtac ctgccatacc ctgacccttc tagaatcctg ggagctggat
     5581 gcttcgtgga cgacatcgtg aagaccgacg gcaccctgat gatcgaaagg ttcgtgagcc
     5641 tggccatcga cgcttaccct ctgaccaagc acccaaacca ggagtacgct gacgtgttcc
     5701 acctgtacct gcagtacatc cgcaagctgc acgacgaact gaccggacac atgctggaca
     5761 tgtacagcgt gatgctgacc aacgacaaca cctccaggta ctgggagcca gaattctacg
     5821 aggccatgta cacccctcac accgtgctgc agtacccaac tttcttgtac aaagtggttg
     5881 atatcaaggc cgccgacatc accagcctgt acaagaaagt ggggtggagc catccccagt
     5941 tcgagaaggg cggaggatct ggcggcggaa gcggcggagg aagctggtcc caccctcagt
     6001 ttgagaaagg caccgagctg ggcagcacca tggccagcta cccctacgac gtgcccgact
     6061 acgccaagga caacaccgtg cccctgaagc tgatcgccct gctggccaac ggcgagttcc
     6121 actctggcga gcagctgggc gagacactgg gcatgagcag agccgccatc aacaagcaca
     6181 tccagaccct gcgggactgg ggcgtggacg tgttcacagt gcccggcaag ggctacagcc
     6241 tgcccgagcc catccagctg ctgaacgcca agcagatcct gggccagctg gatggcggat
     6301 ctgtggccgt gctgcccgtg atcgacagca ccaaccagta tctgctcgac cggatcggcg
     6361 agctgaagtc cggcgacgcc tgtatcgccg agtaccagca ggccggcaga ggcggcaggg
     6421 gcagaaagtg gttcagcccc ttcggcgcca acctgtacct gagcatgttc tggagactgg
     6481 aacagggccc agccgccgct attggcctga gcctggtcat cggcatcgtg atggccgagg
     6541 tgctgcggaa gctgggcgcc gacaaagtgc gcgtgaagtg gcccaacgac ctgtatctgc
     6601 aggaccggaa gctggccggc atcctggtgg aactgaccgg caaaacaggc gacgccgccc
     6661 agatcgtgat cggcgctggc atcaacatgg ccatgcggcg ggtggaagag agcgtggtca
     6721 accagggctg gatcaccctg caggaagccg gcatcaacct ggaccggaac accctggccg
     6781 ccatgctgat cagagagctg agagccgccc tggaactgtt cgagcaggaa ggcctggccc
     6841 cctacctgag cagatgggag aagctggaca acttcatcaa ccggcccgtg aaactgatca
     6901 tcggcgacaa agagatcttc ggcatcagcc ggggcatcga caagcagggg gccctgctcc
     6961 tggaacagga cggcatcatc aagccctgga tgggcggcga gatcagcctg cggagcgccg
     7021 agaagtgatg actcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     7081 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     7141 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     7201 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     7261 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     7321 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     7381 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     7441 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7501 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7561 agggtgatgg ttcacgtacc tagaagttcc tattccgaag ttcctattct ctagaaagta
     7621 taggaacttc cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     7681 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     7741 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     7801 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     7861 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     7921 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     7981 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     8041 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     8101 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     8161 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     8221 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     8281 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     8341 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     8401 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     8461 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     8521 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     8581 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     8641 gtccgagggc aaaggaatag cacgtactac gagatttcga ttccaccgcc gccttctatg
     8701 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     8761 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     8821 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8881 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     8941 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     9001 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     9061 gctaactcac attaattgcg t
//
