LOCUS       sequence-38605-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Vick JE, Johnson ET, Choudhary S, Bloch SE, Lopez-Gallego F, 
            Srivastava P, Tikh IB, Wawrzyn GT, Schmidt-Dannert C
  TITLE     Optimized compatible set of BioBrick vectors for metabolic pathway 
            engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
  PUBMED    22033566
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2011-12"; volume: "92(6)"; pages: 
            "1275-86. Epub 2011 Oct 28"
COMMENT     Sequence Label: pUCBB-pBAD-eGFP
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(70..354)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(128..147)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 cctcgccctt gctcaccata tggatccaga tctcttaaac aaaattattt ctagcccaaa
       61 aaaacgggta tggagaaaca gtagagagtt gcgataaaaa gcgtcaggta ggatccgcta
      121 atcttatgga taaaaatgct atggcatagc aaagtgtgac gccgtgcaaa taatcaatgt
      181 ggacttttct gccgtgatta tagacacttt tgttacgcgt ttttgtcatg gctttggtcc
      241 cgctttgtta cagaatgctt ttaataagcg gggttaccgg tttggttagc gagaagagcc
      301 agtaaaagac gcagtgacgg caatgtctga tgcaatatgg acaattggtt tcttctctga
      361 atggcgggag tatgaaaagt atggctgaag cgcaaaatga tcccctgctg ccgggatact
      421 cgtttaatgc ccatctggtg gcgggtttaa cgccgattga ggccaacggt tatctcgatt
      481 tttttatcga ccgaccgctg ggaatgaaag gttatattct caatctcacc attcgcggtc
      541 agggggtggt gaaaaatcag ggacgagaat ttgtttgccg accgggtgat attttgctgt
      601 tcccgccagg agagattcat cactacggtc gtcatccgga ggctcgcgaa tggtatcacc
      661 agtgggttta ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg ccgtcaatat
      721 ttgccaatac ggggttcttt cgcccggatg aagcgcacca gccgcatttc agcgacctgt
      781 ttgggcaaat cattaacgcc gggcaagggg aagggcgcta ttcggagctg ctggcgataa
      841 atctgcttga gcaattgtta ctgcggcgca tggaagcgat taacgagtcg ctccatccac
      901 cg
//
