LOCUS       sequence-386057-        8130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 NSP13_P77L in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 8130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 NSP13_P77L
FEATURES             Location/Qualifiers
     source          1..8130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(110..127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(281..869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(361..380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1040..1831,1832..1900))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1663..1682
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1901..2005)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2080..2099)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2271..2650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2651..2854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2805..2825
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2877..2895
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2905..2929
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3072..3096
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4903..4927)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4974..4997
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5034..5057
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5088..5114
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5088..5114
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(5115..5462,5463..5465,5466..6074)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(6118..6135)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6124..6348
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6481..6500)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6632..6679
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6687..7709
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6687..7709
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7839..7960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7876..7895)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7930..7949
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8009..8025)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8009..8025)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8022..8044)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8033..8049
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8057..8063,8064..8081,8082..8087))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8102..8123
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
       61 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      721 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1021 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1081 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1141 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1201 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1261 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1321 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1381 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1441 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1501 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1561 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1621 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1681 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1741 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1801 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1861 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1921 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1981 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     2041 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     2101 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     2161 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     2221 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
     2281 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     2341 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2401 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2461 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2521 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2581 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2641 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2701 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2761 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2821 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
     2881 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
     2941 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     3001 agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc
     3061 tgcagatatc aacaagtttg tacaaaaaag ttggcatggc cgtgggcgct tgcgtgctgt
     3121 gcaactctca gaccagcctg agatgtggag cttgcatccg caggcctttc ctgtgctgca
     3181 agtgctgcta cgaccacgtg atcagcacct cccacaagct ggtgctgtcc gtgaacccat
     3241 acgtgtgcaa cgctcctggt tgtgacgtga ctgacgtgac ccagctgtac ctgggcggaa
     3301 tgagctacta ctgcaagtcc cacaagctcc ctatcagctt cccactgtgc gctaacggcc
     3361 aggtgttcgg actgtacaag aacacctgcg tgggctccga caacgtgacc gacttcaacg
     3421 ccatcgctac ctgcgactgg accaacgccg gagactacat cctggctaac acctgcaccg
     3481 aaaggctgaa gctgttcgcc gctgagaccc tgaaggccac cgaggaaacc ttcaagctgt
     3541 cttacggtat cgctaccgtg cgtgaggtgc tgtctgaccg cgaactgcac ctgtcttggg
     3601 aagtgggcaa gccaagacca cccctgaacc gtaactacgt gttcaccgga tacagggtga
     3661 ccaagaacag caaggtgcag atcggagagt acaccttcga aaagggagac tacggtgacg
     3721 ccgtggtgta cagaggaacc accacctaca agctgaacgt gggtgactac ttcgtgctga
     3781 cctcccacac cgtgatgcca ctgtctgctc ccaccctggt gcctcaggaa cactacgtgc
     3841 gcatcaccgg actgtaccca accctgaaca tctccgacga gttcagctcc aacgtggcca
     3901 actaccagaa agtgggcatg cagaagtact ccaccctgca gggacctcca ggtaccggca
     3961 agtctcactt cgccatcggt ctggctctgt actacccctc tgcccgtatc gtgtacactg
     4021 cttgttctca cgctgctgtg gatgctctgt gcgagaaggc tctgaagtac ctgcctatcg
     4081 acaagtgctc ccgcatcatc ccagccagag ctcgtgtgga atgcttcgac aagttcaagg
     4141 tgaactctac cctggagcag tacgtgttct gcaccgtgaa cgccctgcct gaaaccaccg
     4201 ctgacatcgt ggtgttcgac gagatcagca tggccaccaa ctacgacctg tctgtggtga
     4261 acgctagact gagggctaag cactacgtgt acatcggtga cccagctcag ctgcctgctc
     4321 ctcgtaccct gctgaccaag ggcaccctgg agccagaata cttcaacagc gtgtgccgcc
     4381 tgatgaagac catcggtcca gacatgttcc tgggtacctg ccgccgctgc cctgctgaaa
     4441 tcgtggacac cgtgtctgct ctggtgtacg acaacaagct gaaggcccac aaggacaaga
     4501 gcgcccagtg cttcaagatg ttctacaagg gagtgatcac ccacgacgtg tctagcgcca
     4561 tcaacaggcc ccagatcggt gtggtgcgcg agttcctgac caggaacccc gcctggagaa
     4621 aggccgtgtt catctcccct tacaactctc agaacgccgt ggcttccaag atcctgggtc
     4681 tgcctaccca gaccgtggac tcctctcagg gctctgaata cgactacgtg atcttcaccc
     4741 agaccaccga gaccgcccac agctgcaacg tgaacaggtt caacgtggcc atcaccagag
     4801 ctaaagtggg aatcctgtgc atcatgagcg acagagacct gtacgacaag ctgcagttca
     4861 cctccctgga gatcccccgc aggaacgtgg ctaccctgca gtacccaact ttcttgtaca
     4921 aagtggttga tatcaaggcc gccgacatca ccagcctgta caagaaagtg gggtggagcc
     4981 atccccagtt cgagaagggc ggaggatctg gcggcggaag cggcggagga agctggtccc
     5041 accctcagtt tgagaaaggc accgagctgg gcagcaccat ggccagctac ccctacgacg
     5101 tgcccgacta cgccaaggac aacaccgtgc ccctgaagct gatcgccctg ctggccaacg
     5161 gcgagttcca ctctggcgag cagctgggcg agacactggg catgagcaga gccgccatca
     5221 acaagcacat ccagaccctg cgggactggg gcgtggacgt gttcacagtg cccggcaagg
     5281 gctacagcct gcccgagccc atccagctgc tgaacgccaa gcagatcctg ggccagctgg
     5341 atggcggatc tgtggccgtg ctgcccgtga tcgacagcac caaccagtat ctgctcgacc
     5401 ggatcggcga gctgaagtcc ggcgacgcct gtatcgccga gtaccagcag gccggcagag
     5461 gcggcagggg cagaaagtgg ttcagcccct tcggcgccaa cctgtacctg agcatgttct
     5521 ggagactgga acagggccca gccgccgcta ttggcctgag cctggtcatc ggcatcgtga
     5581 tggccgaggt gctgcggaag ctgggcgccg acaaagtgcg cgtgaagtgg cccaacgacc
     5641 tgtatctgca ggaccggaag ctggccggca tcctggtgga actgaccggc aaaacaggcg
     5701 acgccgccca gatcgtgatc ggcgctggca tcaacatggc catgcggcgg gtggaagaga
     5761 gcgtggtcaa ccagggctgg atcaccctgc aggaagccgg catcaacctg gaccggaaca
     5821 ccctggccgc catgctgatc agagagctga gagccgccct ggaactgttc gagcaggaag
     5881 gcctggcccc ctacctgagc agatgggaga agctggacaa cttcatcaac cggcccgtga
     5941 aactgatcat cggcgacaaa gagatcttcg gcatcagccg gggcatcgac aagcaggggg
     6001 ccctgctcct ggaacaggac ggcatcatca agccctggat gggcggcgag atcagcctgc
     6061 ggagcgccga gaagtgatga ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct
     6121 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     6181 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     6241 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     6301 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     6361 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     6421 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6481 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6541 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6601 aacttgatta gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc
     6661 tagaaagtat aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga
     6721 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     6781 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     6841 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     6901 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     6961 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     7021 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     7081 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     7141 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     7201 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     7261 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     7321 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     7381 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     7441 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     7501 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     7561 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     7621 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     7681 ccagcactcg tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg
     7741 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     7801 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     7861 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7921 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     7981 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     8041 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8101 aatgagtgag ctaactcaca ttaattgcgt
//
