LOCUS       sequence-386069-       12162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 NSP3_K977Q in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12162)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 12162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 NSP3_K977Q
FEATURES             Location/Qualifiers
     source          1..12162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(110..127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(281..869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(361..380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1040..1831,1832..1900))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1663..1682
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1901..2005)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2080..2099)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2271..2650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2651..2854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2805..2825
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2877..2895
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2905..2929
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3072..3096
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8935..8959)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9006..9029
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             9066..9089
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             9120..9146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             9120..9146
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(9147..9494,9495..9497,9498..10106)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(10150..10167)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10156..10380
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10513..10532)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    10664..10711
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             10719..11741
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             10719..11741
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    11871..11992
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11908..11927)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11962..11981
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12041..12057)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12041..12057)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12054..12076)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12065..12081
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12089..12095,12096..12113,12114..12119))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12134..12155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
       61 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      721 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1021 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1081 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1141 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1201 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1261 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1321 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1381 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1441 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1501 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1561 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1621 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1681 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1741 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1801 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1861 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1921 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1981 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     2041 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     2101 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     2161 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     2221 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
     2281 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     2341 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2401 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2461 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2521 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2581 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2641 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2701 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2761 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2821 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
     2881 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
     2941 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     3001 agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc
     3061 tgcagatatc aacaagtttg tacaaaaaag ttggcatggc ccctaccaag gtgaccttcg
     3121 gagacgacac cgtgatcgag gtgcagggtt acaagagcgt gaacatcacc ttcgagctgg
     3181 acgaacgcat cgacaaggtg ctgaacgaaa agtgctccgc ttacaccgtg gagctgggca
     3241 ccgaggtgaa cgaattcgcc tgcgtggtgg ccgacgctgt gatcaagacc ctgcagccag
     3301 tgtccgaact gctgacccct ctgggtatcg acctggacga gtggtctatg gctacctact
     3361 acctgttcga cgagagcggc gaattcaagc tggcctctca catgtactgc agcttctacc
     3421 ctcccgacga agacgaggaa gagggtgact gcgaagagga agagttcgag ccttccactc
     3481 agtacgagta cggcaccgaa gacgactacc agggcaagcc tctggaattc ggagccacct
     3541 ctgccgctct gcagcctgaa gaggaacagg aggaagactg gctggacgac gactcccagc
     3601 agaccgtggg tcagcaggac ggctctgaag acaaccagac caccaccatc cagaccatcg
     3661 tggaggtgca gcctcagctg gagatggaac tgaccccagt ggtgcagacc atcgaggtga
     3721 acagcttctc cggctacctg aagctgaccg acaacgtgta catcaagaac gctgacatcg
     3781 tggaggaagc caagaaggtg aaaccaaccg tggtggtgaa cgccgctaac gtgtacctga
     3841 agcacggcgg aggtgtggct ggagctctga acaaggctac caacaacgcc atgcaggtgg
     3901 aatccgacga ctacatcgct accaacggtc ctctgaaggt gggcggatcc tgcgtgctgt
     3961 ctggccacaa cctggccaag cactgcctgc acgtggtggg accaaacgtg aacaagggag
     4021 aggacatcca gctgctgaag agcgcctacg aaaacttcaa ccagcacgag gtgctgctgg
     4081 ctccactgct gtctgccgga atcttcggtg ctgatcccat ccacagcctg cgcgtgtgcg
     4141 tggacaccgt gaggaccaac gtgtacctgg ccgtgttcga caagaacctg tacgacaagc
     4201 tggtgagctc cttcctggag atgaagtccg aaaagcaggt ggagcagaag atcgccgaga
     4261 tccccaagga ggaagtgaag cctttcatca ccgaatctaa gcccagcgtg gagcagagaa
     4321 agcaggacga caagaagatc aaggcctgcg tggaggaagt gaccaccacc ctggaggaaa
     4381 ccaagttcct gaccgagaac ctgctgctgt acatcgacat caacggcaac ctgcaccctg
     4441 actccgctac cctggtgtct gacatcgaca tcaccttcct gaagaaggac gccccctaca
     4501 tcgtgggcga cgtggtgcag gaaggagtgc tgaccgctgt ggtcatccct accaagaagg
     4561 ccggtggcac caccgagatg ctggccaagg ctctgcgtaa ggtgccaacc gacaactaca
     4621 tcaccaccta ccccggtcag ggcctgaacg gttacaccgt ggaggaagct aagaccgtgc
     4681 tgaagaagtg caagtctgcc ttctacatcc tgccctccat catctctaac gagaagcagg
     4741 aaatcctggg caccgtgagc tggaacctgc gcgaaatgct ggcccacgct gaggaaacca
     4801 ggaagctgat gcccgtgtgc gtggagacca aggccatcgt gagcaccatc cagagaaagt
     4861 acaagggcat caagatccag gagggtgtgg tggactacgg cgctcgtttc tacttctaca
     4921 cctccaagac caccgtggcc tctctgatca acaccctgaa cgacctgaac gagaccctgg
     4981 tgaccatgcc actgggatac gtgacccacg gtctgaacct ggaggaagcc gctagataca
     5041 tgcgttccct gaaggtgcct gctaccgtga gcgtgagcag ccctgacgct gtgaccgcct
     5101 acaacggata cctgacctcc tctagcaaga cccctgagga acacttcatc gaaaccatca
     5161 gcctggccgg ttcctacaag gactggtctt acagcggaca gtccacccag ctgggcatcg
     5221 agttcctgaa gcgcggagac aagagcgtgt actacacctc caacccaacc accttccacc
     5281 tggacggtga agtgatcacc ttcgacaacc tgaagaccct gctgtctctg cgcgaggtga
     5341 ggaccatcaa ggtgttcacc accgtggaca acatcaacct gcacacccag gtggtggaca
     5401 tgagcatgac ctacggacag cagttcggtc ctacctacct ggacggagct gacgtgacca
     5461 agatcaagcc acacaactcc cacgagggca agaccttcta cgtgctgcct aacgatgaca
     5521 ccctgagagt ggaggctttc gaatactacc acaccaccga cccatccttc ctgggcaggt
     5581 acatgtctgc cctgaaccac accaagaagt ggaagtaccc ccaggtgaac ggactgacca
     5641 gcatcaagtg ggccgacaac aactgctacc tggccaccgc tctgctgacc ctgcagcaga
     5701 tcgaactgaa gttcaaccca cccgctctgc aggacgctta ctacagagcc cgtgctggcg
     5761 aggccgctaa cttctgcgct ctgatcctgg cctactgcaa caagaccgtg ggcgaactgg
     5821 gagacgtgag agagaccatg agctacctgt tccagcacgc caacctggac tcctgcaagc
     5881 gtgtgctgaa cgtggtgtgc aagacctgcg gccagcagca gaccaccctg aagggagtgg
     5941 aagctgtgat gtacatgggt accctgtcct acgagcagtt caagaagggc gtgcagatcc
     6001 cttgcacctg cggaaagcag gccacccagt acctggtgca gcaggaaagc ccattcgtga
     6061 tgatgtccgc tcctccagcc cagtacgaac tgaagcacgg caccttcacc tgcgcttccg
     6121 agtacaccgg caactaccag tgcggacact acaagcacat cacctctaag gagaccctgt
     6181 actgcatcga cggagccctg ctgaccaagt cctctgaata caagggtccc atcaccgacg
     6241 tgttctacaa ggagaacagc tacaccacca ccatcaagcc cgtgacctac aagctggacg
     6301 gcgtggtgtg caccgaaatc gaccctaagc tggacaacta ctacaagaag gacaactctt
     6361 acttcaccga gcagcctatc gacctggtgc caaaccagcc ctaccctaac gccagcttcg
     6421 acaacttcaa gttcgtgtgc gacaacatca agttcgctga cgacctgaac cagctgaccg
     6481 gatacaagaa gcctgcctct agagagctga aggtgacctt cttcccagac ctgaacggcg
     6541 acgtggtggc tatcgactac aagcactaca ccccaagctt caagaaggga gccaagctgc
     6601 tgcacaagcc catcgtgtgg cacgtgaaca acgctaccaa caaggccacc tacaagccta
     6661 acacctggtg catccgttgc ctgtggtcta ccaagccagt ggaaacctcc aactctttcg
     6721 acgtgctgaa gagcgaggac gctcagggca tggacaacct ggcctgcgaa gacctgaagc
     6781 ccgtgagcga ggaagtggtg gagaacccaa ccatccagaa ggacgtgctg gaatgcaacg
     6841 tgaagaccac cgaggtggtg ggagacatca tcctgaagcc agctaacaac agcctgaaga
     6901 tcaccgagga agtgggtcac accgacctga tggccgctta cgtggacaac agctccctga
     6961 ccatcaagaa gcccaacgag ctgagcaggg tgctgggtct gaagaccctg gctactcacg
     7021 gtctggctgc tgtgaactct gtgccatggg acaccatcgc taactacgcc aagcctttcc
     7081 tgaacaaggt ggtgtccacc accaccaaca tcgtgaccag atgtctgaac cgcgtgtgca
     7141 ccaactacat gccctacttc ttcaccctgc tgctgcagct gtgcaccttc accagaagca
     7201 ccaactcccg tatcaaggct agcatgccta ccaccatcgc caagaacacc gtgaagagcg
     7261 tgggaaagtt ctgcctggag gcttccttca actacctgaa gtctccaaac ttcagcaagc
     7321 tgatcaacat catcatctgg ttcctgctgc tctccgtgtg cctgggttcc ctgatctact
     7381 ctaccgccgc tctgggagtg ctgatgtcta acctgggtat gcctagctac tgcactggtt
     7441 acagagaggg atacctgaac agcaccaacg tgaccatcgc cacctactgc accggcagca
     7501 tcccatgctc cgtgtgcctg tctggactgg acagcctgga cacctacccc tccctggaaa
     7561 ccatccagat caccatctct agcttcaagt gggacctgac cgccttcggt ctggtggctg
     7621 agtggttcct ggcctacatc ctgttcacca ggttcttcta cgtgctgggc ctggccgcta
     7681 tcatgcagct gttcttctcc tacttcgctg tgcacttcat cagcaactcc tggctgatgt
     7741 ggctgatcat caacctggtg cagatggctc ccatctctgc catggtgcgc atgtacatct
     7801 tcttcgccag cttctactac gtgtggaagt cctacgtgca cgtggtggac ggttgcaact
     7861 cctctacctg catgatgtgc tacaagcgca acagggctac cagggtggag tgcaccacca
     7921 tcgtgaacgg cgtgcgcagg tccttctacg tgtacgccaa cggcggcaag ggattctgca
     7981 agctgcacaa ctggaactgc gtgaactgcg acaccttctg cgctggctcc accttcatct
     8041 ctgacgaggt ggccagagac ctgagcctgc agttcaagcg tccaatcaac cccaccgacc
     8101 agagctccta catcgtggac tctgtgaccg tgaagaacgg tagcatccac ctgtacttcg
     8161 acaaggctgg ccagaagacc tacgagcgcc actctctgag ccacttcgtg aacctggaca
     8221 acctgagggc caacaacacc aagggcagcc tgcctatcaa cgtgatcgtg ttcgacggaa
     8281 agtccaagtg cgaggaatct agcgccaagt ccgccagcgt gtactacagc cagctgatgt
     8341 gccagccaat cctgctgctg gaccaggctc tggtgtctga cgtgggagac agcgccgaag
     8401 tggccgtgaa gatgttcgac gcctacgtga acaccttctc ctctaccttc aacgtgccta
     8461 tggagaagct gaagaccctg gtggctactg ctgaggctga actggctaag aacgtgtccc
     8521 tggacaacgt gctgagcacc ttcatctccg ccgctaggca gggtttcgtg gactctgacg
     8581 tggaaaccaa agacgtggtg gagtgcctga agctgtctca ccagagcgac atcgaagtga
     8641 ccggcgactc ctgcaacaac tacatgctga cctacaacaa ggtggagaac atgaccccaa
     8701 gagacctggg agcttgcatc gactgctccg cccgtcacat caacgctcag gtggccaagt
     8761 ctcacaacat cgccctgatc tggaacgtga aggacttcat gagcctgtcc gagcagctgc
     8821 gcaagcagat caggagcgcc gctaagaaga acaacctgcc cttcaagctg acctgcgcta
     8881 ccaccagaca ggtggtgaac gtggtgacca ccaagatcgc cctgaagggc ggatacccaa
     8941 ctttcttgta caaagtggtt gatatcaagg ccgccgacat caccagcctg tacaagaaag
     9001 tggggtggag ccatccccag ttcgagaagg gcggaggatc tggcggcgga agcggcggag
     9061 gaagctggtc ccaccctcag tttgagaaag gcaccgagct gggcagcacc atggccagct
     9121 acccctacga cgtgcccgac tacgccaagg acaacaccgt gcccctgaag ctgatcgccc
     9181 tgctggccaa cggcgagttc cactctggcg agcagctggg cgagacactg ggcatgagca
     9241 gagccgccat caacaagcac atccagaccc tgcgggactg gggcgtggac gtgttcacag
     9301 tgcccggcaa gggctacagc ctgcccgagc ccatccagct gctgaacgcc aagcagatcc
     9361 tgggccagct ggatggcgga tctgtggccg tgctgcccgt gatcgacagc accaaccagt
     9421 atctgctcga ccggatcggc gagctgaagt ccggcgacgc ctgtatcgcc gagtaccagc
     9481 aggccggcag aggcggcagg ggcagaaagt ggttcagccc cttcggcgcc aacctgtacc
     9541 tgagcatgtt ctggagactg gaacagggcc cagccgccgc tattggcctg agcctggtca
     9601 tcggcatcgt gatggccgag gtgctgcgga agctgggcgc cgacaaagtg cgcgtgaagt
     9661 ggcccaacga cctgtatctg caggaccgga agctggccgg catcctggtg gaactgaccg
     9721 gcaaaacagg cgacgccgcc cagatcgtga tcggcgctgg catcaacatg gccatgcggc
     9781 gggtggaaga gagcgtggtc aaccagggct ggatcaccct gcaggaagcc ggcatcaacc
     9841 tggaccggaa caccctggcc gccatgctga tcagagagct gagagccgcc ctggaactgt
     9901 tcgagcagga aggcctggcc ccctacctga gcagatggga gaagctggac aacttcatca
     9961 accggcccgt gaaactgatc atcggcgaca aagagatctt cggcatcagc cggggcatcg
    10021 acaagcaggg ggccctgctc ctggaacagg acggcatcat caagccctgg atgggcggcg
    10081 agatcagcct gcggagcgcc gagaagtgat gactcgagtc tagagggccc gtttaaaccc
    10141 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
    10201 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
    10261 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
    10321 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
    10381 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
    10441 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
    10501 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
    10561 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
    10621 tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa
    10681 gttcctattc tctagaaagt ataggaactt ccttggccaa aaagcctgaa ctcaccgcga
    10741 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
    10801 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
    10861 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
    10921 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
    10981 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
    11041 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
    11101 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
    11161 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
    11221 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
    11281 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
    11341 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
    11401 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
    11461 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
    11521 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
    11581 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
    11641 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
    11701 gaaaccgacg ccccagcact cgtccgaggg caaaggaata gcacgtacta cgagatttcg
    11761 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
    11821 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
    11881 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    11941 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    12001 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
    12061 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    12121 cctggggtgc ctaatgagtg agctaactca cattaattgc gt
//
