LOCUS       sequence-386077-       10933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10933)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PAPLN(Trunc1)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             4772..4807
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             4817..4840
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6500..6544
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6545..6571
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(6711..6728)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6717..6828
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6946..6966)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6951..7280
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7131..7266
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7193..7212
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7328..7375
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7394..7993
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7394..7413)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7890..7910
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8123..8244
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8160..8179)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8214..8233
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8297..9088,9089..9157))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8920..8939
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9158..9249)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9291..9310
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9316..9335
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9421..10009
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9910..9929
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10163..10180
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     10278..10295
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10279..10295
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10751..10767
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg ccgctcctaa agttcggaga cagtctgata cctggggacc
     1021 ttggagtcag tggtcccctt gcagcagaac atgtggcggc ggagtgtcct tcagagagag
     1081 gccttgttac agccagcgga gagatggcgg cagctcttgt gttggacctg ccagaagcca
     1141 cagaagctgc agaaccgaga gctgccctga tggcgccaga gatttcagag ccgagcagtg
     1201 cgccgagttc gatggcgctg aattccaagg cagacggtac agatggctgc cctactacag
     1261 cgcccctaac aagtgcgagc tgaactgcat ccccaagggc gagaacttct actacaagca
     1321 ccgggaagcc gtggtggatg gcaccccttg tgaacctggc aagagggatg tgtgtgtgga
     1381 cggcagctgt agagtcgtgg gctgtgatca cgagctggac agcagcaagc aagaggacaa
     1441 gtgcctgaga tgcggaggcg acggcaccac atgttatcct gtggccggca ccttcgacgc
     1501 caacgatctt agcagaggct acaaccagat cctgatcgtg cctatgggcg ccaccagcat
     1561 tctgatcgat gaagccgctg ccagccggaa ctttctggcc gtgaaaaatg tgcggggcga
     1621 gtactacctg aacggccact ggacaatcga ggccgctaga gcacttcctg ccgcctctac
     1681 aatcctgcac tatgagagag gcgccgaggg cgatctggct cctgaaagac ttcatgccag
     1741 aggacccacc agcgagcctc tggttatcga gctgatctcc caagagccta atcctggcgt
     1801 gcactacgag taccatctgc ctctgagaag gccctctcct ggcttctctt ggagccacgg
     1861 atcttggagc gattgctctg ccgaatgtgg cggaggacac cagagcagac tggtgttctg
     1921 caccatcgac cacgaggctt accccgacca catgtgccag agacagccta gacctgccga
     1981 caggcggagc tgtaatctgc acccttgtcc tgagacaaag cggtggaaag ccggaccttg
     2041 ggctccttgt tctgcctctt gcggcggagg atctcagtcc agaagcgtgt actgcatcag
     2101 ctccgatggc gccggaatcc aagaggctgt ggaagaggct gaatgtgccg gcctgcctgg
     2161 aaagcctcct gccattcagg cttgcaacct gcagagatgt gccgcttgga gccctgagcc
     2221 ttggggcgaa tgttctgtgt cttgtggcgt gggcgtcaga aagcggagcg tgacatgcag
     2281 aggcgagaga ggatctctgc tgcacacagc cgcctgcagc ctggaagata gacctcctct
     2341 gaccgagcct tgcgtgcacg aggattgtcc tctgctgtct gatcaggcct ggcacgttgg
     2401 aacatggggc ctgtgtagca agagctgtag cagcggaacc cggcggagac aagtgatctg
     2461 tgccatcgga cctccaagcc actgtggaag cctgcagcac agcaagcccg tggatgtgga
     2521 accttgcaac acccagcctt gccatctgcc acaagaggtg cccagcatgc aggatgtgca
     2581 cacccctgcc agcaatccct ggatgccact gggacctcaa gagagcccag ccagcgatag
     2641 tagaggacag tggtgggccg ctcaagagca cccttctgct agaggcgatc acagaggcga
     2701 aaggggcgat cccaggggag atcaaggcac acatctgtct gctctcggcc ctgcaccttc
     2761 tctgcagcag cctccttatc agcagccact gagaagcggc tctggccctc acgattgcag
     2821 acactctcca cacggctgtt gcccagatgg ccacacagca tctctgggcc ctcaatggca
     2881 aggatgtcct ggcgctcctt gccagcagag cagatacgga tgctgccccg atagagtgtc
     2941 cgtggccgaa ggacctcatc acgccggctg tacaaagagc tacggcggcg attctacagg
     3001 cggcatgcct agaagtagag ccgtggccag caccgtgcac aacactcatc agcctcaggc
     3061 tcagcagaac gagccctctg agtgtagagg cagccagttc ggctgctgct acgacaatgt
     3121 ggctacagcc gctggacctc tcggcgaagg atgtgtggga cagccttctc acgcttaccc
     3181 tgtgcgttgt ctgctgcctt ctgctcacgg ctcttgtgcc gattgggccg ccagatggta
     3241 tttcgtggcc tctgtgggcc agtgcaaccg gttttggtac ggcggctgtc acggcaacgc
     3301 caacaatttc gccagcgagc aagagtgcat gagcagctgt cagggctctc tgcacggacc
     3361 tagaaggcct caacctggcg cctctggcag atctacacac acagatggtg gcggatctag
     3421 ccctgccggc gaacaagaac ctagccagca tagaacaggc gccgctgtgc agaggaagcc
     3481 ttggccttct ggcggacttt ggagacagga ccagcaacct ggacctggcg aagcccctca
     3541 cacacaggct tttggagaat ggccatgggg ccaagagctg ggatcaagag cccctggact
     3601 tggcggagat gctggatctc ctgctcctcc atttcacagc agcagctaca gaatcagcct
     3661 ggccggcgtg gaaccatctc tggttcaagc tgctctggga cagctcgtgc ggctgagctg
     3721 ttccgatgat acagcccctg aatctcaggc cgcctggcag aaagatggcc agccaatcag
     3781 cagcgaccgg cacagactgc agtttgacgg cagcctgatc attcacccac tgcaggctga
     3841 ggacgccgga acatatagct gcggctctac cagacctggc cgggactctc agaagatcca
     3901 gctgagaatc atcggcggcg acatggccgt tctgtctgag gccgaactga gcagattccc
     3961 tcagcctaga gatcccgctc aggattttgg acaggctggc gctgctggac cactgggagc
     4021 tattccatct tctcaccctc agccagccaa ccggctgaga ctggaccaga atcaacccag
     4081 agtggtggac gcctctccag gccagagaat cagaatgacc tgtagagccg agggcttccc
     4141 accaccagct atcgagtggc aacgagatgg gcagcctgtg tctagcccaa gacatcagct
     4201 gcagccagat ggcagcctcg tgattagcag agtggccgtg gaagatggcg gattctacac
     4261 atgcgtggcc ttcaacggcc aggaccggga tcagagatgg gtgcagctga gagtgctggg
     4321 cgagctgaca atttctggcc tgcctcctac cgtgacagtg ccagagggcg atacagccag
     4381 actgctgtgt gtggtggctg gcgagtccgt gaacatccgg tggtccagaa atggcctgcc
     4441 agtgcaggcc gatggccata gagttcacca gtctccagac ggcaccctgc tgatctacaa
     4501 cctgagagcc agagatgagg gctcctacac ctgtagcgcc taccagggtt ctcaggctgt
     4561 gtccagatcc accgaagtga aggtggtgtc ccctgctcct acagcacagc cacgagatcc
     4621 cggcagagac tgtgtggatc aacccgagct ggccaactgc gacctgattc tgcaggccca
     4681 gctgtgcggc aatgagtact acagcagctt ttgctgcgcc agctgctcca gattccagcc
     4741 acacgctcag cccatctggc aaggcgcgcc ggaagatcaa gttgatccaa gattgattga
     4801 tggcaagggc ggatctctgg aggtgctgtt ccagggacca ggcggaagcg gcaatcccgg
     4861 cggcgatctg gccccccaga tgctgcgcga gctgcaggag accaatgccg ccctgcagga
     4921 cgtgcgcgag ctgctgcgcc accgcgtgaa ggagatcacc ttcctgaaga acaccgtgat
     4981 ggagtgcgac gcctgcggta tgcagccctc tagaggctcc ggctccatca tcccagttga
     5041 ggaggagaac ccggacttct ggaaccgcga ggcagccgag gccctgggtg ccgccaagaa
     5101 gctgcagcct gcacagacag ccgccaagaa cctcatcatc ttcctgggcg atgggatggg
     5161 ggtgtctacg gtgacagctg ccaggatcct aaaagggcag aagaaggaca aactggggcc
     5221 tgagataccc ctggccatgg accgcttccc atatgtggct ctgtccaaga catacaatgt
     5281 agacaaacat gtgccagaca gtggagccac agccacggcc tacctgtgcg gggtcaaggg
     5341 caacttccag accattggct tgagtgcagc cgcccgcttt aaccagtgca acacgacacg
     5401 cggcaacgag gtcatctccg tgatgaatcg ggccaagaaa gcagggaagt cagtgggagt
     5461 ggtaaccacc acacgagtgc agcacgcctc gccagccggc acctacgccc acacggtgaa
     5521 ccgcaactgg tactcggacg ccgacgtgcc tgcctcggcc cgccaggagg ggtgccagga
     5581 catcgctacg cagctcatct ccaacatgga cattgacgtg atcctaggtg gaggccgaaa
     5641 gtacatgttt cgcatgggaa ccccagaccc tgagtaccca gatgactaca gccaaggtgg
     5701 gaccaggctg gacgggaaga atctggtgca ggaatggctg gcgaagcgcc agggtgcccg
     5761 gtatgtgtgg aaccgcactg agctcatgca ggcttccctg gacccgtctg tgacccatct
     5821 catgggtctc tttgagcctg gagacatgaa atacgagatc caccgagact ccacactgga
     5881 cccctccctg atggagatga cagaggctgc cctgcgcctg ctgagcagga acccccgcgg
     5941 cttcttcctc ttcgtggagg gtggtcgcat cgaccatggt catcatgaaa gcagggctta
     6001 ccgggcactg actgagacga tcatgttcga cgacgccatt gagagggcgg gccagctcac
     6061 cagcgaggag gacacgctga gcctcgtcac tgccgaccac tcccacgtct tctccttcgg
     6121 aggctacccc ctgcgaggga gctccatctt cgggctggcc cctggcaagg cccgggacag
     6181 gaaggcctac acggtcctcc tatacggaaa cggtccaggc tatgtgctca aggacggcgc
     6241 ccggccggat gttaccgaga gcgagagcgg gagccccgag tatcggcagc agtcagcagt
     6301 gcccctggac gaagagaccc acgcaggcga ggacgtggcg gtgttcgcgc gcggcccgca
     6361 ggcgcacctg gttcacggcg tgcaggagca gaccttcata gcgcacgtca tggccttcgc
     6421 cgcctgcctg gagccctaca ccgcctgcga cctggcgccc cccgccggca ccaccgacgc
     6481 cgcgcacccg ggtggcggag gactgaacga catcttcgag gctcagaaaa tcgaatggca
     6541 cgaacatcac catcaccatc accatcacca ttaaggccgg cctgtagtca ctggcacaac
     6601 gcgttacccg gtaagccaat cgggtataca cggtcgtcat actgcagaca gggttcttct
     6661 actttgcaag atagtcttga gtagtaaaat aaatagatag agaaaatcag cctcgactgt
     6721 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     6781 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcac aacactcaac
     6841 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6901 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     6961 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7021 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7081 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     7141 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7201 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7261 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     7321 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     7381 gaggaactaa accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     7441 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     7501 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     7561 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     7621 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     7681 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     7741 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     7801 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     7861 gcccgccttc ctggagacat ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     7921 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     7981 gcccggtgcc tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     8041 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     8101 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     8161 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8221 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     8281 ggcgtaatca tggtcattac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8341 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8401 gcttaccatc tggccccagc gctgcgatga taccgcgaga accacgctca ccggctccgg
     8461 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8521 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8581 ttaatagttt gcgcaacgtt gttgccatcg ctacaggcat cgtggtgtca cgctcgtcgt
     8641 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8701 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8761 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8821 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8881 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8941 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9001 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9061 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9121 agggaataag ggcgacacgg aaatgttgaa tactcatatt cttccttttt caatattatt
     9181 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9241 ataaacaaat aggggtcagt gttacaacca attaaccaat tctgaacatt atcgcgagcc
     9301 catttatacc tgaatatggc tcataacacc ccttgctcat gaccaaaatc ccttaacgtg
     9361 agttacgcgc gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9421 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9481 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9541 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag cccaccactt
     9601 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9661 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9721 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9781 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9841 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9901 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9961 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    10021 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    10081 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    10141 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag gcgagagtag
    10201 ggaactgcca ggcatcaaac taagcagaag gcccctgacg gatggccttt ttgcgtttct
    10261 acaaactctt tctgtgttgt aaaacgacgg ccagtcttaa gctcgggccc cctgggcggt
    10321 tctgataacg agtaatcgtt aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt
    10381 ttatgggggg agtttaggga aagagcattt gtcagaatat ttaagggcgc ctgtcacttt
    10441 gcttgatata tgagaattat ttaaccttat aaatgagaaa aaagcaacgc actttaaata
    10501 agatacgttg ctttttcgat tgatgaacac ctataattaa actattcatc tattatttat
    10561 gattttttgt atatacaata tttctagttt gttaaagaga attaagaaaa taaatctcga
    10621 aaataataaa gggaaaatca gtttttgata tcaaaattat acatgtcaac gataatacaa
    10681 aatataatac aaactataag atgttatcag tatttattat catttagaat aaattttgtg
    10741 tcgcccttaa ttgtgagcgg ataacaatta cgagcttcat gcacagtggc gttgactcaa
    10801 tattggccat tagccatatt attcattggt tatatagcat aaatcaatat tggctattgg
    10861 ccattgcata cgttgtatct atatcataat atgtacattt atattggctc atgtccaata
    10921 tgaccgccat gtt
//
