LOCUS       sequence-386078-       13060 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13060)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 13060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-SDK1(Trunc1)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..13060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6899..6934
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6944..6967
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8627..8671
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8672..8698
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(8838..8855)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8844..8955
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9073..9093)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9078..9407
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9258..9393
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9320..9339
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9455..9502
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9521..10120
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9521..9540)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10017..10037
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10250..10371
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10287..10306)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10341..10360
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(10424..11215,11216..11284))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11047..11066
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11285..11376)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11418..11437
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     11443..11462
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      11548..12136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12037..12056
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12290..12307
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     12405..12422
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12406..12422
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12878..12894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg ccggacctgg tagacctaga ggatctcctc caggcagagc
     1021 cagaccttct ttggctccta gacctggacc tgagcctagc agaccaagag ccgctcctga
     1081 aacatctggc ggagatacag ctggcgctgg aagatgtggt ggaagaaggg ccgctaaact
     1141 cggccctggt agaagaggat ggtgggctct gcttgccctg cagcttcatc tgctgagagc
     1201 cctggctcag gatgacgtgg ccccttactt taagacagag cccggactgc cccagatcca
     1261 cctggaaggc aatagactgg tgctgacctg tctggccgaa ggctcttggc ctctggaatt
     1321 caagtggatg agggacgaca gcgagctgac cacctacagc agcgagtaca agtacatcat
     1381 ccccagcctg cagaagctgg acgccggctt ttacagatgc gtcgtgcgga atagaatggg
     1441 cgccctgctg cagagaaagt ctgaggtgca ggtcgcctac atgggcagct ttatggacac
     1501 cgaccagaga aagaccgtgt ctcagggcag agccgccatc ctgaatctgc tgcctatcac
     1561 aagctacccc agacctcaag tgacctggtt cagagagggc cacaagatca tccctagcaa
     1621 ccggatcgcc atcacactgg aaaaccagct cgtgatcctg gccaccacaa catctgatgc
     1681 tggcgcctat tacgtgcagg ccgtgaacga gaagaacggc gagaacaaga caagcccctt
     1741 catccacctg tctatcgcca gagatgtggg cacccctgaa acaatggccc ctacaattgt
     1801 ggtgcctcct ggcaatagaa gcgtggtggc cggaagctct gagacaaccc tggaatgtat
     1861 cgccagcgct agacccgtgg aagatctgag cgtgacctgg aagagaaacg gcgtgcggat
     1921 cacatctggc ctgcacagtt ttggcagacg gctgaccatc agcaacccca caagtgccga
     1981 tacaggccct tacgtgtgtg aagctgctct gccaggatct gcctttgagc ctgccagagc
     2041 caccgccttt ctgttcatca tcgagcctcc atacttcacc gccgagcctg agagcagaat
     2101 cagcgccgaa gtggaagaga cagtggatat cggctgtcag gccatgggag tgcctctgcc
     2161 tactctgcag tggtacaagg acgccatcag catcagcaga ctgcagaacc ccagatacaa
     2221 ggtgctggct tctggcggcc tgagaatcca gaagctgagg cctgaggaca gcggcatctt
     2281 tcagtgcttc gcctctaacg aaggcggcga gatccagaca cacacctacc tggacgtgac
     2341 caatatcgcc cctgtgttca cccagaggcc tgtggataca accgtgacag atggcatgac
     2401 cgccattctg agatgcgaag tgtctggcgc ccctaagcct gccatcactt ggaagagaga
     2461 gaaccacatt ctggccagcg gctccgtgcg catccctaga ttcatgctgc tggaaagcgg
     2521 cggactgcag attgccccag tgtttatcca ggatgccggc aactacacct gttacgccgc
     2581 caataccgag ggcagcctga atgcctctgc aaccctgaca gtgtggaacc ggacatccat
     2641 tgtgcaccct ccagaggacc acgtcgtgat caagggaaca accgctacac tgcactgcgg
     2701 cgccacacac gatcctagag tgtctctgag atacgtgtgg aagaaagaca acgtggccct
     2761 gacacctagc agcaccagca gaatcgtggt ggaaaaagac ggcagcctgc tgatctctca
     2821 gacttggagc ggcgacatcg gcgactactc ttgcgagatt gtgtctgaag gcggcaacga
     2881 cagccggatg gccagactgg aagtgatcga actgcctcac agccctcaga acctgctggt
     2941 gtctcctaat agcagccact ctcacgccgt ggtgctgagc tgggttcgac cttttgacgg
     3001 caacagcccc atcctgtact acatcgtgga actgtccgag aacaacagcc cttggaaagt
     3061 gcacctgagc aacgtgggac ctgagatgac aggcgtgaca gtgtctggac tgacccctgc
     3121 cagaacctac cagttcagag tgtgcgccgt gaatgaagtc ggcagaggcc agtactctgc
     3181 cgagacaagt agactgatgc tgcccgagga acctcctagc gctcctccta agaatatcgt
     3241 ggcctccggc aggaccaacc agagcattat ggttcagtgg cagcctccac ctgagacaga
     3301 gcacaatggc gtgctgagag gctacatcct gaggtacaga ctggctggac tgcctggcga
     3361 gtaccagcag agaaacatca caagccccga agtgaactac tgcctggtca ccgacctgat
     3421 catctggacc cagtatgaga tccaggtggc cgcctataat ggcgccggac tgggagtgtt
     3481 tagcagagcc gtgaccgagt atacactgca gggcgttcca accgctcctc cacagaatgt
     3541 gcagaccgaa gccgtgaaca gcaccaccat ccagtttctg tggaaccctc cacctcagca
     3601 gttcatcaac ggcatcaacc agggctataa gctgctggct tggccagccg atgctcctga
     3661 agctgtgaca gtggtcacaa tcgccccaga ctttcatggc gtgcaccacg gacacatcac
     3721 caacctgaag aagttcaccg cctacttcac cagcgtgctg tgctttacaa cacctggcga
     3781 cggccctcca tctacacctc agcttgtgtg gacccaagag gataagcctg gcgccgtggg
     3841 acacctgagc tttaccgaga ttctggacac cagcctgaag gtgtcctggc aagagcccct
     3901 ggaaaagaat ggcatcatca ccggctacca gatcagctgg gaagtgtacg gcagaaacga
     3961 ctccagactg acccacacac tgaatagcac aacccacgag tacaaaatcc agggcctgag
     4021 cagcctgacc acatacacaa ttgatgtggc cgccgtgaca gccgttggca caggactggt
     4081 cacaagcagc acaatctcta gcggcgtgcc accagatttg cctggcgctc ctagcaatct
     4141 cgtgatcagc aacatcagcc ccagaagcgc cacactgcag ttcagaccag gctacgatgg
     4201 caagaccagc atctccagat ggatcgtgga aggccaagtg ggcgccattg gagatgagga
     4261 agagtgggtc accctgtacg aggaagagaa cgagcctgac gcacagatgc tggaaatccc
     4321 caatctgacc ccttacacac actaccggtt ccggatgaag caagtgaaca tcgtgggccc
     4381 atctccatac agccccagca gcagagtgat tcagactctg caggcacctc cagatgtggc
     4441 cccaacatct gtgaccgtgc ggacagcttc cgagacatcc ctgagactga gatgggtgcc
     4501 actgccagac agccagtaca acggcaatcc tgagagcgtg ggctacagaa tcaagtactg
     4561 gcggagtgac ctgcagtcta gtgccgtggc tcaggtggtg tctgaccggc tggaaagaga
     4621 gttcaccatc gaggaactgg aagaatggat ggaatacgag ctgcagatgc aggccttcaa
     4681 tgccgttgga gctggacctt ggagcgaggt tgtgcggggc agaacaagag aaagcgtgcc
     4741 atctgccgct ccagagaatg tttctgctga ggccgtgtct agcacccaga tcctgctgac
     4801 atggacctcc gtgccagagc aggaccagaa tggactgatc ctgggctaca agatcctgtt
     4861 cagagccaag gatctcgacc ccgagcctag atctcacatc gtgcggggaa atcacaccca
     4921 gtctgctctg ctggccggac tgagaaagtt cgtgctgtat gagctgcagg tcctggcctt
     4981 taccagaatc ggcaatgggg tgccaagcac acccctgatt ctggaaagaa caaaggacga
     5041 cgcccctgga cctcctgtca gactggtttt tcctgaagtg cggctgacct ctgtgcgcat
     5101 tgtgtggcaa ccaccagagg aacccaacgg aatcatcctg ggatatcaga tcgcctaccg
     5161 gctggcctct agcagccctc acacattcac aacagtggaa gtgggagcca ccgtgcggca
     5221 gtttacagct acagatctgg cccctgagtc cgcctacatc ttcagactga gcgccaagac
     5281 tagacaaggc tggggcgaac ctctggaagc cacagtgatc accaccgaga agagagaaag
     5341 gcccgctcca cctagagagc tgctggttcc tcaggctgaa gtgaccgcca gatctctgag
     5401 actccagtgg gttccaggct ctgatggcgc ctctcctatc cggtacttta ccatgcaagt
     5461 gcgcgagctg ccaagaggcg agtggcagac atacagcagc agcatctctc acgaggccac
     5521 agcctgtgtg gtggacagac tgaggccttt caccagctac aagctgcggc tgaaggccac
     5581 caacgatatc ggcgatagcg acttcagcag cgaaaccgag gctgtgacta ccctgcagga
     5641 tgttcctggc gaaccacctg gctctgtgtc cgccacacct cacacaacaa gctccgttct
     5701 gatccaatgg cagccaccta gggacgagtc tctgaacggc ctgctccagg gatacagaat
     5761 ctactaccgc gagctggaat atgaggccgg cagcggaaca gaggctaaga ccctgaagaa
     5821 ccctatcgct ctgcacgccg aactgacagc ccagtccagc tttaagaccg tgaatagcag
     5881 ctccaccagc accatgtgcg aactgaccca tctgaagaaa tatcggcgct acgaagtgat
     5941 catgacggcc tacaacatca tcggcgagag cccagcctct gctcctgtgg aagtgtttgt
     6001 gggagaagct gcccctgcca tggctcctca gaatgtccaa gtgacacctc tgactgccag
     6061 ccagctcgaa gttacatggg acccacctcc accagaaagc cagaacggaa acattcaggg
     6121 gtacaagatc tactattggg aagccgactc tcagaacgag acagagaaga tgaaggtgct
     6181 gtttctgccc gagcctgtcg tgcgcctgaa gaatctgacc agccacacaa agtacctggt
     6241 gtctatcagc gccttcaacg ctgcaggcga cggacctaag tctgatcctc agcagggaag
     6301 aacacaccaa gcagccccag gcgctccaag ctttctggcc ttttctgaga tcaccagcac
     6361 taccctcaac gtgtcctggg gagaaccagc cgccgctaat ggcattctgc agggatatag
     6421 agtggtgtac gagcctctgg ctccagtgca aggcgtgtcc aaagtggtca ccgtggaagt
     6481 ccgcggcaat tggcagagat ggctgaaagt gcgggatctg accaagggcg tgacctactt
     6541 ctttcgggtg caagccagga ccatcacata cggaccagaa ctgcaggcca acatcacagc
     6601 cggaccagct gagggttctc ctggaagccc tagggatgtg ctggttacaa agagcgcctc
     6661 tgagctgacc ctgcaatgga cagaaggaca ctccggcgat acccctacca caggctatgt
     6721 gattgaggcc agacctagcg acgaaggact gtgggacatg ttcgtgaagg acatcccacg
     6781 cagcgccacc tcttacaccc tgagcctgga taagctgaga cagggcgtca catacgaatt
     6841 cagagtggtg gctgtgaacg aggccggcta cggcgaacct tctaatcctg gcgcgccgga
     6901 agatcaagtt gatccaagat tgattgatgg caagggcgga tctctggagg tgctgttcca
     6961 gggaccaggc ggaagcggca atcccggcgg cgatctggcc ccccagatgc tgcgcgagct
     7021 gcaggagacc aatgccgccc tgcaggacgt gcgcgagctg ctgcgccacc gcgtgaagga
     7081 gatcaccttc ctgaagaaca ccgtgatgga gtgcgacgcc tgcggtatgc agccctctag
     7141 aggctccggc tccatcatcc cagttgagga ggagaacccg gacttctgga accgcgaggc
     7201 agccgaggcc ctgggtgccg ccaagaagct gcagcctgca cagacagccg ccaagaacct
     7261 catcatcttc ctgggcgatg ggatgggggt gtctacggtg acagctgcca ggatcctaaa
     7321 agggcagaag aaggacaaac tggggcctga gatacccctg gccatggacc gcttcccata
     7381 tgtggctctg tccaagacat acaatgtaga caaacatgtg ccagacagtg gagccacagc
     7441 cacggcctac ctgtgcgggg tcaagggcaa cttccagacc attggcttga gtgcagccgc
     7501 ccgctttaac cagtgcaaca cgacacgcgg caacgaggtc atctccgtga tgaatcgggc
     7561 caagaaagca gggaagtcag tgggagtggt aaccaccaca cgagtgcagc acgcctcgcc
     7621 agccggcacc tacgcccaca cggtgaaccg caactggtac tcggacgccg acgtgcctgc
     7681 ctcggcccgc caggaggggt gccaggacat cgctacgcag ctcatctcca acatggacat
     7741 tgacgtgatc ctaggtggag gccgaaagta catgtttcgc atgggaaccc cagaccctga
     7801 gtacccagat gactacagcc aaggtgggac caggctggac gggaagaatc tggtgcagga
     7861 atggctggcg aagcgccagg gtgcccggta tgtgtggaac cgcactgagc tcatgcaggc
     7921 ttccctggac ccgtctgtga cccatctcat gggtctcttt gagcctggag acatgaaata
     7981 cgagatccac cgagactcca cactggaccc ctccctgatg gagatgacag aggctgccct
     8041 gcgcctgctg agcaggaacc cccgcggctt cttcctcttc gtggagggtg gtcgcatcga
     8101 ccatggtcat catgaaagca gggcttaccg ggcactgact gagacgatca tgttcgacga
     8161 cgccattgag agggcgggcc agctcaccag cgaggaggac acgctgagcc tcgtcactgc
     8221 cgaccactcc cacgtcttct ccttcggagg ctaccccctg cgagggagct ccatcttcgg
     8281 gctggcccct ggcaaggccc gggacaggaa ggcctacacg gtcctcctat acggaaacgg
     8341 tccaggctat gtgctcaagg acggcgcccg gccggatgtt accgagagcg agagcgggag
     8401 ccccgagtat cggcagcagt cagcagtgcc cctggacgaa gagacccacg caggcgagga
     8461 cgtggcggtg ttcgcgcgcg gcccgcaggc gcacctggtt cacggcgtgc aggagcagac
     8521 cttcatagcg cacgtcatgg ccttcgccgc ctgcctggag ccctacaccg cctgcgacct
     8581 ggcgcccccc gccggcacca ccgacgccgc gcacccgggt ggcggaggac tgaacgacat
     8641 cttcgaggct cagaaaatcg aatggcacga acatcaccat caccatcacc atcaccatta
     8701 aggccggcct gtagtcactg gcacaacgcg ttacccggta agccaatcgg gtatacacgg
     8761 tcgtcatact gcagacaggg ttcttctact ttgcaagata gtcttgagta gtaaaataaa
     8821 tagatagaga aaatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     8881 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     8941 tgaggaaatt gcatcacaac actcaaccct atctcggtct attcttttga tttataaggg
     9001 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     9061 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     9121 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     9181 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     9241 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     9301 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     9361 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     9421 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     9481 tatcggcata gtataatacg acaaggtgag gaactaaacc atgaccgagt acaagcccac
     9541 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     9601 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     9661 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     9721 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     9781 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     9841 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     9901 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     9961 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacatccg cgccccgcaa
    10021 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
    10081 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cacgtgctac gagatttcga
    10141 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
    10201 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
    10261 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    10321 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    10381 tataccgtcg acctctagct agagcttggc gtaatcatgg tcattaccaa tgcttaatca
    10441 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    10501 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagcgct gcgatgatac
    10561 cgcgagaacc acgctcaccg gctccggatt tatcagcaat aaaccagcca gccggaaggg
    10621 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    10681 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccatcgcta
    10741 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    10801 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    10861 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    10921 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    10981 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    11041 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    11101 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    11161 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    11221 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    11281 tcatattctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    11341 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggtcagtgtt acaaccaatt
    11401 aaccaattct gaacattatc gcgagcccat ttatacctga atatggctca taacacccct
    11461 tgctcatgac caaaatccct taacgtgagt tacgcgcgcg tcgttccact gagcgtcaga
    11521 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    11581 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    11641 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    11701 agtgtagccg tagttagccc accacttcaa gaactctgta gcaccgccta catacctcgc
    11761 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    11821 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    11881 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    11941 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    12001 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    12061 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    12121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    12181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    12241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    12301 gagcgaggaa gcggaaggcg agagtaggga actgccaggc atcaaactaa gcagaaggcc
    12361 cctgacggat ggcctttttg cgtttctaca aactctttct gtgttgtaaa acgacggcca
    12421 gtcttaagct cgggccccct gggcggttct gataacgagt aatcgttaat ccgcaaataa
    12481 cgtaaaaacc cgcttcggcg ggttttttta tggggggagt ttagggaaag agcatttgtc
    12541 agaatattta agggcgcctg tcactttgct tgatatatga gaattattta accttataaa
    12601 tgagaaaaaa gcaacgcact ttaaataaga tacgttgctt tttcgattga tgaacaccta
    12661 taattaaact attcatctat tatttatgat tttttgtata tacaatattt ctagtttgtt
    12721 aaagagaatt aagaaaataa atctcgaaaa taataaaggg aaaatcagtt tttgatatca
    12781 aaattataca tgtcaacgat aatacaaaat ataatacaaa ctataagatg ttatcagtat
    12841 ttattatcat ttagaataaa ttttgtgtcg cccttaattg tgagcggata acaattacga
    12901 gcttcatgca cagtggcgtt gactcaatat tggccattag ccatattatt cattggttat
    12961 atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg
    13021 tacatttata ttggctcatg tccaatatga ccgccatgtt
//
