LOCUS       sequence-386094-       12889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12889)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 12889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-SDK2(Trunc1)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..12889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6728..6763
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6773..6796
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8456..8500
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8501..8527
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(8667..8684)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8673..8784
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8902..8922)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8907..9236
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9087..9222
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9149..9168
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9284..9331
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9350..9949
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9350..9369)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9846..9866
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10079..10200
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10116..10135)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10170..10189
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(10253..11044,11045..11113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10876..10895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11114..11205)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11247..11266
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     11272..11291
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      11377..11965
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11866..11885
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12119..12136
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     12234..12251
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12235..12251
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12707..12723
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg cccaagatga tgtgtcccct tactttaaga ccgagcctgt
     1021 gcggacccag gtccaccttg agggaaatag actggtgctg acctgcatgg ccgaaggctc
     1081 ttggcctctg gaattcaagt ggctgcacaa caacagagag ctgaccaagt tcagcctgga
     1141 ataccggtac atgatcacca gcctggatag aacccacgcc ggcttctaca gatgcatcgt
     1201 gcggaataga atgggcgccc tgctgcagag acagacagag gtgcaagtgg cctacatggg
     1261 cagcttcgag gaaggcgaga aacaccagtc tgtgtctcat ggcgaggccg ccgtgatcag
     1321 agcacctaga attgccagct ttccccagcc tcaagtgacc tggttcagag atggccggaa
     1381 gatccctcct agcagcagaa tcgccatcac actggaaaac accctggtca tcctgagcac
     1441 agtggcccct gatgccggca gatattacgt gcaggccgtg aacgacaaga acggcgacaa
     1501 caagaccagc cagcctatca cactgaccgt ggaaaatgtt ggcggccctg ccgatcctat
     1561 cgctcccaca atcatcatcc ctccaaagaa cacaagcgtg gtggccggca caagcgaagt
     1621 gacactggaa tgcgtggcca acgccagacc tctgatcaag ctgcacatca tttggaagaa
     1681 agacggcgtg ctgctgagcg gcggcatcag cgatcataac agacggctga caatccccaa
     1741 tcctacaggc tccgatgccg gctactatga gtgtgaagcc gtgctgagaa gcagcagcgt
     1801 gccatctgtt gtcagaggcg cctacctgtc tgtgctggaa cctcctcagt tcgtgaaaga
     1861 gcccgagaga cacatcaccg ccgagatgga aaaggtggtg gatatccctt gtcaggccaa
     1921 gggcgtgcca ccacctagca tcacatggta caaggacgcc gccgtggtgg aagtggaaaa
     1981 gctgaccaga ttccggcaga gaaacgatgg cggcctgcag atttctggcc tggtgcctga
     2041 tgataccggc atgttccagt gcttcgccag aaatgcagcc ggcgaggtgc agacaagcac
     2101 atacctggcc gtgacctcta tcgcccctaa catcacaaga ggccctctgg acagcaccgt
     2161 gatcgatgga atgtctgtgg tgctggcctg tgaaacctct ggcgccccta gacctgccat
     2221 tacctggcag aagggcgaga gaattctggc cagtggaagc gtgcagctgc ctagattcac
     2281 acctctggaa agcggcagcc tgctgatctc ccctacacac atctctgacg ccggcaccta
     2341 cacctgtctg gccacaaata gtagaggcgt ggacgaagcc agcgccgatc tggttgtttg
     2401 ggccagaacc agaatcacca agcctccaca ggaccagagc gtgatcaagg gcacacaggc
     2461 ctctatggtt tgcggcgtga cacacgaccc tagagtgacc atccggtaca tctgggagaa
     2521 agatggggcc acactgggca ccgagtctca ccctagaatc agactggaca gaaacggctc
     2581 cctgcacatc tctcagactt ggagcggcga catcggcaca tacacatgca gagtgatcag
     2641 cgctggcggc aacgacagta gatctgccca cctgagagtc agacagctgc ctcacgctcc
     2701 tgaacaccct gtggctaccc tgtccacagt ggaaagaagg gccatcaacc tgacctggac
     2761 caagcctttc gacggcaata gccctctgat ccgctacatc ctggaaatga gcgagaacaa
     2821 cgccccttgg acagtgctgc tggcctctgt ggaccctaag gccacctctg tgacagtgaa
     2881 aggactggtg ccagccagaa gctaccagtt cagactgtgc gccgtgaatg atgtcggcaa
     2941 gggccagttc agcaaggaca ccgaaagagt gtccctgcct gaggaacctc caaccgctcc
     3001 tcctcagaat gtgatcgcca gcggcaggac caaccagagc atcatgattc agtggcagcc
     3061 acctcctgag agccaccaga atggcatcct gaagggctac atcatcagat actgcctggc
     3121 cggactgccc gtgggctatc agttcaagaa catcaccgac gccgacgtga acaacctgct
     3181 gctggaagat ctgatcatct ggaccaacta cgagatcgag gtggccgcct acaattctgc
     3241 cggcctgggc gtgtacagca gcaaagtgac agaatggaca ctgcagggcg tcccaacagt
     3301 gcctcctggc aatgtgcatg ccgaggccac caatagcacc accatcagat tcacctggaa
     3361 cgccccatct ccacagttca tcaacggcat caaccagggc tacaagctga tcgcctggga
     3421 gcctgagcaa gaggaagagg tcacaatggt caccgccagg cctaacttcc aggatagcat
     3481 ccacgtgggc ttcgtgtccg gcctgaagaa gttcaccgag tacttcacca gcgtgctgtg
     3541 cttcacaaca cctggcgacg gccctagatc tacacctcag ctcgtgcgga cacacgagga
     3601 tgttccagga cctgtgggcc acctgagctt cagcgagatt ctggatacca gcctgaaggt
     3661 gtcctggcaa gagcctggcg agaagaacgg cattctgacc ggctacagaa tcagctggga
     3721 agagtacaac cggaccaaca ccagagtgac ccactacctg cctaacgtga ccctcgagta
     3781 cagagtgaca ggactgacag ccctgaccac ctacacaatt gaagtggccg ccatgaccag
     3841 caaaggccag ggacaagtgt ctgccagcac aatctctagc ggagtgccac ctgaactgcc
     3901 cggacctcct acaaacctgg gcatcagcaa tatcggcccc agaagcgtga ccctgcagtt
     3961 tagaccaggc tacgacggca agacctccat cagcagatgg ctggtggaag cccaagtggg
     4021 agttgttgga gagggcgaag agtggctgct gatccaccag ctgagcaatg agcccgacgc
     4081 cagatccatg gaagtgcccg acctgaatcc ttttacctgc tacagcttcc ggatgcggca
     4141 agtgaacatc gtgggcacat ctccacctag ccagccaagc agaaagatcc agactctgca
     4201 ggctcctcct gacatggccc ctgctaatgt gtccctgaga acagccagcg agacaagcct
     4261 gtggctgaga tggatgcctc tgccagagat ggagtacaac ggcaatccag agagcgtggg
     4321 gtacaagatc aagtacagca gaagcgacgg ccacggaaag acactgtctc acgtggtgca
     4381 ggacagagtg gaacgggact acaccatcga ggacctggaa gagtggaccg agtatagagt
     4441 ccaggtgcag gccttcaatg ccatcggatc tggaccttgg agccagaccg ttgtgggcag
     4501 aacaagagaa tctgtgccta gcagcggccc caccaatgtg tctgctctgg ctaccacaag
     4561 cagctccatg cttgtccgtt ggagcgaagt gcctgaggcc gatagaaatg gactggtcct
     4621 gggctacaaa gtgatgtaca aagagaagga cagcgacacc cagcctcggt tttggctggt
     4681 cgagggcaat tcttcaagaa gcgctcagct gacaggcctg ggcaaatacg tgctgtatga
     4741 ggtgcaggtt ctggctttca ccagaatcgg cgacggaagc ccttctcacc ctccaattct
     4801 ggaaagaacc ctggacgacg tgccagggcc tcctatggga atcctgtttc ctgaagtgcg
     4861 gaccacctcc gtgcgactga tttggcaacc tcctgccgca cctaatggca tcatcctggc
     4921 ctaccagatc acccacagac tgaacaccac caccgccaat actgccaccg tggaagttct
     4981 ggcacctagc gccagacagt ataccgccac aggcctgaag cctgagagcg tgtacctgtt
     5041 cagaatcaca gcccagacca gaaaaggctg gggcgaagct gctgaagccc tggtggtcac
     5101 caccgagaag agagatagac ctcagcctcc aagcagaccc atggtgcaac aagaagatgt
     5161 gcgggccaga tctgtgctgc tgtcttggga acctggctct gatggcctgt ctcctgtgcg
     5221 gtactacaca atccagacaa gagagctgcc atccggcaga tgggctctgc attctgcctc
     5281 cgtgtctcac aacgcctcca gcttcatcgt ggaccggctg aagccattca ccagctacaa
     5341 gtttagagtg aaggccacaa acgatatcgg cgacagcgag ttcagcgaag agagcgagtc
     5401 tctgactacc ctgcaggcag ctcctgatga ggccccaaca attctgagcg tgacacccca
     5461 caccacaaca agcgtgctga tcagatggca gcctccagcc gaggacaaga ttaacggcat
     5521 cctgctgggc ttcagaatcc gctaccggga actgctgtac gagggcctga gaggctttac
     5581 cctgaggggc attaacaatc ctggcgccac atgggccgag ctgacctcca tgtacagcat
     5641 gagaaacctg agcaggccca gcctgacaca gtacgagctg gacaacctga acaaacatcg
     5701 gagatacgag atccggatgt ccgtgtacaa tgccgttggc gagggacctt ctagccctcc
     5761 tcaagaggtg ttcgtgggag aagctgtgcc aaccgccgca ccaagaaatg tggtggttca
     5821 tggcgctacc gccactcagc tggatgtgac atgggaacca cctccactgg acagccagaa
     5881 tggggacatc cagggctata agatctactt ctgggaagcc cagagaggga acctgaccga
     5941 gagagtgaaa accctgttcc tggccgagaa cagcgtgaag ctgaagaacc tgacaggcta
     6001 caccgcctat atggtgtccg tggctgcttt taacgcagca ggcgacggac caagaagcac
     6061 cccaacacag ggacagacac agcaagctgc ccctagcgct cctagctccg tgaagtttag
     6121 cgagctgaca acaaccagcg tcaacgtgtc ctgggaagca cctcagttcc ccaatggcat
     6181 tctggaaggc tatcggctgg tgtacgagcc ttgttctcct gtggatggcg tgtccaagat
     6241 cgtgacagtg gacgtgaagg gcaacagccc actgtggctc aaagtgaagg atctggccga
     6301 gggcgtgacc tacagattca ggatcagggc caagacattc acatacggcc ccgagattga
     6361 ggccaacgtg acaacaggac ctggcgaagg tgctccagga ccacctggcg tgccaatcat
     6421 tgtgcggtat agcagcgcca ttgccatcca ctggtcaagc ggagatcctg gcaaaggccc
     6481 catcaccaga tacgtgatcg aggccagacc tagcgacgaa ggactgtggg acatcctgat
     6541 caaggacatc cccaaagagg tgtccagcta caccttctcc atggatatcc tgaagccagg
     6601 cgtgtcctac gacttcagag tgattgctgt gaacgactac ggcttcggca cacctagcag
     6661 tccttcacag tctgtgcccg ctcagaaggc caatcctttc tacgaggaat ggtggtttgg
     6721 cgcgccggaa gatcaagttg atccaagatt gattgatggc aagggcggat ctctggaggt
     6781 gctgttccag ggaccaggcg gaagcggcaa tcccggcggc gatctggccc cccagatgct
     6841 gcgcgagctg caggagacca atgccgccct gcaggacgtg cgcgagctgc tgcgccaccg
     6901 cgtgaaggag atcaccttcc tgaagaacac cgtgatggag tgcgacgcct gcggtatgca
     6961 gccctctaga ggctccggct ccatcatccc agttgaggag gagaacccgg acttctggaa
     7021 ccgcgaggca gccgaggccc tgggtgccgc caagaagctg cagcctgcac agacagccgc
     7081 caagaacctc atcatcttcc tgggcgatgg gatgggggtg tctacggtga cagctgccag
     7141 gatcctaaaa gggcagaaga aggacaaact ggggcctgag atacccctgg ccatggaccg
     7201 cttcccatat gtggctctgt ccaagacata caatgtagac aaacatgtgc cagacagtgg
     7261 agccacagcc acggcctacc tgtgcggggt caagggcaac ttccagacca ttggcttgag
     7321 tgcagccgcc cgctttaacc agtgcaacac gacacgcggc aacgaggtca tctccgtgat
     7381 gaatcgggcc aagaaagcag ggaagtcagt gggagtggta accaccacac gagtgcagca
     7441 cgcctcgcca gccggcacct acgcccacac ggtgaaccgc aactggtact cggacgccga
     7501 cgtgcctgcc tcggcccgcc aggaggggtg ccaggacatc gctacgcagc tcatctccaa
     7561 catggacatt gacgtgatcc taggtggagg ccgaaagtac atgtttcgca tgggaacccc
     7621 agaccctgag tacccagatg actacagcca aggtgggacc aggctggacg ggaagaatct
     7681 ggtgcaggaa tggctggcga agcgccaggg tgcccggtat gtgtggaacc gcactgagct
     7741 catgcaggct tccctggacc cgtctgtgac ccatctcatg ggtctctttg agcctggaga
     7801 catgaaatac gagatccacc gagactccac actggacccc tccctgatgg agatgacaga
     7861 ggctgccctg cgcctgctga gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg
     7921 tcgcatcgac catggtcatc atgaaagcag ggcttaccgg gcactgactg agacgatcat
     7981 gttcgacgac gccattgaga gggcgggcca gctcaccagc gaggaggaca cgctgagcct
     8041 cgtcactgcc gaccactccc acgtcttctc cttcggaggc taccccctgc gagggagctc
     8101 catcttcggg ctggcccctg gcaaggcccg ggacaggaag gcctacacgg tcctcctata
     8161 cggaaacggt ccaggctatg tgctcaagga cggcgcccgg ccggatgtta ccgagagcga
     8221 gagcgggagc cccgagtatc ggcagcagtc agcagtgccc ctggacgaag agacccacgc
     8281 aggcgaggac gtggcggtgt tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca
     8341 ggagcagacc ttcatagcgc acgtcatggc cttcgccgcc tgcctggagc cctacaccgc
     8401 ctgcgacctg gcgccccccg ccggcaccac cgacgccgcg cacccgggtg gcggaggact
     8461 gaacgacatc ttcgaggctc agaaaatcga atggcacgaa catcaccatc accatcacca
     8521 tcaccattaa ggccggcctg tagtcactgg cacaacgcgt tacccggtaa gccaatcggg
     8581 tatacacggt cgtcatactg cagacagggt tcttctactt tgcaagatag tcttgagtag
     8641 taaaataaat agatagagaa aatcagcctc gactgtgcct tctagttgcc agccatctgt
     8701 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     8761 ctaataaaat gaggaaattg catcacaaca ctcaacccta tctcggtcta ttcttttgat
     8821 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     8881 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     8941 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     9001 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     9061 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     9121 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     9181 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     9241 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     9301 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tgaccgagta
     9361 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     9421 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     9481 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     9541 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     9601 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     9661 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     9721 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     9781 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacatccgc
     9841 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     9901 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac acgtgctacg
     9961 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
    10021 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
    10081 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    10141 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    10201 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt cattaccaat
    10261 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    10321 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagcgctg
    10381 cgatgatacc gcgagaacca cgctcaccgg ctccggattt atcagcaata aaccagccag
    10441 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    10501 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10561 ccatcgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10621 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10681 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10741 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    10801 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10861 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10921 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10981 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    11041 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    11101 gttgaatact catattcttc ctttttcaat attattgaag catttatcag ggttattgtc
    11161 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gtcagtgtta
    11221 caaccaatta accaattctg aacattatcg cgagcccatt tatacctgaa tatggctcat
    11281 aacacccctt gctcatgacc aaaatccctt aacgtgagtt acgcgcgcgt cgttccactg
    11341 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    11401 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    11461 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    11521 tgttcttcta gtgtagccgt agttagccca ccacttcaag aactctgtag caccgcctac
    11581 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    11641 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    11701 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    11761 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    11821 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    11881 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    11941 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    12001 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    12061 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    12121 cgagtcagtg agcgaggaag cggaaggcga gagtagggaa ctgccaggca tcaaactaag
    12181 cagaaggccc ctgacggatg gcctttttgc gtttctacaa actctttctg tgttgtaaaa
    12241 cgacggccag tcttaagctc gggccccctg ggcggttctg ataacgagta atcgttaatc
    12301 cgcaaataac gtaaaaaccc gcttcggcgg gtttttttat ggggggagtt tagggaaaga
    12361 gcatttgtca gaatatttaa gggcgcctgt cactttgctt gatatatgag aattatttaa
    12421 ccttataaat gagaaaaaag caacgcactt taaataagat acgttgcttt ttcgattgat
    12481 gaacacctat aattaaacta ttcatctatt atttatgatt ttttgtatat acaatatttc
    12541 tagtttgtta aagagaatta agaaaataaa tctcgaaaat aataaaggga aaatcagttt
    12601 ttgatatcaa aattatacat gtcaacgata atacaaaata taatacaaac tataagatgt
    12661 tatcagtatt tattatcatt tagaataaat tttgtgtcgc ccttaattgt gagcggataa
    12721 caattacgag cttcatgcac agtggcgttg actcaatatt ggccattagc catattattc
    12781 attggttata tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat
    12841 cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgtt
//
