LOCUS       sequence-386099-        3750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  promoter of the gene xylose reductase from R. toruloides for a 
            position P3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Bonturi N, Pinheiro MJ, de Oliveira PM, Rusadze E, Eichinger T, 
            Liudziute G, De Biaggi JS, Brauer A, Remm M, Miranda EA, 
            Ledesma-Amaro R, Lahtvee PJ
  TITLE     Development of a dedicated Golden Gate Assembly Platform (RtGGA) for
            Rhodotorula toruloides.
  JOURNAL   Metab Eng Commun. 2022 May 23;15:e00200. doi: 
            10.1016/j.mec.2022.e00200. eCollection 2022 Dec.
  PUBMED    35662893
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2022-05-23"; volume: "15:e00200. doi"; pages: " 
            10.1016/j.mec.2022.e00200. eCollection 2022 Dec"
COMMENT     Sequence Label: (I) pXYL (J) P3 (SBE108)
FEATURES             Location/Qualifiers
     source          1..3750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(1582..1588,1589..1606,1607..1612))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1627..1648
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1939..2527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2019..2038)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2698..3489,3490..3558))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3321..3340
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3559..3663)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3731..3749
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tatgcggtct cagtggcgac atggcgtgta ttctgcgtgc gggtaagtgt
      421 acgcgggtta gctgaagtcc tcgagaaatt agcaaaacag tgtatacgat gccacgcacc
      481 tgcacgggcg agaggaagaa ccggggacgc gaggagatga cgtgcagcgg tgcgaagcgg
      541 ggggaggacg ggcaggagtg aagcgggtca gtgagcgagc ctgggtgagt gctgtcgctc
      601 gcatgcgtcg aaagctactc acagactgag tttggctgag tctgggaggg agaaggagcg
      661 agagagagac gccagcgaag tggacaagcg tctgtcggcc cgcatggcgt cggctcgctt
      721 ccttcgtcgg acccctgtcc agcctgcgtc tcctcctcat cgatcaagca gccccagtgc
      781 actttttcag ccagcgagcg ggacagaccg ggtttcctct gcaacctcct cttcctcctc
      841 cgcccgggtt tctaccggcg cttcctgtct cttccgtctc tcctgggcgg gcagcgcacg
      901 gtccaccatt tcgagcgagc tccagcgacc cctccctgct ccaccccggc gtcggagcgt
      961 gcccctcgcg cttgagaagg ccgtcaaagt ggagggtaga atgacgacgg acgttgcaga
     1021 cgagcgtggg cgacaaagct ggcggaagaa caactctacc gcccttgttc tcctcgcgca
     1081 acgacaaacg cctgtccgcc ccgccctgct tcgtcgatgt agaatacgga catgactgag
     1141 accatgcatc tttctagaag atctcctaca atattctcag ctgccatgga aaatcgatgt
     1201 tcttctttta ttctctcaag attttcaggc tgtatattaa aacttatatt aagaactatg
     1261 ctaaccacct catcaggaac cgttgtaggt ggcgtgggtt ttcttggcaa tcgactctca
     1321 tgaaaactac gagctaaata ttcaatatgt tcctcttgac caactttatt ctgcattttt
     1381 tttgaacgag gtttagagca agcttcagga aactgagaca ggaattttat taaaaattta
     1441 aattttgaag aaagttcagg gttaatagca tccatttttt gctttgcaag ttcctcagca
     1501 ttcttaacaa aagacgtctc ttttgacatg tttaaagttt aaacctcctg tgtgaaatta
     1561 ttatccgctc ataattccac acattatacg agccggaagc ataaagtgta aagcctgggg
     1621 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgccaa ttgctttcca
     1681 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1741 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1801 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1861 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1921 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1981 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2041 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2101 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2161 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2221 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2281 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2341 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2401 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2461 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2521 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2581 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2641 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2701 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2761 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2821 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2881 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2941 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3001 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3061 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3121 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3181 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3241 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3301 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3361 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3421 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3481 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3541 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3601 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3661 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     3721 aacctataaa aataggcgta tcacgaggcc
//
