LOCUS       sequence-386101-        3257 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, 
            MF116010) for T1 position (E/F).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Bonturi N, Pinheiro MJ, de Oliveira PM, Rusadze E, Eichinger T, 
            Liudziute G, De Biaggi JS, Brauer A, Remm M, Miranda EA, 
            Ledesma-Amaro R, Lahtvee PJ
  TITLE     Development of a dedicated Golden Gate Assembly Platform (RtGGA) for
            Rhodotorula toruloides.
  JOURNAL   Metab Eng Commun. 2022 May 23;15:e00200. doi: 
            10.1016/j.mec.2022.e00200. eCollection 2022 Dec.
  PUBMED    35662893
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2022-05-23"; volume: "15:e00200. doi"; pages: " 
            10.1016/j.mec.2022.e00200. eCollection 2022 Dec"
COMMENT     Sequence Label: (E) tNOS (F) _T1 (SBE118)
FEATURES             Location/Qualifiers
     source          1..3257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(38..829,830..898))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     661..680
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(899..1003)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1071..1089
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     1395..1414
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1395..1413
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(2179..2185,2186..2203,2204..2209))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    2224..2245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2365..2382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2536..3124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2616..2635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
       61 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
      121 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
      181 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
      241 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
      301 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
      361 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
      421 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
      481 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
      541 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
      601 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
      661 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
      721 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
      781 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
      841 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
      901 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
      961 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     1021 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     1081 tcacgaggcc gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag
     1141 ctctgaagtc ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat
     1201 tagtagcatc acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa
     1261 tcttactact cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt
     1321 cattgttttt ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc
     1381 atatccatcc ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg
     1441 gctcgagttt ttcagcaaga tatgcggtct caaagccccg atctagtaac atagatgaca
     1501 ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa
     1561 tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca
     1621 tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac
     1681 cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacgt agatgagacc
     1741 atgcatcttt ctagaagatc tcctacaata ttctcagctg ccatggaaaa tcgatgttct
     1801 tcttttattc tctcaagatt ttcaggctgt atattaaaac ttatattaag aactatgcta
     1861 accacctcat caggaaccgt tgtaggtggc gtgggttttc ttggcaatcg actctcatga
     1921 aaactacgag ctaaatattc aatatgttcc tcttgaccaa ctttattctg catttttttt
     1981 gaacgaggtt tagagcaagc ttcaggaaac tgagacagga attttattaa aaatttaaat
     2041 tttgaagaaa gttcagggtt aatagcatcc attttttgct ttgcaagttc ctcagcattc
     2101 ttaacaaaag acgtctcttt tgacatgttt aaagtttaaa cctcctgtgt gaaattatta
     2161 tccgctcata attccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc
     2221 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgccaattg ctttccagtc
     2281 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2341 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2401 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2461 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2521 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2581 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2701 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2881 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2941 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3001 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3061 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3121 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3181 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3241 aaaatgaagt tttaaat
//
