LOCUS       sequence-38611-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Vick JE, Johnson ET, Choudhary S, Bloch SE, Lopez-Gallego F, 
            Srivastava P, Tikh IB, Wawrzyn GT, Schmidt-Dannert C
  TITLE     Optimized compatible set of BioBrick vectors for metabolic pathway 
            engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
  PUBMED    22033566
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2011-12"; volume: "92(6)"; pages: 
            "1275-86. Epub 2011 Oct 28"
COMMENT     Sequence Label: pUCBB-eGFP
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      132..189
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(213..230)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(464..483)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catggacgag ctgtacaagt aaatgcatcc atggcggccg cctcgagcat caccatcacc
       61 atcactgatt ctccttacgc atctgtgcgg tatttcacac cgcatgtact agttaacttc
      121 aggacctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
      181 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
      241 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      301 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      361 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
      421 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      481 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      541 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      601 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
      661 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
      721 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
      781 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
      841 tgcgctctgc tgaagccagt taccttcg
//
