LOCUS       sequence-386126-       10168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit 
            (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components 
            of a BRET2 biosensor for studying heterotrimeric G proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10168)
  AUTHORS   Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, 
            Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT
  TITLE     TRUPATH, an open-source biosensor platform for interrogating the 
            GPCR transducerome.
  JOURNAL   Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 
            10.1038/s41589-020-0535-8.
  PUBMED    32367019
REFERENCE   2  (bases 1 to 10168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 
            10.1038/s41589-020-0535-8."
COMMENT     Sequence Label: TRUPATH Triple Ga11
FEATURES             Location/Qualifiers
     source          1..10168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1937..1990
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3001..3018)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    3030..3603
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3053..3605
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3197..3214)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3424..3443
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(5759..5776)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5765..5989
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6035..6463
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6122..6141)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6332..6353
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6472..6492)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6477..6806
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6657..6792
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6719..6738
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6873..7667
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6927..6946)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7537..7556
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7841..7962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7878..7897)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7932..7951
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8011..8027)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8011..8027)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8024..8046)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8035..8051
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8059..8065,8066..8083,8084..8089))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8104..8125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8242..8259)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8413..9001)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8493..8512)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9172..9963,9964..10032))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9795..9814
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10033..10137)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgg gagagatgga gcagctgagg caggaggcag agcagctgaa
      961 gaagcagatc gccgacgcca gaaaggcctg cgcagatgtg accctggcag agctggtgag
     1021 cggactggag gtggtgggaa gggtgcagat gaggaccagg agaacactga ggggccacct
     1081 ggccaagatc tacgccatgc actgggccac agactctaag ctgctggtgt ccgcctctca
     1141 ggacggcaag ctgatcgtgt gggatagcta taccacaaac aaggtgcacg caatccccct
     1201 gcgcagctcc tgggtcatga cctgtgccta cgccccttcc ggaaatttcg tggcatgcgg
     1261 aggactggat aacatgtgct ctatctataa tctgaagagc cgggagggca acgtgaaggt
     1321 gtctagagag ctgagcgccc acaccggcta cctgtcttgc tgtcggttcc tggacgataa
     1381 caatatcgtg acatctagcg gcgacaccac atgcgcactg tgggacatcg agaccggaca
     1441 gcagaagaca gtgtttgtgg gccacacagg cgactgtatg tccctggccg tgtctccaga
     1501 tttcaatctg tttatctccg gagcatgcga cgcatctgcc aagctgtggg atgtgcggga
     1561 gggcacctgt agacagacct tcacaggcca cgagagcgac atcaacgcca tctgcttctt
     1621 tcccaatggc gaggccatct gcacaggcag cgacgatgcc tcctgtaggc tgttcgacct
     1681 gcgcgccgat caggagctga tctgcttttc tcacgagagc atcatctgtg gcatcacctc
     1741 cgtggccttt agcctgtccg gcaggctgct gttcgcagga tatgacgact tcaactgcaa
     1801 cgtgtgggac tccatgaagt ctgagagggt gggcatcctg agcggccacg ataatcgcgt
     1861 gtcctgtctg ggagtgaccg cagacggaat ggcagtggcc acaggcagct gggattcctt
     1921 tctgaagatc tggaacgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     1981 tcccggccct accggtatgg tgtccaaggg agaggagctg ttcaccggag tggtgcccat
     2041 cctggtggag ctggacggcg atgtgaatgg ccacaagttt agcgtgtccg gagagggaga
     2101 gggcgacgca acctacggca agctgacact gaagttcatc tgcaccacag gcaagctgcc
     2161 cgtgccttgg ccaaccctgg tgaccacact gtcctacggc gtgcagtgtt tttctaggta
     2221 tcccgaccac atgaagcagc acgatttctt taagtctgcc atgcctgagg gctacgtgca
     2281 ggagcggaca atcttcttta aggacgatgg caactataag accagagccg aggtgaagtt
     2341 cgagggcgac acactggtga acaggatcga gctgaagggc atcgacttta aggaggatgg
     2401 caatatcctg ggccacaagc tggagtacaa ctataatcct cacaacgtgt atatcatggc
     2461 cgataagcag aagaacggca tcaaggtcaa tttcaagatc cgccacaata tcgaggacgg
     2521 ctctgtgcag ctggccgatc actaccagca gaacacccca atcggcgacg gaccagtgct
     2581 gctgcccgat aatcactatc tgtttacaca gtctgccctg agcaaggacc ccaacgagaa
     2641 gcgggatcac atggtgctgc tggagttcgt gaccgcagca ggaatcacac tgggaatgga
     2701 cgagctgtac aagggcagcg ccggcaccat ggaggagtgg gatgtgcctc agatgaagaa
     2761 ggaggtggag agcctgaagt atcagctggc ctttcagcgg gagatggcct ccaagacaat
     2821 cccagagctg ctgaagtgga tcgaggacgg catccccaag gaccccttcc tgaatcctga
     2881 tctgatgaag aacaatccat gggtggagaa gggcaagtgc accatcctgt gaccgcggcg
     2941 tacgaggcct gcatgctccg gacctgcagg ttcgaagtcg acagatctca attggggccc
     3001 ctatagtgtc acctaaataa ttccgccccc ccctctccct cccccccccc taacgttact
     3061 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     3121 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     3181 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     3241 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     3301 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     3361 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     3421 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     3481 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     3541 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     3601 taatatgacc ctggagagca tgatggcctg ctgtctgtcc gacgaggtga aggagtctaa
     3661 gcgcatcaac gccgagatcg agaagcagct gcggagagac aagcgggatg ccaggcgcga
     3721 gctgaagctg ctgctgctgg gcaccggaga gagcggcaag tccaccttca tcaagcagat
     3781 gagaatcatc cacggcgccg gctactccga ggaggataag aggggcttca ccaagctggt
     3841 gtatcagaat atctttacag ccatgcaggc catgatcaga gccatggaga cactgaagat
     3901 cctgtacaag tatgagcaga acaaggccaa tgccctgctg atcagggagg tggacgtgga
     3961 gaaggtgacc acattcgagc accagtacgt gtctgccatc aagaccctgt gggaggaccc
     4021 cggcatccag gagtgctacg accggagaag ggagtatcag ctgtccgatt ctgccaagta
     4081 ctatctgaca gacgtggata gaatcgccac cctgggctac ctgccaacac agcaggacgt
     4141 gctgcgcgtg cgggtgccca ccacaggcat catcgagtat cctttcgatc tggagaacat
     4201 catctttcgc atggtggacg tgggaggaca gaggagcgag aggcggaagt ggattcactg
     4261 tttcgagaac gtgacctcta tcatgtttct ggtggccctg agcgagtacg atcaggtgct
     4321 ggtggagagc gacaatgagt ccggaggagg aggctctatg gcaagcaagg tgtatgatcc
     4381 tgagcagaga aagaggatga tcaccggacc acagtggtgg gcaaggtgca agcagatgaa
     4441 cgtgctggat tcctttatca attactatga ctctgagaag cacgccgaga acgccgtgat
     4501 cttcctgcac ggcaatgcca caagctccta cctgtggagg cacgtggtgc cccacatcga
     4561 gcctgtggca aggtgtatca tccccgatct gatcggcatg ggcaagtccg gcaagtctgg
     4621 caacggctcc tatagactgc tggaccacta caagtatctg accgcctggt tcgagctgct
     4681 gaatctgcct aagaagatca tctttgtggg acacgactgg ggagcagcac tggcattcca
     4741 ctacgcctat gagcaccagg ataggatcaa ggccatcgtg cacatggaga gcgtggtgga
     4801 cgtgatcgag tcctgggacg agtggcctga catcgaggag gacatcgccc tgatcaagtc
     4861 cgaggagggc gagaagatgg tgctggagaa caatttcttt gtggagaccg tgctgccttc
     4921 taagatcatg cgcaagctgg agccagagga gttcgccgcc tacctggagc cctttaagga
     4981 gaagggcgag gtgagaaggc ccacactgtc ttggccacgg gagatcccac tggtgaaggg
     5041 aggcaagcct gatgtggtgc agatcgtgag aaactacaat gcctatctga gggccagcga
     5101 cgatctgcca aagctgttta tcgagagcga ccccggcttc ttttccaacg ccatcgtgga
     5161 gggcgccaag aagttcccaa ataccgagtt tgtgaaggtg aagggcctgc actttctgca
     5221 ggaggacgcc cccgatgaga tgggcaagta catcaagagc ttcgtggagc gcgtgctgaa
     5281 gaacgagcag tctggcggcg gcggcagcaa tagaatggag gagtccaagg ccctgttcag
     5341 gaccatcatc acatatccat ggttccagaa ctctagcgtg atcctgtttc tgaataagaa
     5401 ggatctgctg gaggacaaga tcctgtactc ccacctggtg gattatttcc ctgagtttga
     5461 tggaccacag agggacgcac aggcagcccg ggagttcatc ctgaagatgt ttgtggacct
     5521 gaaccccgac tctgataaga tcatctacag ccacttcacc tgcgccaccg atacagagaa
     5581 tatccggttc gtgtttgccg ccgtgaagga cacaatcctg cagctgaacc tgaaggagta
     5641 taatctggtg tgaggtaccg agctcggatc cactagtcca gtgtggtgga attctgcaga
     5701 tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc
     5761 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     5821 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     5881 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     5941 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     6001 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     6061 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6121 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6181 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6241 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6301 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6361 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6421 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     6481 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6541 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     6601 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     6661 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6721 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     6781 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     6841 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     6901 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     6961 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     7021 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     7081 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     7141 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     7201 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     7261 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     7321 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     7381 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     7441 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     7501 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     7561 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     7621 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     7681 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     7741 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     7801 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     7861 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7921 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     7981 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8041 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8101 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8161 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8221 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8281 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8341 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8401 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8461 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8521 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8581 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8641 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8701 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8761 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8821 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8881 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8941 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9001 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9061 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9121 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9181 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9241 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9301 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9361 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9421 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9481 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9541 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9601 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9661 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9721 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9781 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9841 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9901 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9961 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10021 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10081 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10141 atttccccga aaagtgccac ctgacgtc
//
