LOCUS       sequence-386136-       14350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cassette for the deletion of ku70 and overexpression of crtE, crtI, 
            and crtYB under different combination of promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14350)
  AUTHORS   Bonturi N, Pinheiro MJ, de Oliveira PM, Rusadze E, Eichinger T, 
            Liudziute G, De Biaggi JS, Brauer A, Remm M, Miranda EA, 
            Ledesma-Amaro R, Lahtvee PJ
  TITLE     Development of a dedicated Golden Gate Assembly Platform (RtGGA) for
            Rhodotorula toruloides.
  JOURNAL   Metab Eng Commun. 2022 May 23;15:e00200. doi: 
            10.1016/j.mec.2022.e00200. eCollection 2022 Dec.
  PUBMED    35662893
REFERENCE   2  (bases 1 to 14350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2022-05-23"; volume: "15:e00200. doi"; pages: " 
            10.1016/j.mec.2022.e00200. eCollection 2022 Dec"
COMMENT     Sequence Label: ku70 insUP+
            pXYL-G418-tNOS-pXYL-crtE-tNOS-pGPD...
FEATURES             Location/Qualifiers
     source          1..14350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      8275..8527
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      11519..11770
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(12340..12359)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12341..12359)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(12470..13129)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     13044..13063
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(13130..13232)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      13333..13921
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13822..13841
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atttaggtga cactatagaa gtgtgtatcg ctcgagggat ccgaattcga agacttggta
       61 cggagccgcc tcctccacct cagcaaccaa cctaccagtc gcaccccgga tcgcagtcta
      121 gtcttccgcc gcttcccgct ctcccttctt cctcgatgcc gcctcctccg cttcctcggc
      181 ccaaggcgcc tcagagcgcc gcatcctccg cctcaacgct tccgcctccg ccattctagg
      241 cgatgccggg aggaaccgtg ctgaacggac aaggtacggc cccggcggca gtccaggcga
      301 acggggtgca gcagtgagga gcgaacagta tgcatagctg gatcgtcgca tgcgtcttag
      361 tgtcttgctt tcttgttgga aaatcgttgg aatcgttgga ccttgttgta gcctctctca
      421 cgcaacgact ggcttgtttg acgtctcttc attgcgtggg gagagtgaag cgatgtttgc
      481 aaagtaggac acaacaagcg aacggactac tgcgaacgta cacaggcgag agcgatcggg
      541 ggcgaaagtc agtcgtcgtc ctcccatcgc cgacgatgaa ggttgtccgt attctacatc
      601 gacgaagcag ggcggggcgg acaggcgttt gtcgttgcgc gaggagaaca agggcggtag
      661 agttgttctt ccgccagctt tgtcgcccac gctcgtctgc aacgtccgtc gtcattctac
      721 cctccacttt gacggccttc tcaagcgcga ggggcacgct ccgacgccgg ggtggagcag
      781 ggaggggtcg ctggagctcg ctcgaaatgg tggaccgtgc gctgcccgcc caggagagac
      841 ggaagagaca ggaagcgccg gtagaaaccc gggcggagga ggaagaggag gttgcagagg
      901 aaacccggtc tgtcccgctc gctggctgaa aaagtgcact ggggctgctt gatcgatgag
      961 gaggagacgc aggctggaca ggggtccgac gaaggaagcg agccgacgcc atgcgggccg
     1021 acagacgctt gtccacttcg ctggcgtctc tctctcgctc cttctccctc ccagactcag
     1081 ccaaactcag tctgtgagta gctttcgacg catgcgagcg acagcactca cccaggctcg
     1141 ctcactgacc cgcttcactc ctgcccgtcc tccccccgct tcgcaccgct gcacgtcatc
     1201 tcctcgcgtc cccggttctt cctctcgccc gtgcaggtgc gtggcatcgt atacactgtt
     1261 ttgctaattt ctcgaggact tcagctaacc cgcgtacact tacccgcacg cagaatacac
     1321 gccatgtcga tgggcaagga gaagacccac gtctcgcgcc cgcgcctcaa ctcgaacatg
     1381 gacgccgacc tctacggcta caagtgggcc cgcgacaacg tcggccagtc gggcgccacc
     1441 atctaccgcc tctacggcaa gccggacgcc ccggagctct tcctcaagca cggcaagggc
     1501 tcggtcgcca acgacgtcac cgacgagatg gtccgcctca actggctcac cgagttcatg
     1561 ccgctcccga ccatcaagca cttcatccgc accccggacg acgcctggct cctcaccacc
     1621 gccatcccgg gcaagaccgc cttccaggtc ctcgaggagt acccggactc gggcgagaac
     1681 atcgtcgacg ccctcgccgt cttcctccgc cgcctccact cgatcccggt ctgcaactgc
     1741 ccgttcaact cggaccgcgt cttccgcctc gcccaggccc agtcgcgcat gaacaacggc
     1801 ctcgtcgacg cctcggactt cgacgacgag cgcaacggct ggccggtcga gcaggtctgg
     1861 aaggagatgc acaagctcct cccgttctcg ccggactcgg tcgtcaccca cggcgacttc
     1921 tcgctcgaca acctcatctt cgacgagggc aagctcatcg gctgcatcga cgtcggccgc
     1981 gtcggcatcg ccgaccgcta ccaggacctc gccatcctct ggaactgcct cggcgagttc
     2041 tcgccgtcgc tccagaagcg cctcttccag aagtacggca tcgacaaccc ggacatgaac
     2101 aagctccagt tccacctcat gctcgacgag ttcttctagc gttcaaacat ttggcaataa
     2161 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     2221 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     2281 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     2341 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggtgtccgta
     2401 ttctacatcg acgaagcagg gcggggcgga caggcgtttg tcgttgcgcg aggagaacaa
     2461 gggcggtaga gttgttcttc cgccagcttt gtcgcccacg ctcgtctgca acgtccgtcg
     2521 tcattctacc ctccactttg acggccttct caagcgcgag gggcacgctc cgacgccggg
     2581 gtggagcagg gaggggtcgc tggagctcgc tcgaaatggt ggaccgtgcg ctgcccgccc
     2641 aggagagacg gaagagacag gaagcgccgg tagaaacccg ggcggaggag gaagaggagg
     2701 ttgcagagga aacccggtct gtcccgctcg ctggctgaaa aagtgcactg gggctgcttg
     2761 atcgatgagg aggagacgca ggctggacag gggtccgacg aaggaagcga gccgacgcca
     2821 tgcgggccga cagacgcttg tccacttcgc tggcgtctct ctctcgctcc ttctccctcc
     2881 cagactcagc caaactcagt ctgtgagtag ctttcgacgc atgcgagcga cagcactcac
     2941 ccaggctcgc tcactgaccc gcttcactcc tgcccgtcct ccccccgctt cgcaccgctg
     3001 cacgtcatct cctcgcgtcc ccggttcttc ctctcgcccg tgcaggtgcg tggcatcgta
     3061 tacactgttt tgctaatttc tcgaggactt cagctaaccc gcgtacactt acccgcacgc
     3121 agaatacacg ccatgtcgaa tgtcgctgga ctggtacgac aactttatcg acaaggtgca
     3181 gggcacgccg tcgtggcagc ccgcgcagga gcaggtgcgc ccgcccggca ccgccgtcgc
     3241 ctccccacat cctcgcgagt cgtcgaagcc agcgctgaca cgctcgtgtg caggtcctga
     3301 cagagccgta cacctacctc gcctcgatcc cgggcaagga agtgcgctcg gcgctcatcg
     3361 cggcgttcaa ccagtggatg ggtgtcgcag acgtcgatct cgagattgtc aagaaagtcg
     3421 tcgggatgct gcacacggcc agcctgctgt gcgctctcga gacactctcg ctgtccagca
     3481 ataccgggtg ctcacgatcc gcgtcgattc ccgcaggatg gacgacgtcg aggacgactc
     3541 gcacctccgt cgaggcatgc ctggtcagtc tccgatctcc gagccgcctc tcgtcgcaga
     3601 gactgatccc gtgcgccatc ctccagtcgc acacaagatc tacgggattc cccagacgat
     3661 caactcggcc aactatgtct actttctcgc gtttcaagaa ctccagcgga tacacccgcg
     3721 gccgggcatc aaggtcgaag agatggtcac cggtgggttc gcctcaacct tgtcgaagcc
     3781 ttggagactg acgcgatccc cgcagaggag ctgctgaacc tgcatcgcgg ccaagggatg
     3841 gacctcttct ggcgcgagaa cttgatctgc ccgacagaac cggagtacat cgacatggtc
     3901 aacaacagtg cgtcgtgcga gtctattcta tacggcgtgc gctgactcgg tcgggatcgc
     3961 agagacggga ggactgttcc gcatcgcgat caagttgatg atggccgctt cgcctgcagc
     4021 accacggtgc gctggcttaa ttccgctcgc atagattagg actgacgatt gtgctcccag
     4081 agattacgtc ccgctcgcca acctgatcgg catcatcttc cagattcgcg acgactacgt
     4141 gaacctgcaa tcggtcgagg tgcgttttcc gctcgctccg agctgcccaa agttgctcat
     4201 acatcattct tacagtacgc aaacaacaag ggcttctgcg aagacttctc cgaaggcaaa
     4261 ttctccttcc ccatcgtcca ctcgatccgc tccgacacct cgaaccgcca aatcctcaac
     4321 atcctccgcg agcggccctc ttctcccggt ccgaaagagt acgctgtcag ctacatggag
     4381 acgcggacgg gctccttcgc gtacacgcgc gaggtgttgc gcaagttgac gcagcaggcg
     4441 agggatgagg ttgcgcggct gggagggaac agaggcgtcg aggcgattct cgacaagctt
     4501 gtgttggagg aaccgcaggc gagggcgact ggcgttgagg gcgaggcgat ggagaggaag
     4561 ctggaggagg ttgtcaagag caaaccggtc aaggcggtga cgaacggcgt caatggcgtc
     4621 catgcgcacg agcttcccaa agtctgatct acgttcaaac atttggcaat aaagtttctt
     4681 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     4741 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     4801 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     4861 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cggggctttg tgactgatct
     4921 ggtgttgttc tgagcgggta gagcgagagc gggtgatggg aaagagtgca aagggacgcg
     4981 aacttgtcga tgcggcttcc ttcgctcacg aaatcagccc tcgcgcgttt cgtcccccgg
     5041 ctggacccga cctgcgctcg aggcgggcgt gtgacggctt cctgcgctgc agattcagac
     5101 ggatcatgct ctcgagggat tgtttccctc cttcaccgct tcgctcgccc tctctgcgac
     5161 gtcggctgag atcgataagt tggtgatgtg acgtggccat ctccgaggtt cctttccgac
     5221 ccttacaagc cgagattcgc gcagcttcga ttgattgtat tgctcgaggt aaccgagtat
     5281 gagggagtcg tctctcgtcg accccaagcc tgcgcatact gctgcccgct tctggtgcct
     5341 gctccaataa attactgtac ttggcgccgc gatttctgga ctatctcgct tgccttatgc
     5401 tcccgcggcg ccgctctgcg ccgttcctcc cacgacttcc atgtcctcac agcttgtttc
     5461 gtcgtccgaa gcgtcctcta cgtcttgcaa acgtcaaaaa acgacgttgg catcccggcg
     5521 gcacaaggct tgctccggta ggcgacctac aaggcgagcc agcagatttg cttgtccgca
     5581 gagatttctt ccttccatcg tgccgtctaa aggacgaaac gaggtcacgg aggagggacc
     5641 tagagagcaa ggttcattcc aatgcttgcg gagcgaggag aacgtcgaac tccaacaaat
     5701 gcgcccgctt gcacgtgaag aggagcttcg atgcgatgcc gagcaacgca aggtggagat
     5761 gatcgcttcc gggaactcat ccgctcgatc gccgatgcca ggcggggcgg tgtgatgctt
     5821 gacggggttg ctcttgtcct gccaccaact cattagcaca gcaaggatgg cagctgcgaa
     5881 cggacacggc aagggaaagc cctcggtgct catcgtcgga gcgggcgtcg gagggactgc
     5941 ctccgccgct cgcctcgccc aatccgggtt cgacgtgaca ggtgcgcaca ctctgtctcc
     6001 tgcgtcgtcc tgcctcgatc gggggacgtg ggagcactcg tagaacctgg ttggaggcac
     6061 tgacgagttc aacctcgtcg atagtcctcg agaagaacga ctttgtcggc ggacgatgct
     6121 ccctcttcac cgatccgacc aagtccttcc gcttcgacca gggcccgagc ctgttcctca
     6181 tcccgcgact gtttgacgag atctttaacg atcttgggac gagcctcgag aacgagggca
     6241 tcaggctcgt caagtgcgag ccaaactact ggatcgtctt ccccgacaag gaggtcgtcg
     6301 agatgagcag cgacttgaca agggtgaaga agcaggtcga gcggtgggag ggagagaagg
     6361 gctttgaagg gtgagtggct tggaacctcg gagcgagacg acgagcggag atgccctttc
     6421 gcttgtgctc tcttcccgct tcctttccag ttactgacgc tcatgttgta tgcagattcc
     6481 ttggcttctt gaaggaggga cacgcgcact acgagctgtc gatggtccac gtcctgcacc
     6541 gcaacttcac ctcgctcctc tcgatggtcc gcccgtctct catcatccag ctccgcaagc
     6601 tccatccctt tgtctctgtc gtacgtcacc tcatcagtgc tgatacaact cgctgggaac
     6661 gcaggattgc tgatgagcgt cgcctcgact cagtactcgc gcgcgaccaa gtacttcaag
     6721 acggaccgca tgcggagagc gttcactttc acctcgatgt atctcgtgag tcggagagcg
     6781 tcgcgattcg agcgccagct ctggctttaa tcgccggcca agttcctcct ccgctcaccc
     6841 gcacctccaa tctcgcaggg catgtccccc ttcgacgctc tcggcgccta caacctcctc
     6901 cagtacaccg agcactgcga aggcatcctc taccctctcg gcggcttcgg ccgcatcccg
     6961 caaaccctcc aacagctcgc cgaaaagagc ggcgccaagt tccgcttcaa cagtcccgtc
     7021 aagcgcgaca cggtcgagaa cggcacggct aagggtgtcg agctcgagag cggcgagaag
     7081 ttgaccgccg acatcgtcct cgtcaatgcg gatttggtgt ggagtatggc gcacctgtac
     7141 gaggagacga gctactcgaa gaggctcgag gagcggcccg tcagctgctc gtccatctcg
     7201 ttctactggc ccatgaaccg gtgcgtgaag acactctatt cctcgatcgc gaatggcgga
     7261 tccttactcc cctctggccg ctacagcaag ataccccagc tggactcgca taccatcttc
     7321 ctcgcagagg agtaccgaga gtgagcacac ctgcgctact caccgctctc gaagcggtcg
     7381 ctgacgaagc ctctcgcgtc caggtccttc gactcgatct tccgcgaaca ccgcgtcccg
     7441 catgagcctt ccttctacgt caacgttccc agccgtcacg acccgtcgta tgtcccttcc
     7501 acgtcgtcag cagccggcca tcgctgactc cgcacccgct cgcagcgccg cacccgccga
     7561 caaggacgcc gtcatcgtcc tcgttcccgt cgggcacatc tccgccgccc ccccctcctc
     7621 ttccgactgg gacaaggtcg tcgaagagac gcgcaacaag atcatcggcg agattgagcg
     7681 ccgcctcgac atcgaggacc tccggagctg catcgagcac gagacgatca acacgcccat
     7741 cacttggggc gagaagttca atttgcaccg cggcagcatc ctcggactca gtcacgactt
     7801 cttgtgagtt ccgccagcct gtcttgtcct gcttgctctt gaagcttgcg ctgaccttcc
     7861 caacctttcg cttcttcgca gcaacgtcct ctctttccgc cccaagaccc gccacccgag
     7921 tgtcaagaac gcttactttg tcggcgcgtc ggcgcacccg ggaactgggt gagttgtggt
     7981 ggtcttcgct cgccccgctt gtcgctgcct ctccagcgta ccgcatgcgt cgagttcaag
     8041 gaatggttgc taattcgtat tgtcctcgca gcgtccccat cgtcctcgcc ggcgcccgcc
     8101 tcgtcgcaac ccaaatcctc aacgacctcg ggatgcccat cccctcgcgc tggaacgtct
     8161 cctcctcgga actcgcgacg cacaggacga cccgcgatgc agcgggaggg tttacgctcc
     8221 tctcggtgtt gtttgggttg attgctttgt tggtgatgta cctgcgcggt tgaggatcgt
     8281 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     8341 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     8401 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     8461 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     8521 ctagatcggg gtcacggctg aggcttcccc gacgcccctc ctcccctccc tcctcgccct
     8581 cctcctcgtc ctcctcgtga tgccatcagt cgcgccggcg gggtgctgcg gtttgcgacg
     8641 gtgttctttg agggtgtgtg tgcgctgagc aagcagtagc aggcagtcca gcaggtgagc
     8701 gcggtgtggg aacagctcga gcttgttgcg ggcggtgtac tgatcaagta ggacctcgga
     8761 gactacggtc agagcctacg acgacgtcgt tgcagtcgtg tacactggga atggcagcta
     8821 ggctgatccc cgccgctctc caccacactt tactcgtctt ccgtcctcgc ccgcagtcgt
     8881 taattcatgg gtggagacgt cgaagggtgg gctttgcttc ggggtggctg ggtcccgtgc
     8941 tcgagtggct gtgggcgtca attaagcgtg tctcgtgcga gagcgagccg acttgcgagt
     9001 cgtcccgagc aggagtggcc ttcctcgctc ttcgccttcc ccacccatcg accgtcccgc
     9061 aaccttgcgc tcgctctctc tcttacaact tgctcttcac ttgcacacac tgctacacac
     9121 cctcgcacag cactcgcact cagcccatac cggtccagcc ttcgcagccc tctctcttcg
     9181 tgcgttccca ttcccggctg ttcactctcg caccgtcatt tcccgtctag ctgacagctg
     9241 ccacgtctcc gacagtcaca ccacatgggc ggactggact actggctcgt gtgcgtccac
     9301 ctcgctcccg ttcactcgca tcctccgcac tcaccctcgt gttgctcgca acgcccgaca
     9361 gccacctccg ctggactatc ccgccagcgc tagtcctctg gagcaccttc aggaagctca
     9421 ggacacgccg cgatgtgtac aagacgctct tccccgtcgc cgtgcgtcca caccgcttcc
     9481 tccgtcggct cgatgctcca atcgccaact gacctctgcc cgtcccacag atcgcagtaa
     9541 ccgttcgtgc agctcacaag aagcaggaga cgcgaggaac tgaccgaatc tcgcgcaggc
     9601 gacaattccc tgggactcgt acctcatccg gcacagggtg cgccaagtct tgcttcctac
     9661 cctcgaaagc gccgactaac gcttgcaccg gacagatctg gtcatacccc gagtcatccg
     9721 tcgtcggacc gaccctcttc gcgataccct acgaagagat cttcttcttc ttcgtccaga
     9781 cctacatcac cgcgaccgtc tacgccctct tcagccgccc cgtcgtgcac gccgtcctcc
     9841 tccctcggaa acctagcgac ggacgagcgg cgcggtacat cgggacggcg gcgttcttgg
     9901 gcatctttgc gctcgcgtgg gccaagttgg aagagggagg agagggaacg tacctcgcgt
     9961 tgattgttgg atgggttgcg ccgttcctcg cgttgctctg gtgcgtccaa gcgagttccc
    10021 ggcgagagtg atgactgact tgtcccttgc aggttcattg cctcgaacca cttgctcgcc
    10081 atgcctcgct gggccgtcgg tcttcccatc ctcctcccga cgctctactt gtgggagtgc
    10141 gacgctcgag cattacagcg cggaacttgg gtgatcgaga agggcacaaa gctgggcttg
    10201 gctttccgcg gccttgaaat cgagtgcgcc cggtctgcct atcatcgccc agtcgcttgc
    10261 tgacggtcaa cgttcacgca gagaggccgt cttcttcctc ttgacaaacg tcatgatcgt
    10321 cttcggcctc gtcgcctgcg actactgcct tgcagttcac gacttgcgct cctacgacac
    10381 ccgcacctcg tccgtcttcc cacccttgcg cgacttcctc cccatcctcc tcaactcgcc
    10441 cgacgccgca cagcgtcaac gcatcgagga cttgcaggcg gctatcgaca tcttgtcggt
    10501 ccattcgaag agcttctcga cggcgagtca ggtgttcgag ggcaggctga ggctggacct
    10561 tctctccctg tgagtgcggc tcgtatcgaa gcatagatca gagctgatct tcagaatacc
    10621 gcagctacgc ctggtgccga gtttgcgacg acctgatcga caacgcctcg acggtcgcgg
    10681 cggccgaatc caacatcgac atgatttcgg gctgcctcga cctcctctac cctccctcct
    10741 ccttcacgcc cacctccctc cccgtccgcg tctcgaacaa gcagatcgag gccgccttgc
    10801 ccggtttgag cgagcccgaa cgaggcactt tccgcctcct cagtcttctg ccgatcgccc
    10861 gcccaccgct cgacgagctc ctcgacggct tccgcaccga cctctccttt ctcgctctct
    10921 ccgactcgaa gggcgcgcac acgaacggta gcgcgaacgg caacgggaag tcgaacatct
    10981 ccgccgagtt gcccatcaag accgactcgg acctgctcgt ctacgccaac agcgtcgcct
    11041 cgtccgtcgc cgatctctgc gtccaactcg tctgggcgca ctgcacgcct tactcccgca
    11101 cacccgctca atcagtcccg cgcgacccga ttctgtcaga agcggagaac gcgcacgttc
    11161 tcgcggcggc gagggagatg ggacaggctc tccagctcgt caatatcgcg cgggacgtac
    11221 cggccgactt gaagattggg cggatctacc tcccaggtcg cgcgctcgac acgccggtgc
    11281 ccgagttgac gagcgacagg cgggcgttgc tctctcgtgc gaacgagatg gctgcgcata
    11341 gcaaggatgc gatagagaag ttgccgcaag aggcgagagg agggatcagg gcggcgtgtt
    11401 tggtctatct cagcatcggg gacgcggtcg ggaaggcgtt ggacgagggg agggtgatgg
    11461 agcgcgcgag ggtgtccaag ggtgcgaggg cgcgcaaagc gtggcaggcg ctgtgagtat
    11521 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
    11581 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
    11641 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
    11701 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
    11761 ttactagatc gggatgtggc gaccgggcgc gcgtttcttg ccctcggcgc aagtgggcat
    11821 tcgctcaggc gggcagggtt cttcggcggt agttcacgcg catcagtacg tgccgcgccc
    11881 tttcttcgcc ttacttattg tgagctgacg cactcttgca cagtaacgta caaagcgact
    11941 cggaagacga cgacggcttt gcgcacgtcc acgccaagca cgcctacgag aattttgacg
    12001 aggttgcgcc gcacgagatt gacccgtacg tgccgctgtc tctttcgaat gcatcacaag
    12061 ctcgagctga tgccggccgc aacacccagc ctccgcgatg ttgcggcttc gttcctcctc
    12121 gaaatgttcg aggaactctg gcgaattatg aagccgaacg agtatcgcgg cgacaacaaa
    12181 agcgacctcc tcgctcacat ccaccgacac tgcctcccct cgctcctcaa gcacatcccg
    12241 cctcggatcc accacaccgt cgcgccgaaa cggtctaccc attcctgtag tcttcttaat
    12301 taagacgtca gaattctcga ggcggccgca tgtgcgtctc cctatagtga gtcgtattaa
    12361 tttcgcgggc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    12421 catgagtagc accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc
    12481 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
    12541 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
    12601 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
    12661 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
    12721 ctttagggaa ataggccagg ttttcaccgt aacacgccac gtcttgcgaa tatatgtgta
    12781 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
    12841 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
    12901 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
    12961 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
    13021 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
    13081 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
    13141 tagctcctgg aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
    13201 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttgtcat
    13261 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    13321 cgaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    13381 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    13441 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    13501 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    13561 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    13621 gttgccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    13681 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    13741 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    13801 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    13861 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    13921 aaacgccagc aatgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    13981 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    14041 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagccga
    14101 aaaatcaata atcagacaac aagatgtgcg aactcgatat tttacacgac tctctttacc
    14161 aattctgccc cgaattacac ttaaaacgac tcaacagctt aacgttggct tgccacgcat
    14221 tacttgactg taaaactctc actcttaccg aacttggccg taacctgcca accaaagcga
    14281 gaacaaaaca taacatcaaa cgaatcgacc gattgttagg taatcgtcac ctgcaggaag
    14341 gtttaaacgc
//
