LOCUS       sequence-386137-       14353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cassette for the deletion of ku70 and overexpression of crtE, crtI, 
            and crtYB under different combination of promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14353)
  AUTHORS   Bonturi N, Pinheiro MJ, de Oliveira PM, Rusadze E, Eichinger T, 
            Liudziute G, De Biaggi JS, Brauer A, Remm M, Miranda EA, 
            Ledesma-Amaro R, Lahtvee PJ
  TITLE     Development of a dedicated Golden Gate Assembly Platform (RtGGA) for
            Rhodotorula toruloides.
  JOURNAL   Metab Eng Commun. 2022 May 23;15:e00200. doi: 
            10.1016/j.mec.2022.e00200. eCollection 2022 Dec.
  PUBMED    35662893
REFERENCE   2  (bases 1 to 14353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng 
            Commun."; date: "2022-05-23"; volume: "15:e00200. doi"; pages: " 
            10.1016/j.mec.2022.e00200. eCollection 2022 Dec"
COMMENT     Sequence Label: ku70 insUP+
            pXYL-G418-tNOS-pADH2-crtE-tNOS-pXY...
FEATURES             Location/Qualifiers
     source          1..14353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      8218..8470
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      11522..11773
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(12343..12362)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12344..12362)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(12473..13132)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     13047..13066
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(13133..13235)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      13336..13924
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13825..13844
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atttaggtga cactatagaa gtgtgtatcg ctcgagggat ccgaattcga agacttggta
       61 cggagccgcc tcctccacct cagcaaccaa cctaccagtc gcaccccgga tcgcagtcta
      121 gtcttccgcc gcttcccgct ctcccttctt cctcgatgcc gcctcctccg cttcctcggc
      181 ccaaggcgcc tcagagcgcc gcatcctccg cctcaacgct tccgcctccg ccattctagg
      241 cgatgccggg aggaaccgtg ctgaacggac aaggtacggc cccggcggca gtccaggcga
      301 acggggtgca gcagtgagga gcgaacagta tgcatagctg gatcgtcgca tgcgtcttag
      361 tgtcttgctt tcttgttgga aaatcgttgg aatcgttgga ccttgttgta gcctctctca
      421 cgcaacgact ggcttgtttg acgtctcttc attgcgtggg gagagtgaag cgatgtttgc
      481 aaagtaggac acaacaagcg aacggactac tgcgaacgta cacaggcgag agcgatcggg
      541 ggcgaaagtc agtcgtcgtc ctcccatcgc cgacgatgaa ggttgtccgt attctacatc
      601 gacgaagcag ggcggggcgg acaggcgttt gtcgttgcgc gaggagaaca agggcggtag
      661 agttgttctt ccgccagctt tgtcgcccac gctcgtctgc aacgtccgtc gtcattctac
      721 cctccacttt gacggccttc tcaagcgcga ggggcacgct ccgacgccgg ggtggagcag
      781 ggaggggtcg ctggagctcg ctcgaaatgg tggaccgtgc gctgcccgcc caggagagac
      841 ggaagagaca ggaagcgccg gtagaaaccc gggcggagga ggaagaggag gttgcagagg
      901 aaacccggtc tgtcccgctc gctggctgaa aaagtgcact ggggctgctt gatcgatgag
      961 gaggagacgc aggctggaca ggggtccgac gaaggaagcg agccgacgcc atgcgggccg
     1021 acagacgctt gtccacttcg ctggcgtctc tctctcgctc cttctccctc ccagactcag
     1081 ccaaactcag tctgtgagta gctttcgacg catgcgagcg acagcactca cccaggctcg
     1141 ctcactgacc cgcttcactc ctgcccgtcc tccccccgct tcgcaccgct gcacgtcatc
     1201 tcctcgcgtc cccggttctt cctctcgccc gtgcaggtgc gtggcatcgt atacactgtt
     1261 ttgctaattt ctcgaggact tcagctaacc cgcgtacact tacccgcacg cagaatacac
     1321 gccatgtcga tgggcaagga gaagacccac gtctcgcgcc cgcgcctcaa ctcgaacatg
     1381 gacgccgacc tctacggcta caagtgggcc cgcgacaacg tcggccagtc gggcgccacc
     1441 atctaccgcc tctacggcaa gccggacgcc ccggagctct tcctcaagca cggcaagggc
     1501 tcggtcgcca acgacgtcac cgacgagatg gtccgcctca actggctcac cgagttcatg
     1561 ccgctcccga ccatcaagca cttcatccgc accccggacg acgcctggct cctcaccacc
     1621 gccatcccgg gcaagaccgc cttccaggtc ctcgaggagt acccggactc gggcgagaac
     1681 atcgtcgacg ccctcgccgt cttcctccgc cgcctccact cgatcccggt ctgcaactgc
     1741 ccgttcaact cggaccgcgt cttccgcctc gcccaggccc agtcgcgcat gaacaacggc
     1801 ctcgtcgacg cctcggactt cgacgacgag cgcaacggct ggccggtcga gcaggtctgg
     1861 aaggagatgc acaagctcct cccgttctcg ccggactcgg tcgtcaccca cggcgacttc
     1921 tcgctcgaca acctcatctt cgacgagggc aagctcatcg gctgcatcga cgtcggccgc
     1981 gtcggcatcg ccgaccgcta ccaggacctc gccatcctct ggaactgcct cggcgagttc
     2041 tcgccgtcgc tccagaagcg cctcttccag aagtacggca tcgacaaccc ggacatgaac
     2101 aagctccagt tccacctcat gctcgacgag ttcttctagc gttcaaacat ttggcaataa
     2161 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     2221 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     2281 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     2341 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggtgtcggct
     2401 gaggcttccc cgacgcccct cctcccctcc ctcctcgccc tcctcctcgt cctcctcgtg
     2461 atgccatcag tcgcgccggc ggggtgctgc ggtttgcgac ggtgttcttt gagggtgtgt
     2521 gtgcgctgag caagcagtag caggcagtcc agcaggtgag cgcggtgtgg gaacagctcg
     2581 agcttgttgc gggcggtgta ctgatcaagt aggacctcgg agactacggt cagagcctac
     2641 gacgacgtcg ttgcagtcgt gtacactggg aatggcagct aggctgatcc ccgccgctct
     2701 ccaccacact ttactcgtct tccgtcctcg cccgcagtcg ttaattcatg ggtggggacg
     2761 tcgaagggtg ggctttgctt cggggtggct gggtcccgtg ctcgagtggc tgtgggcgtc
     2821 aattaagcgt gtctcgtgcg agagcgagcc gacttgcgag tcgtcccgag caggagtggc
     2881 cttcctcgct cttcgccttc cccacccatc gaccgtcccg caaccttgcg ctcgctctct
     2941 ctcttacagc ttgctcttca cttgcacaca ctgctacaca ccctcgcaca gcactcgcac
     3001 tcagcccata ccggtccagc cttcgcagcc ctctctcttc gtgcgttccc attcccggct
     3061 gttcactctc gcaccgtcat ttcccgtcta gctgacagct gccacgtctc cgacagtcac
     3121 aaatgtcgct ggactggtac gacaacttta tcgacaaggt gcagggcacg ccgtcgtggc
     3181 agcccgcgca ggagcaggtg cgcccgcccg gcaccgccgt cgcctcccca catcctcgcg
     3241 agtcgtcgaa gccagcgctg acacgctcgt gtgcaggtcc tgacagagcc gtacacctac
     3301 ctcgcctcga tcccgggcaa ggaagtgcgc tcggcgctca tcgcggcgtt caaccagtgg
     3361 atgggtgtcg cagacgtcga tctcgagatt gtcaagaaag tcgtcgggat gctgcacacg
     3421 gccagcctgc tgtgcgctct cgagacactc tcgctgtcca gcaataccgg gtgctcacga
     3481 tccgcgtcga ttcccgcagg atggacgacg tcgaggacga ctcgcacctc cgtcgaggca
     3541 tgcctggtca gtctccgatc tccgagccgc ctctcgtcgc agagactgat cccgtgcgcc
     3601 atcctccagt cgcacacaag atctacggga ttccccagac gatcaactcg gccaactatg
     3661 tctactttct cgcgtttcaa gaactccagc ggatacaccc gcggccgggc atcaaggtcg
     3721 aagagatggt caccggtggg ttcgcctcaa ccttgtcgaa gccttggaga ctgacgcgat
     3781 ccccgcagag gagctgctga acctgcatcg cggccaaggg atggacctct tctggcgcga
     3841 gaacttgatc tgcccgacag aaccggagta catcgacatg gtcaacaaca gtgcgtcgtg
     3901 cgagtctatt ctatacggcg tgcgctgact cggtcgggat cgcagagacg ggaggactgt
     3961 tccgcatcgc gatcaagttg atgatggccg cttcgcctgc agcaccacgg tgcgctggct
     4021 taattccgct cgcatagatt aggactgacg attgtgctcc cagagattac gtcccgctcg
     4081 ccaacctgat cggcatcatc ttccagattc gcgacgacta cgtgaacctg caatcggtcg
     4141 aggtgcgttt tccgctcgct ccgagctgcc caaagttgct catacatcat tcttacagta
     4201 cgcaaacaac aagggcttct gcgaagactt ctccgaaggc aaattctcct tccccatcgt
     4261 ccactcgatc cgctccgaca cctcgaaccg ccaaatcctc aacatcctcc gcgagcggcc
     4321 ctcttctccc ggtccgaaag agtacgctgt cagctacatg gagacgcgga cgggctcctt
     4381 cgcgtacacg cgcgaggtgt tgcgcaagtt gacgcagcag gcgagggatg aggttgcgcg
     4441 gctgggaggg aacagaggcg tcgaggcgat tctcgacaag cttgtgttgg aggaaccgca
     4501 ggcgagggcg actggcgttg agggcgaggc gatggagagg aagctggagg aggttgtcaa
     4561 gagcaaaccg gtcaaggcgg tgacgaacgg cgtcaatggc gtccatgcgc acgagcttcc
     4621 caaagtctga tctacgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     4681 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     4741 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     4801 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     4861 cggtgtcatc tatgttacta gatcggggct ttgtccgtat tctacatcga cgaagcaggg
     4921 cggggcggac aggcgtttgt cgttgcgcga ggagaacaag ggcggtagag ttgttcttcc
     4981 gccagctttg tcgcccacgc tcgtctgcaa cgtccgtcgt cattctaccc tccactttga
     5041 cggccttctc aagcgcgagg ggcacgctcc gacgccgggg tggagcaggg aggggtcgct
     5101 ggagctcgct cgaaatggtg gaccgtgcgc tgcccgccca ggagagacgg aagagacagg
     5161 aagcgccggt agaaacccgg gcggaggagg aagaggaggt tgcagaggaa acccggtctg
     5221 tcccgctcgc tggctgaaaa agtgcactgg ggctgcttga tcgatgagga ggagacgcag
     5281 gctggacagg ggtccgacga aggaagcgag ccgacgccat gcgggccgac agacgcttgt
     5341 ccacttcgct ggcgtctctc tctcgctcct tctccctccc agactcagcc aaactcagtc
     5401 tgtgagtagc tttcgacgca tgcgagcgac agcactcacc caggctcgct cactgacccg
     5461 cttcactcct gcccgtcctc cccccgcttc gcaccgctgc acgtcatctc ctcgcgtccc
     5521 cggttcttcc tctcgcccgt gcaggtgcgt ggcatcgtat acactgtttt gctaatttct
     5581 cgaggacttc agctaacccg cgtacactta cccgcacgca gaatacacgc catgtcgaca
     5641 aatgcgcccg cttgcacgtg aagaggagct tcgatgcgat gccgagcaac gcaaggtgga
     5701 gatgatcgct tccgggaact catccgctcg atcgccgatg ccaggcgggg cggtgtgatg
     5761 cttgacgggg ttgctcttgt cctgccacca actcattagc acagcaagga tggcagctgc
     5821 gaacggacac ggcaagggaa agccctcggt gctcatcgtc ggagcgggcg tcggagggac
     5881 tgcctccgcc gctcgcctcg cccaatccgg gttcgacgtg acaggtgcgc acactctgtc
     5941 tcctgcgtcg tcctgcctcg atcgggggac gtgggagcac tcgtagaacc tggttggagg
     6001 cactgacgag ttcaacctcg tcgatagtcc tcgagaagaa cgactttgtc ggcggacgat
     6061 gctccctctt caccgatccg accaagtcct tccgcttcga ccagggcccg agcctgttcc
     6121 tcatcccgcg actgtttgac gagatcttta acgatcttgg gacgagcctc gagaacgagg
     6181 gcatcaggct cgtcaagtgc gagccaaact actggatcgt cttccccgac aaggaggtcg
     6241 tcgagatgag cagcgacttg acaagggtga agaagcaggt cgagcggtgg gagggagaga
     6301 agggctttga agggtgagtg gcttggaacc tcggagcgag acgacgagcg gagatgccct
     6361 ttcgcttgtg ctctcttccc gcttcctttc cagttactga cgctcatgtt gtatgcagat
     6421 tccttggctt cttgaaggag ggacacgcgc actacgagct gtcgatggtc cacgtcctgc
     6481 accgcaactt cacctcgctc ctctcgatgg tccgcccgtc tctcatcatc cagctccgca
     6541 agctccatcc ctttgtctct gtcgtacgtc acctcatcag tgctgataca actcgctggg
     6601 aacgcaggat tgctgatgag cgtcgcctcg actcagtact cgcgcgcgac caagtacttc
     6661 aagacggacc gcatgcggag agcgttcact ttcacctcga tgtatctcgt gagtcggaga
     6721 gcgtcgcgat tcgagcgcca gctctggctt taatcgccgg ccaagttcct cctccgctca
     6781 cccgcacctc caatctcgca gggcatgtcc cccttcgacg ctctcggcgc ctacaacctc
     6841 ctccagtaca ccgagcactg cgaaggcatc ctctaccctc tcggcggctt cggccgcatc
     6901 ccgcaaaccc tccaacagct cgccgaaaag agcggcgcca agttccgctt caacagtccc
     6961 gtcaagcgcg acacggtcga gaacggcacg gctaagggtg tcgagctcga gagcggcgag
     7021 aagttgaccg ccgacatcgt cctcgtcaat gcggatttgg tgtggagtat ggcgcacctg
     7081 tacgaggaga cgagctactc gaagaggctc gaggagcggc ccgtcagctg ctcgtccatc
     7141 tcgttctact ggcccatgaa ccggtgcgtg aagacactct attcctcgat cgcgaatggc
     7201 ggatccttac tcccctctgg ccgctacagc aagatacccc agctggactc gcataccatc
     7261 ttcctcgcag aggagtaccg agagtgagca cacctgcgct actcaccgct ctcgaagcgg
     7321 tcgctgacga agcctctcgc gtccaggtcc ttcgactcga tcttccgcga acaccgcgtc
     7381 ccgcatgagc cttccttcta cgtcaacgtt cccagccgtc acgacccgtc gtatgtccct
     7441 tccacgtcgt cagcagccgg ccatcgctga ctccgcaccc gctcgcagcg ccgcacccgc
     7501 cgacaaggac gccgtcatcg tcctcgttcc cgtcgggcac atctccgccg cccccccctc
     7561 ctcttccgac tgggacaagg tcgtcgaaga gacgcgcaac aagatcatcg gcgagattga
     7621 gcgccgcctc gacatcgagg acctccggag ctgcatcgag cacgagacga tcaacacgcc
     7681 catcacttgg ggcgagaagt tcaatttgca ccgcggcagc atcctcggac tcagtcacga
     7741 cttcttgtga gttccgccag cctgtcttgt cctgcttgct cttgaagctt gcgctgacct
     7801 tcccaacctt tcgcttcttc gcagcaacgt cctctctttc cgccccaaga cccgccaccc
     7861 gagtgtcaag aacgcttact ttgtcggcgc gtcggcgcac ccgggaactg ggtgagttgt
     7921 ggtggtcttc gctcgccccg cttgtcgctg cctctccagc gtaccgcatg cgtcgagttc
     7981 aaggaatggt tgctaattcg tattgtcctc gcagcgtccc catcgtcctc gccggcgccc
     8041 gcctcgtcgc aacccaaatc ctcaacgacc tcgggatgcc catcccctcg cgctggaacg
     8101 tctcctcctc ggaactcgcg acgcacagga cgacccgcga tgcagcggga gggtttacgc
     8161 tcctctcggt gttgtttggg ttgattgctt tgttggtgat gtacctgcgc ggttgaggat
     8221 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     8281 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     8341 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     8401 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     8461 ttactagatc ggggtcatgt gactgatctg gtgttgttct gagcgggtag agcgagagcg
     8521 ggtgatggga aagagggcaa agggacgcga acttgtcgat gcggcttcct tcgctcacga
     8581 aatcagccct cgcgcgtttc gtcccccggc tggacccgac ctgcgctcga ggcgggcgtg
     8641 tgacggcttc ctgcgctgca gattcagacg gatcatgctc tcgagggatt gtttccctcc
     8701 ttcaccgctt cgctcgccct ctctgcgacg tcggctgaga tcgataagtt ggtgatgtga
     8761 cgtggccatc tccgaggttc ctttccgacc cttacaagcc gagattcgcg cagcttcgat
     8821 tgattgtatt gctcgaagta accgagtatg agggagtcgt ctctcgtcga ccccaagcct
     8881 gcgcatactg ctgcccgctt ctggtgcctg ctccaataaa ttactgtact tggcgccgcg
     8941 atttctggac tatctcgctt gccttatgcc cccgcggcgc cgctctgcgc cgttcctccc
     9001 acgacttcca tgtcctcaca gcttgtttcg tcgtccgaag cgtcctctac gtcttgcaaa
     9061 cgtcaaaaaa cgacgttggc atcccggcgg cacaaggctt gctccggtag gcgacctaca
     9121 aggcgagcca gcagatttgc ttgtccgcag agatttcttc cttccatcgt gccgtctaaa
     9181 ggacgaaacg aggtcacgga ggagggacct agagagcaag gttcattcca atgcttgcgg
     9241 agcgaggaga acgtcgaact ccaccacatg ggcggactgg actactggct cgtgtgcgtc
     9301 cacctcgctc ccgttcactc gcatcctccg cactcaccct cgtgttgctc gcaacgcccg
     9361 acagccacct ccgctggact atcccgccag cgctagtcct ctggagcacc ttcaggaagc
     9421 tcaggacacg ccgcgatgtg tacaagacgc tcttccccgt cgccgtgcgt ccacaccgct
     9481 tcctccgtcg gctcgatgct ccaatcgcca actgacctct gcccgtccca cagatcgcag
     9541 taaccgttcg tgcagctcac aagaagcagg agacgcgagg aactgaccga atctcgcgca
     9601 ggcgacaatt ccctgggact cgtacctcat ccggcacagg gtgcgccaag tcttgcttcc
     9661 taccctcgaa agcgccgact aacgcttgca ccggacagat ctggtcatac cccgagtcat
     9721 ccgtcgtcgg accgaccctc ttcgcgatac cctacgaaga gatcttcttc ttcttcgtcc
     9781 agacctacat caccgcgacc gtctacgccc tcttcagccg ccccgtcgtg cacgccgtcc
     9841 tcctccctcg gaaacctagc gacggacgag cggcgcggta catcgggacg gcggcgttct
     9901 tgggcatctt tgcgctcgcg tgggccaagt tggaagaggg aggagaggga acgtacctcg
     9961 cgttgattgt tggatgggtt gcgccgttcc tcgcgttgct ctggtgcgtc caagcgagtt
    10021 cccggcgaga gtgatgactg acttgtccct tgcaggttca ttgcctcgaa ccacttgctc
    10081 gccatgcctc gctgggccgt cggtcttccc atcctcctcc cgacgctcta cttgtgggag
    10141 tgcgacgctc gagcattaca gcgcggaact tgggtgatcg agaagggcac aaagctgggc
    10201 ttggctttcc gcggccttga aatcgagtgc gcccggtctg cctatcatcg cccagtcgct
    10261 tgctgacggt caacgttcac gcagagaggc cgtcttcttc ctcttgacaa acgtcatgat
    10321 cgtcttcggc ctcgtcgcct gcgactactg ccttgcagtt cacgacttgc gctcctacga
    10381 cacccgcacc tcgtccgtct tcccaccctt gcgcgacttc ctccccatcc tcctcaactc
    10441 gcccgacgcc gcacagcgtc aacgcatcga ggacttgcag gcggctatcg acatcttgtc
    10501 ggtccattcg aagagcttct cgacggcgag tcaggtgttc gagggcaggc tgaggctgga
    10561 ccttctctcc ctgtgagtgc ggctcgtatc gaagcataga tcagagctga tcttcagaat
    10621 accgcagcta cgcctggtgc cgagtttgcg acgacctgat cgacaacgcc tcgacggtcg
    10681 cggcggccga atccaacatc gacatgattt cgggctgcct cgacctcctc taccctccct
    10741 cctccttcac gcccacctcc ctccccgtcc gcgtctcgaa caagcagatc gaggccgcct
    10801 tgcccggttt gagcgagccc gaacgaggca ctttccgcct cctcagtctt ctgccgatcg
    10861 cccgcccacc gctcgacgag ctcctcgacg gcttccgcac cgacctctcc tttctcgctc
    10921 tctccgactc gaagggcgcg cacacgaacg gtagcgcgaa cggcaacggg aagtcgaaca
    10981 tctccgccga gttgcccatc aagaccgact cggacctgct cgtctacgcc aacagcgtcg
    11041 cctcgtccgt cgccgatctc tgcgtccaac tcgtctgggc gcactgcacg ccttactccc
    11101 gcacacccgc tcaatcagtc ccgcgcgacc cgattctgtc agaagcggag aacgcgcacg
    11161 ttctcgcggc ggcgagggag atgggacagg ctctccagct cgtcaatatc gcgcgggacg
    11221 taccggccga cttgaagatt gggcggatct acctcccagg tcgcgcgctc gacacgccgg
    11281 tgcccgagtt gacgagcgac aggcgggcgt tgctctctcg tgcgaacgag atggctgcgc
    11341 atagcaagga tgcgatagag aagttgccgc aagaggctag aggagggatc agggcggcgt
    11401 gtttggtcta tctcagcatc ggggacgcgg tcgggaaggc gttggacgag gggagggtga
    11461 tggagcgcgc gagggtgtcc aagggtgcga gggcgcgcaa agcgtggcag gcgctgtgag
    11521 tatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
    11581 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
    11641 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
    11701 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
    11761 atgttactag atcgggatgt ggcaccgggc gcgcgtttct tgccctcggc gcaagtgggc
    11821 attcgctcag gcgggcaggg ttcttcggcg gtagttcacg cgcatcagta cgtgccgcgc
    11881 cctttcttcg ccttacttat tgtgagctga cgcactcttg cacagtaacg tacaaagcga
    11941 ctcggaagac gacgacggct ttgcgcacgt ccacgccaag cacgcctacg agaattttga
    12001 cgaggttgcg ccgcacgaga ttgacccgta cgtgccgctg tctctttcga atgcatcaca
    12061 agctcgagct gatgccggcc gcaacaccca gcctccgcga tgttgcggct tcgttcctcc
    12121 tcgaaatgtt cgaggaactc tggcgaatta tgaagccgaa cgagtatcgc ggcgacaaca
    12181 aaagcgacct cctcgctcac atccaccgac actgcctccc ctcgctcctc aagcacatcc
    12241 cgcctcggat ccaccacacc gtcgcgcccg aaacggtcta cccattcctg tagtcttctt
    12301 aattaagacg tcagaattct cgaggcggcc gcatgtgcgt ctccctatag tgagtcgtat
    12361 taatttcgcg ggcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    12421 gctcatgagt agcaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc
    12481 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
    12541 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
    12601 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
    12661 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
    12721 accctttagg gaaataggcc aggttttcac cgtaacacgc cacgtcttgc gaatatatgt
    12781 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
    12841 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
    12901 tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
    12961 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
    13021 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
    13081 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
    13141 ccttagctcc tggaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
    13201 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgt
    13261 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    13321 gatcgaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    13381 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    13441 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    13501 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    13561 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    13621 atagttgccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    13681 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    13741 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    13801 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    13861 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    13921 gaaaaacgcc agcaatgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    13981 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    14041 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    14101 cgaaaaatca ataatcagac aacaagatgt gcgaactcga tattttacac gactctcttt
    14161 accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg
    14221 cattacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag
    14281 cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctgcagg
    14341 aaggtttaaa cgc
//
