LOCUS       sequence-386141-        4756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses left-side TALE–DddAtox N term half tl1-FusXTBE in 
            zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Sabharwal A, Kar B, Restrepo-Castillo S, Holmberg SR, Mathew ND, 
            Kendall BL, Cotter RP, WareJoncas Z, Seiler C, Nakamaru-Ogiso E, 
            Clark KJ, Ekker SC
  TITLE     The FusX TALE Base Editor (FusXTBE) for Rapid Mitochondrial DNA 
            Programming of Human Cells In Vitro and Zebrafish Disease Models In 
            Vivo.
  JOURNAL   CRISPR J. 2021 Dec;4(6):799-821. doi: 10.1089/crispr.2021.0061. Epub
            2021 Nov 30.
  PUBMED    34847747
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2021.0061"; journalName: "CRISPR J"; date: 
            "2021-12"; volume: "4"; issue: "6"; pages: "799-821"
COMMENT     Sequence Label: tl1-FusXTBE-RightTALE
FEATURES             Location/Qualifiers
     source          1..4756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..21
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     5'UTR           30..72
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             2424..2675
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     3'UTR           2723..2847
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     primer_bind     complement(2993..3012)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3004..3813
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3071..3090)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3956..4544
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4445..4464
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4698..4715
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaattaacc ctcactaaag ggaagcttgc ttgttctttt tgcagaagct cagaataaac
       61 gctcaacttt ggcagatctc accatggcag gttatttgaa ggtcctaagc tctctcagca
      121 ggtctgctgc cactctatcc aaaagccccg ccgttcttgc tccagcttgc cggagtttgc
      181 aacagaggaa ctatgccgat aaacgtatcc agtctaggga cgtcactaga gtggatctac
      241 gcacgctcgg ctacagtcag cagcagcaag agaagatcaa accgaaggtg cgttcgacag
      301 tggcgcagca ccacgaggca ctggtgggcc atgggtttac acacgcgcac atcgttgcgc
      361 tcagccaaca cccggcagcg ttagggaccg tcgctgtcac gtatcagcac ataatcacgg
      421 cgttgccaga ggcgacacac gaagacatcg ttggcgtcgg caaacagtgg tccggcgcac
      481 gcgccctgga ggccttgctc acggatgcgg gggagttgag aggtccgccg ttacagttgg
      541 acacaggcca acttgtgaag attgcaaaac gtggcggcgt gaccgcaatg gaggcagtgc
      601 atgcatcgcg caatgcactg acgggtgccc ccctgaacct gaccccggac caagtggtgg
      661 ctatcgccag caacggtggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
      721 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacattg
      781 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
      841 gcctgacccc ggaccaagtg gtggctatcg ccagcaacaa tggcggcaag caagcgctcg
      901 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag
      961 tggtggctat cgccagcaac aatggcggca agcaagcgct cgaaacggtg cagcggctgt
     1021 tgccggtgct gtgccaggac catggcctga cgccggacca agtggtggct atcgccagcc
     1081 acgatggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg
     1141 accatggcct gactccggac caagtggtgg ctatcgccag ccacgatggc ggcaagcaag
     1201 cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg
     1261 accaagtggt ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa acggtgcagc
     1321 ggctgttgcc ggtgctgtgc caggaccatg gcctcacacc cgatcaggtc gtggctatcg
     1381 ccagcaacgg tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
     1441 gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac ggtggcggca
     1501 agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga
     1561 ccccggacca agtggtggct atcgccagca acggtggcgg caagcaagcg ctcgaaacgg
     1621 tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg
     1681 ctatcgccag caacaatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
     1741 tgctgtgcca ggaccatggc ctgactccgg accaagtggt ggctatcgcc agccacgatg
     1801 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
     1861 gcctgacccc ggaccaagtg gtggctatcg ccagcaacat tggcggcaag caagcgctcg
     1921 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag
     1981 tggtggctat cgccagcaac attggcggca agcaagcgct cgaaacggtg cagcggctgt
     2041 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca
     2101 acggtggcgg caagcaagcg ctcgaaagca ttgtggccca gctgagccgg cctgatccgg
     2161 cgttggccgc gttgaccaac gaccacctcg tcgccttggc ctgcctcggc ggacgtcctg
     2221 ccatggatgc agtgaaaaag ggattgccgc acgcgccgga attgatcaga agagtcaatc
     2281 gccgtattgg cgaacgcacg tcccatcgcg ttgcctctag agccattcca gtgaagcgcg
     2341 gcgctaccgg tgaaaccaaa gtgtttaccg gtaacagcaa cagcccgaag agcccgacca
     2401 aaggcggttg ctctggcggc tcaactaatc tgagcgacat cattgagaag gagactggga
     2461 aacagctggt cattcaggag tccatcctga tgctgcctga ggaggtggag gaagtgatcg
     2521 gcaacaagcc agagtctgac atcctggtgc acaccgccta cgacgagtcc acagatgaga
     2581 atgtgatgct gctgacctct gacgcccccg agtataagcc ttgggccctg gtcatccagg
     2641 attctaacgg cgagaataag atcaagatgc tgtgataggc actagttagt gactgactag
     2701 gatctggcta gcgttaccac taaaccagcc tcaagaacac ccgaatggag tctctaagct
     2761 acataatacc aacttacact tacaaaatgt tgtcccccaa aatgtagcca ttcgtatctg
     2821 ctcctaataa aaagaaagtt tcttcacatt ctaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2881 accccccccc cccccccccc ccgcatgcct gcaggtcgac taggatcact agtgcggccg
     2941 cccgcggctg catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     3001 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     3061 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     3121 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     3181 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     3241 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     3301 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     3361 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     3421 aacagcgatc gcgtatttcg cctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     3481 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     3541 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     3601 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     3661 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     3721 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     3781 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     3841 tggcaaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3901 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3961 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4021 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4081 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4141 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4201 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4261 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4321 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacggttccc gaagggagaa
     4381 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4441 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4501 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4561 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4621 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4681 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaacagatc gacgatagag
     4741 cgcccaatac gcaaac
//
