LOCUS       sequence-386142-        8635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding fluorescent reporter 
            (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Lue NZ, Garcia EM, Ngan KC, Lee C, Doench JG, Liau BB
  TITLE     Base editor scanning charts the DNMT3A activity landscape.
  JOURNAL   Nat Chem Biol. 2023 Feb;19(2):176-186. doi: 
            10.1038/s41589-022-01167-4. Epub 2022 Oct 20.
  PUBMED    36266353
REFERENCE   2  (bases 1 to 8635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-022-01167-4"; journalName: "Nat Chem Biol"; date: 
            "2023-02"; volume: "19"; issue: "2"; pages: "176-186"
COMMENT     Sequence Label: pRRL-5xTetO-pEF-H2B-Citrine-SV40
FEATURES             Location/Qualifiers
     source          1..8635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1806..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2016..2034
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2052..2070
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2088..2106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2123..2141
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2159..2177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          2422..3360
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3317..3337
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        3393..3411
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3502..3879
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      3898..3907
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3904..3906,3907..3909,3910..4620)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3949..3970)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4210..4229)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4557..4578
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4672..4690)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4964..5085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             5158..5391
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5463..5584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5624..5759
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5780..5798)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5808..5825)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5808..5824)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5817..5839)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5966..6421
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6053..6072)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6263..6284
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6447..6551
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6552..6620,6621..7412)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6770..6789)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7583..8171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8072..8091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8325..8342
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8459..8480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8495..8500,8501..8518,8519..8525)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8533..8549
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8538..8560
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8557..8573
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8557..8573
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8594..8612
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     1741 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     1801 ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     1861 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     1921 tttatcgatc ctctagagat ccgatgtcga cacgcgtgca tgctacgtag cggccgcgga
     1981 tcctaggaag acgtcgatat acgcgtcgag tttactccct atcagtgata gagaacgtat
     2041 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     2101 tagagaacgt atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc
     2161 cctatcagtg atagagaacg tatgtcgagg taggcgtgta cggtgggaat gcatgagcgc
     2221 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     2281 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     2341 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     2401 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
     2461 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
     2521 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
     2581 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
     2641 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
     2701 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
     2761 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
     2821 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
     2881 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
     2941 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
     3001 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
     3061 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     3121 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     3181 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     3241 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     3301 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     3361 gtgtcgtgag ctagcgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag
     3421 ctggagctcc accgcggtgg cggccgctct agcccgggcg gatcccccgg gctgcaggaa
     3481 ttcgatatca agcttgccac catgccagag ccagcgaagt ctgctcccgc cccgaaaaag
     3541 ggctccaaga aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg caagcgcagc
     3601 cgcaaggaga gctattccat ctatgtgtac aaggttctga agcaggtcca ccctgacacc
     3661 ggcatttcgt ccaaggccat gggcatcatg aattcgtttg tgaacgacat tttcgagcgc
     3721 atcgcaggtg aggcttcccg cctggcgcat tacaacaagc gctcgaccat cacctccagg
     3781 gagatccaga cggccgtgcg cctgctgctg cctggggagt tggccaagca cgccgtgtcc
     3841 gagggtacta aggccatcac caagtacacc agcgctaagg atccccgggt accggtcgcc
     3901 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     3961 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     4021 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     4081 accctcgtga ccaccttcgg ctacggcctg atgtgcttcg cccgctaccc cgaccacatg
     4141 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     4201 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     4261 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     4321 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     4381 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     4441 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     4501 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     4561 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     4621 taggtcgacc tcgagggggg gcccggtacc aggtaagtgt acccaattcg ccctatagtg
     4681 agtcgtatta caattcactc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     4741 ggagatccaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     4801 ttagattcac agtcccaagg ctcatttcag gcccctcagt cctcacagtc tgttcatgat
     4861 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     4921 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     4981 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5041 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggta cctttaagac
     5101 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     5161 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     5221 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     5281 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     5341 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     5401 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     5461 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5521 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5581 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     5641 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5701 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5761 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     5821 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     5881 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     5941 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6001 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6061 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6121 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6181 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6241 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6301 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6361 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     6421 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6481 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6541 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6601 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6661 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6721 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6781 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6841 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6901 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6961 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7021 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7081 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7141 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7201 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7261 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7321 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7381 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7441 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7501 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8281 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8341 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8401 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     8461 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     8521 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     8581 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagc
//
