LOCUS       sequence-386151-        7005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes 
            TnsABCQ of McCAST (Tn7575)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Hsieh SC, Peters JE
  TITLE     Discovery and characterization of novel type I-D CRISPR-guided 
            transposons identified among diverse Tn7-like elements in 
            cyanobacteria.
  JOURNAL   Nucleic Acids Res. 2023 Jan 25;51(2):765-782. doi: 
            10.1093/nar/gkac1216.
  PUBMED    36537206
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-01-25- 25"; volume: "51"; issue: 
            "2"; pages: "765-782"
COMMENT     Sequence Label: pOPO636
FEATURES             Location/Qualifiers
     source          1..7005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     40..59
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(126..228)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     315..337
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     329..346
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     330..346
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    504..525
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(540..545,546..563,564..570)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    578..594
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             604..615
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      5424..5450
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(5507..5523)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5507..5523)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(5703..6248)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(5783..5802)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(6356..6450)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(6471..7005,1..125))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
       61 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
      121 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
      181 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcattga
      241 tacctgaaac aaaacccatc gtacggccaa ggaagtctcc aataactgtg atccaccaca
      301 agcgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtcatg cataatccgc
      361 acgcatctgg aataaggaag tgccattccg cctgacctgc gcccaatacg caaaccgcct
      421 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      481 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
      541 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcat
      601 gaaaaagagg agaaaggatc tatgcttgac gcaaagagtt ttgataactg gtgtcagagt
      661 cttggtgtta gtcaaacaac ccgtgccgtc attgaagggg ttcgcaactc tgctccttca
      721 cgtcgagtgc agagccgtcg gggaaatgtt gttggcaatt accctagcca caaaatgggc
      781 tttaccattc aatttgaatc acaccgcaac gagttagcgc gaatatatga gctagagcat
      841 gattccaacg ttttggagta ttacgaccaa cctataccta ttgagcttga ctacctatcc
      901 aagaatgggc ggcgtaaccg gcatccatac acaccagatt ttttcatcat ccgaaaagat
      961 tctgcgggtt gggaggaatg caagactgag caagagctta tcaagttaat agaggatagc
     1021 cccaaccgtt atcaacaaga tgagcagagc caatggcatt gtcctcccgc tgaaacctac
     1081 gcagcacgat atggcttgtt ttttaaagta cggtcagatt ccgaaataaa ctggattttt
     1141 caacaaaact tcgcttggct ggaagattat tttcacctag atagttcaga gcttagtgaa
     1201 gaagctgtgc aaaacctgct taagcaggtt tccgatgaag agggcattac gctagcagaa
     1261 ttactccggc aagctaaccc agacgatatc tataccttaa ttgcaactga ctcactgtat
     1321 gtcgatctcc atgctgctcg attagcagaa cccgatcgcg tccaagtctt ttctgatcaa
     1381 gcaactgcct ttgcttatca gcgtgctaca gaagttgctc tatcccttcc aaatcactca
     1441 caaatcattt ctataacagt tggggcttct cttgtttggg atggcgagtg ctgggaagtc
     1501 gttaatactg gaagtactac aaccggacta cttagagcag ataaccagtt cgtagagcta
     1561 ccaaatggaa ccgttgaaag tctcattcag caaggcaaaa ttactgccct aaccgaaact
     1621 gaagacacaa ctcgtcagga caaaattcaa gaaattctgc ggcgtgctaa gcctggggac
     1681 attgccgaag caaatcgaag gtatgagcaa attcagcctt atctcaaccc agatgctcca
     1741 cctacacctt ctagcaccat tcgtcgttgg cgaattcagt accgtcaagc agaggcagcc
     1801 tatggcagtg gatatgtcgg actcctacca attaattatg caaaagggaa ccgaacgagc
     1861 aaacttggtg agcctgttcg agaattcatg attaaattta tcaaggaaaa ctacgaaacc
     1921 ctgaagcatc gtagaaggtg gcgagtctac gagtctttag ttgctgcttg tgaagcccat
     1981 caacctaaac ttactcctcc tagttctaca accttctata aggagatcaa gcgccgctcc
     2041 ggcatggaac aaacggcaaa acgggaaggg cgacgggcag ctatccagga acgtcctact
     2101 tatttgcagt tggaattaac cactcctcga catggcgatc gcccctttga aattattcac
     2161 attgaccata ccgaaattga cgttgaactg gctatttcgt tgacttccct tactcaatgc
     2221 aatttcaact ccggtaattc aggtcttcct aatttaggaa gaccttgggc gacattcatg
     2281 gttgatgctt atagccgtcg tctcttagca gtctatttga gttacgagga acccagctac
     2341 cgttcctgca tgtccgtatt gcgtatctgt gttcaacgtt tcaaacgatt tcctcagtca
     2401 atcgtggtag ataatggccc agaattccac agcaactatt ttgaacaact actagcatat
     2461 tttgcttgca cgaaaaaaca ccgtcctcca gcagatgcta gatatggctt tgtcgtagag
     2521 cgtttgtttg gtactgctaa cactcaattt gttcatgagt tacgtggtaa tactcaaatt
     2581 aaacgtttgc accgcaaagt tacaaaatct gtcaaacctg agagccaggc agtttggaca
     2641 ctgggtgagt tgtatgaagc tttctgccac tgggcatatg aagtctacga taagcgacag
     2701 catccaacac taggacaaag cccccgtgaa gccttcaatg atggtttagc cattggcggt
     2761 agccgaactt gccgccgagt ggaatatgac aaaaccttcc gaattctgac tttgccagct
     2821 ccagaacgag gaaaccggaa ggtacaaccg gggcgaggca tcaaaattaa taacatttac
     2881 tattggtcga atactttccg tgacccagaa attgagcagt caaaggttga agtgcggtat
     2941 gaaccttttg atgccagtat tgcctacgca ttggtgcggg ggcagtgggt ggagtgtgtt
     3001 tctgattgct accagtatct tcagggacgt tccgaaaaag aaatccgttt ggtcagtgca
     3061 gaattagtaa aacgtaagcg agatcaagga cggaatgtga tcacctccga caaggaactg
     3121 gtgcagtttt taaactctgt tgaagccaag gaaggtaagt ttttggaaca acgcctaaaa
     3181 gcttctgaga acaagcaagt agtgcaggca attacagctg ttgaaactga ttgctctgtt
     3241 gattcagtag ttccttcgag tcctgttact gcagatggtc tatcagatag ggaaccgctg
     3301 cttgatgaac gactagagac aaatgagcct aattctgata gtagttctag caatttggaa
     3361 ttctacggag agtggtaatt atgcagttcc aaaaaggctt tccgcgagag ttattaaatc
     3421 aacccattgc tgatagagtt gcctattttc agagctatac aatgcctcat cctaagctta
     3481 aagatgccgc tcaacagttg atgctctcaa ttcatcaacc tgctggagcc tctctaatct
     3541 ttgtctttgg accaacaggg gttggcaaat ccaccttatt aaggaaggtt agaaaagaac
     3601 taattgaagc agcgttacct cggatggaaa ttgataaggg gtatattcca gttgccggaa
     3661 tcgaagcgat cgctccagaa tttagcaatt ttgattggaa agatttttat gtacgcggac
     3721 ttatggctct taatgagccg atgattgaca agaagattaa tcgtaccaat tcaaagctta
     3781 aacttcgttt ggcattagag tctgccctaa gcaatcgaaa gcctgatgca ttttttgtcg
     3841 atgaggcgca aaatttgggc aaggtggtga gcggacgcaa gctacgtgat caaactgact
     3901 gcatcaaatc cctcgcaaat attgctcaaa cccggtttgt tctttgtggc acatatgagc
     3961 ttttaatgct acggaacttg agtgctcaat tgtgcagacg tagtactgat attcatctcc
     4021 ctcgctacta tgcagaatct actcaggatc gggaaacgtt tcagggcatt gtgcaaaccc
     4081 tccagtttta ccttcccctg ccagaaactc ccgatttact gggtttatgg gagttttgtt
     4141 atgaacgtag tattggctgt gtgggcattc tgaaggactg gctatcacgc accttaaatg
     4201 ctgttctgga aaaagatcca aatgcagcta ctttaacagc tgaagatttg gaacatcatg
     4261 cttggtcaat ggagcaatgc atgattatgc tatccgaagc aaaggaagag gagcagaagt
     4321 tgacgcctag agcagaaatt cggaacgaac tgcgtgccag cctaggatta gatttacttg
     4381 cttcgtctaa agcggaaagt cccaccgaac aaaaaaaatc cagaacaaag aaaacccaag
     4441 acataggcaa acctctcccc caacgccgcc ctgttgggaa aaaccaatat gctaatccat
     4501 gaattgaagt cctacgattc atgggattta gttctaccag ctactccaga acgcagtaag
     4561 ctctactact tagaaccaat tgcggagggt actccatata ttgaagggtt agttagctat
     4621 atttgtcgat tagctaaagc gcattgtgtc tccccagcta tattaattaa gcaagaaata
     4681 ttgcctagct tcagacaaaa ctacagcata ggtttaggag cagttcatga agtcagagaa
     4741 gaggggggtc tagtttcagt ttcttctttt gcaaagccag tttataagag aaatcctaat
     4801 gaatatgggc ttttagcttg gcaatatctt gagggactca aggttttaac tctaagagag
     4861 aacctgcaac cccttacgat tccagactgg atgaataaac cttttcaagc aaagcaaagc
     4921 aaagtcgaat tggcacgaga agtccgagct tggtgtccag aatgttttca agcttggcgt
     4981 aatgctaagc aacctattta tgaacctcta ctttggtcaa ttgctgctgt tactgtttgt
     5041 cttcatcatg gtcaaccgtt gcaattctgt tgcccaaact gtaagaagac tcaacgacct
     5101 attacaggaa gaatgcaggt agggcaatgt tctggttgtt taggttggct tgatgtgcgt
     5161 ccaggtcaaa cttcagaagc ggaacatttg attgaggcag agttggattg gcacctttgg
     5221 gttgcaaagc aaatagcaga ggtactaagc gttacaccta ccattccatc gctagaaaca
     5281 aggagagcga tcgctggggt gctgagtgac aaagagaaac cctacccaat aatctcgcct
     5341 caatcaattc aacaccctca atggaacctg cttgaaacat tcgctcattg ggctcattaa
     5401 caatgccagg catcaaatta agcagaaggc catcctgacg gatggccttt aggctaggtg
     5461 gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca tagctgtttc
     5521 ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat ccgacaatct
     5581 ccaagacatt aggtggagtt cagttcggcg agcggaaatg gcttacgaac ggggcggaga
     5641 tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg
     5701 tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg
     5761 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg
     5821 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
     5881 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat
     5941 gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6001 caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg
     6061 agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact
     6121 gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa
     6181 aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac
     6241 gatctcaaga agatcatctt attaagtctg acgctctatt caacaaagcc gccgtccatg
     6301 ggtagggggc ttcaaatcgt ccccccatac gatataagtt gttactagtg cttggattct
     6361 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     6421 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
     6481 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     6541 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     6601 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     6661 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     6721 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     6781 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     6841 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     6901 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     6961 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggcc
//
