LOCUS       sequence-386163-        2879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_26.10
FEATURES             Location/Qualifiers
     source          1..2879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(283..302)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             294..1109
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1229..1774
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     1675..1694
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1891..1908
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1952..2038)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2110..2144
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             2153..2164
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa atttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
      241 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
      301 atattcagcg tgaaacgagc tgtagccgtc cgcgtctgaa cagcaacatg gatgcggatc
      361 tgtatggcta taaatgggcg cgtgataacg tgggtcagag cggcgcgacc atttatcgtc
      421 tgtatggcaa accggatgcg ccggaactgt ttctgaaaca tggcaaaggc agcgtggcga
      481 acgatgtgac cgatgaaatg gtgcgtctga actggctgac cgaatttatg ccgctgccga
      541 ccattaaaca ttttattcgc accccggatg atgcgtggct gctgaccacc gcgattccgg
      601 gcaaaaccgc gtttcaggtg ctggaagaat atccggatag cggcgaaaac attgtggatg
      661 cgctggccgt gtttctgcgt cgtctgcata gcattccggt gtgcaactgc ccgtttaaca
      721 gcgatcgtgt gtttcgtctg gcccaggcgc agagccgtat gaacaacggc ctggtggatg
      781 cgagcgattt tgatgatgaa cgtaacggct ggccggtgga acaggtgtgg aaagaaatgc
      841 ataaactgct gccgtttagc ccggatagcg tggtgaccca cggcgatttt agcctggata
      901 acctgatttt cgatgaaggc aaactgattg gctgcattga tgtgggccgt gtgggcattg
      961 cggatcgtta tcaggatctg gccattctgt ggaactgcct gggcgaattt agcccgagcc
     1021 tgcaaaaacg tctgtttcag aaatatggca ttgataatcc ggatatgaac aaactgcaat
     1081 ttcatctgat gctggatgaa tttttctaat aattaattgg accgcggtcg gctcggccca
     1141 cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtccggccgg ccctagaaat
     1201 attttatctg attaataaga tgatcttctt gagatcgttt tggtctgcgc gtaatctctt
     1261 gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc tgagctacca
     1321 actctttgaa ccgaggtaac tggcttggag gagcgcagtc accaaaactt gtcctttcag
     1381 tttagcctta accggcgcat gacttcaaga ctaactcctc taaatcaatt accagtggct
     1441 gctgccagtg gtgcttttgc atgtctttcc gggttggact caagacgata gttaccggat
     1501 aaggcgcagc ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact
     1561 gcctacccgg aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat
     1621 gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccgc cagggggaaa
     1681 cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc
     1741 gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg ccctctcact
     1801 tccctgttaa gtatcttcct ggcatcttcc aggaaatctc cgccccgttc gtaagccatt
     1861 tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga agcggaatat
     1921 atccggcgcg cccagctgtc tagggcggcg gatttgtcct actcaggaga gcgttcaccg
     1981 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
     2041 gcctttaatt aaggctcggg agacctatcg gtaataacag tccaatctgg tgtaacttcg
     2101 gaatcgtcct tgacagctag ctcagtccta ggtataatgc tagctactag tgaaagagga
     2161 gaaatactag tatggttagc aaaggcgagg cggttatcaa ggagtttatg cgttttaagg
     2221 ttcacatgga gggtagcatg aatggtcacg agttcgagat cgagggtgaa ggcgagggtc
     2281 gtccgtacga aggcacccag accgcgaagc tgaaagtgac caagggtggc ccgctgccgt
     2341 tcagctggga catcctgagc ccgcagttca tgtatggcag ccgtgcgttt accaaacacc
     2401 cggcggacat tccggattac tataagcaaa gcttcccgga aggttttaaa tgggagcgtg
     2461 ttatgaactt cgaagatggt ggcgcggtga ccgttaccca ggacaccagc ctggaggatg
     2521 gcaccctgat ttacaaggtg aaactgcgtg gcaccaactt tccgccggat ggtccggtta
     2581 tgcagaagaa aacgatgggt tgggaagcga gcaccgagcg tctgtatccg gaagatggcg
     2641 tgctgaaggg tgatatcaaa atggcgctgc gtctgaagga cggtggccgt tacctggcgg
     2701 attttaagac cacctataaa gcgaagaaac cggtgcaaat gccgggtgcg tacaacgttg
     2761 accgtaaact ggatattacc agccacaacg aggattatac cgtggttgag caatatgagc
     2821 gtagcgaggg tcgccacagc accggcggca tggacgaact gtataaggga tcctaataa
//
