LOCUS       sequence-386164-        3728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_37_pKD46.10
FEATURES             Location/Qualifiers
     source          1..3728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        210..312
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             313..972
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(379..398)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(1102..2052)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2100..2322
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2605..2691)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2855..2889
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_RNA        2892..2941
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             3007..3015
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa attggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
      241 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
      301 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      361 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      421 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      481 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
      541 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      601 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      661 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
      721 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
      781 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
      841 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
      901 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
      961 ggcggggcgt aatttgactt ttgtcggctc ggcccacttg tgtgtctcgt acaattctga
     1021 ggtccacaca gatagtgtcc tgttctactt ttgtttgtta gtcttgatgc ttcactgata
     1081 gatacaagag ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt
     1141 ggttcgttgt ttttgcgtga gccatgagaa cgaaccattg agatcatact tactttgcat
     1201 gtcactcaaa aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg
     1261 tagtgttttt cttagtccgt tacgtaggta ggaatctgat gtaatggttg ttggtatttt
     1321 gtcaccattc atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc
     1381 tagttcaact tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt
     1441 catattgctg taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct
     1501 tttaaactca tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat
     1561 ctctatattt gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat
     1621 cctcatagag tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt
     1681 ttttaactgg aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct
     1741 tgagaacttg gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct
     1801 gatttccaca gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt
     1861 ttccctactg atgttcatca tctgagcgta ttggttataa gtgaacgata ccgtccgttc
     1921 tttccttgta gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt
     1981 ggtttcatgc tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac
     2041 taattcagac atacatctca attggtctag gtgattttaa tcactatacc aattgagatg
     2101 ggctagtcaa tgataattac tagtcctttt cctttgagtt gtgggtatct gtaaattctg
     2161 ctagaccttt gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc
     2221 tttgtgtgtt ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata
     2281 aaaaaagata aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc
     2341 gcagtattac aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa
     2401 ccctaaaggc ttaagtagca ccctcgcaag ctcggttgcg gccgcaatcg ggcaaatcgc
     2461 tgaatattcc ttttgtctcc gaccatcagg cacctgagtc gctgtctttt tcgtgacatt
     2521 cagttcgctg cgctcacggc tctggcagtg aatgggggta aatggcacta caggcgcggc
     2581 gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     2641 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgccttta
     2701 attaaggctc gggagaccta tcggtaataa cagtccaatc tggtgtaact tcggaatcgt
     2761 ccccaattat tgaacaccct tcggggtgtt tttttgtttc tggtctacca tctcgttgtg
     2821 ataatagacc tgaagtgcct actctggaaa atctttgaca gctagctcag tcctaggtat
     2881 aatgctagca gctgtcaccg gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca
     2941 gcctctacaa ataattttgt ttaaggctcg ctacgagaag attgttactt tcgcagcgtt
     3001 attatctaag gaggtagtcc atggttagca aaggcgaggc ggttatcaag gagtttatgc
     3061 gttttaaggt tcacatggag ggtagcatga atggtcacga gttcgagatc gagggtgaag
     3121 gcgagggtcg tccgtacgaa ggcacccaga ccgcgaagct gaaagtgacc aagggtggcc
     3181 cgctgccgtt cagctgggac atcctgagcc cgcagttcat gtatggcagc cgtgcgttta
     3241 ccaaacaccc ggcggacatt ccggattact ataagcaaag cttcccggaa ggttttaaat
     3301 gggagcgtgt tatgaacttc gaagatggtg gcgcggtgac cgttacccag gacaccagcc
     3361 tggaggatgg caccctgatt tacaaggtga aactgcgtgg caccaacttt ccgccggatg
     3421 gtccggttat gcagaagaaa acgatgggtt gggaagcgag caccgagcgt ctgtatccgg
     3481 aagatggcgt gctgaagggt gatatcaaaa tggcgctgcg tctgaaggac ggtggccgtt
     3541 acctggcgga ttttaagacc acctataaag cgaagaaacc ggtgcaaatg ccgggtgcgt
     3601 acaacgttga ccgtaaactg gatattacca gccacaacga ggattatacc gtggttgagc
     3661 aatatgagcg tagcgagggt cgccacagca ccggcggcat ggacgaactg tataagggat
     3721 cctaataa
//
