LOCUS       sequence-386165-        3750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BASIC DNA assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Haines MC, Carling B, Marshall J, Shenshin VA, Baldwin GS, Freemont 
            P, Storch M
  TITLE     basicsynbio and the BASIC SEVA collection: software and vectors for 
            an established DNA assembly method.
  JOURNAL   Synth Biol (Oxf). 2022 Oct 11;7(1):ysac023. doi: 
            10.1093/synbio/ysac023. eCollection 2022.
  PUBMED    36381610
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-10-11"; volume: "7(1):ysac023. doi"; pages: " 
            10.1093/synbio/ysac023. eCollection 2022"
COMMENT     Sequence Label: BASIC_SEVA_67_pKD46.10
FEATURES             Location/Qualifiers
     source          1..3750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      68..162
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(193..198,199..215,216..221)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             410..943
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     CDS             complement(1124..2074)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2122..2344
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2627..2713)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        2877..2911
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     misc_RNA        2914..2963
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             3029..3037
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact
       61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat
      181 tacgatttaa atttgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg
      241 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
      301 gttttcatgg cttgttatga ctgttttttt gtacagccta tgcctcgggc atccaagcag
      361 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
      421 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaggtg gctcaagtat
      481 gggcatcatt cgcacatgta ggctcggccc tgaccaagtc aaatccatgc gggctgctct
      541 tgatcttttc ggtcgtgagt tcggagacgt agccacctac tcccaacatc agccggactc
      601 cgattacctc gggaacttgc tccgtagtaa gacattcatc gcgcttgctg ccttcgacca
      661 agaagcggtt gttggcgctc tcgcggctta cgttctgccc aagtttgagc agccgcgtag
      721 tgagatctat atctatgatc tcgcagtctc cggagagcac cggaggcagg gcattgccac
      781 cgcgctcatc aatctcctca agcatgaggc caacgcgctt ggtgcttatg tgatctacgt
      841 gcaagcagat tacggtgacg atcccgcagt ggctctctat acaaagttgg gcatacggga
      901 agaagtgatg cactttgata tcgacccaag taccgccacc taacaattcg ttcaagccga
      961 gatcggcttc ccggccgcgg agttgttcgg taaattggac aacggtcggc tcggcccact
     1021 tgtgtgtctc gtacaattct gaggtccaca cagatagtgt cctgttctac ttttgtttgt
     1081 tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt
     1141 tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat
     1201 tgagatcata cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa
     1261 tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttacgtagg taggaatctg
     1321 atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg
     1381 ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc
     1441 ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt
     1501 tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc attaacatga
     1561 acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt tgtgttagtt
     1621 cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac ttaacatgtt
     1681 ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa
     1741 aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa
     1801 agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt
     1861 tagctaatac accataagca ttttccctac tgatgttcat catctgagcg tattggttat
     1921 aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg ttgagtagtg
     1981 ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta
     2041 gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt
     2101 aatcactata ccaattgaga tgggctagtc aatgataatt actagtcctt ttcctttgag
     2161 ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat tctgctagac
     2221 cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa gtggttataa
     2281 tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc cctgtgtata
     2341 actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc
     2401 ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca agctcggttg
     2461 cggccgcaat cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag
     2521 tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg
     2581 taaatggcac tacaggcgcg gcgcgcccag ctgtctaggg cggcggattt gtcctactca
     2641 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct
     2701 ttcgttttat ttgatgcctt taattaaggc tcgggagacc tatcggtaat aacagtccaa
     2761 tctggtgtaa cttcggaatc gtccccaatt attgaacacc cttcggggtg tttttttgtt
     2821 tctggtctac catctcgttg tgataataga cctgaagtgc ctactctgga aaatctttga
     2881 cagctagctc agtcctaggt ataatgctag cagctgtcac cggatgtgct ttccggtctg
     2941 atgagtccgt gaggacgaaa cagcctctac aaataatttt gtttaaggct cgctacgaga
     3001 agattgttac tttcgcagcg ttattatcta aggaggtagt ccatggttag caaaggcgag
     3061 gcggttatca aggagtttat gcgttttaag gttcacatgg agggtagcat gaatggtcac
     3121 gagttcgaga tcgagggtga aggcgagggt cgtccgtacg aaggcaccca gaccgcgaag
     3181 ctgaaagtga ccaagggtgg cccgctgccg ttcagctggg acatcctgag cccgcagttc
     3241 atgtatggca gccgtgcgtt taccaaacac ccggcggaca ttccggatta ctataagcaa
     3301 agcttcccgg aaggttttaa atgggagcgt gttatgaact tcgaagatgg tggcgcggtg
     3361 accgttaccc aggacaccag cctggaggat ggcaccctga tttacaaggt gaaactgcgt
     3421 ggcaccaact ttccgccgga tggtccggtt atgcagaaga aaacgatggg ttgggaagcg
     3481 agcaccgagc gtctgtatcc ggaagatggc gtgctgaagg gtgatatcaa aatggcgctg
     3541 cgtctgaagg acggtggccg ttacctggcg gattttaaga ccacctataa agcgaagaaa
     3601 ccggtgcaaa tgccgggtgc gtacaacgtt gaccgtaaac tggatattac cagccacaac
     3661 gaggattata ccgtggttga gcaatatgag cgtagcgagg gtcgccacag caccggcggc
     3721 atggacgaac tgtataaggg atcctaataa
//
