LOCUS       sequence-386177-       10280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 S_D215G in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10280)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 10280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: HA-C Sars-CoV-2 S_D215G
FEATURES             Location/Qualifiers
     source          1..10280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    515..739
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    1023..1070
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1078..2100
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             1078..2100
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    2230..2351
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2267..2286)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2321..2340
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2400..2416)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2400..2416)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2413..2435)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2424..2440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2448..2454,2455..2472,2473..2478))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2493..2514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2631..2648)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2802..3390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2882..2901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3561..4352,4353..4421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4184..4203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4422..4526)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4601..4620)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4792..5171
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5172..5375
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5326..5346
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    5377..5395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5398..5416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     5426..5450
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    5593..5617
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9437..9461)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9508..9531
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             9568..9591
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             9622..9648
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             9622..9648
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    9698..9922
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gacaaagtgc gcgtgaagtg gcccaacgac ctgtatctgc aggaccggaa gctggccggc
       61 atcctggtgg aactgaccgg caaaacaggc gacgccgccc agatcgtgat cggcgctggc
      121 atcaacatgg ccatgcggcg ggtggaagag agcgtggtca accagggctg gatcaccctg
      181 caggaagccg gcatcaacct ggaccggaac accctggccg ccatgctgat cagagagctg
      241 agagccgccc tggaactgtt cgagcaggaa ggcctggccc cctacctgag cagatgggag
      301 aagctggaca acttcatcaa ccggcccgtg aaactgatca tcggcgacaa agagatcttc
      361 ggcatcagcc ggggcatcga caagcagggg gccctgctcc tggaacagga cggcatcatc
      421 aagccctgga tgggcggcga gatcagcctg cggagcgccg agaagtgatg actcgagtct
      481 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
      541 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
      601 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
      661 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
      721 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
      781 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
      841 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
      901 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
      961 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtacc
     1021 tagaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc cttggccaaa
     1081 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     1141 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     1201 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     1261 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
     1321 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     1381 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
     1441 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     1501 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     1561 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     1621 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     1681 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     1741 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     1801 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     1861 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     1921 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     1981 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     2041 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     2101 cacgtactac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     2161 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     2221 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2281 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2341 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     2401 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     2461 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     2521 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     2581 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     2641 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2701 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2761 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2821 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2881 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2941 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3001 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3061 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3121 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3181 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3241 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3301 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3361 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3421 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3481 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3541 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3601 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3661 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3721 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3781 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3841 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3901 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3961 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4021 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4081 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4141 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4201 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4261 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4321 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4381 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4441 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4501 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     4561 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     4621 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     4681 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     4741 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
     4801 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     4861 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     4921 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4981 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     5041 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     5101 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     5161 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     5221 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     5281 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     5341 ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat agagatctcc
     5401 ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc gcctggagac
     5461 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccggactc
     5521 tagcgtttaa acttaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt
     5581 ctgcagatat caacaagttt gtacaaaaaa gttggcatgt tcgtgttcct ggtgctgctg
     5641 ccactggtga gctcccagtg cgtgaacctg accaccagaa cccagctgcc acctgcctac
     5701 accaactcct tcaccagggg cgtgtactac ccagacaagg tgttcagatc cagcgtgctg
     5761 cactctaccc aggacctgtt cctgccattc ttctctaacg tgacctggtt ccacgccatc
     5821 cacgtgtctg gaaccaacgg caccaagaga ttcgacaacc cagtgctgcc attcaacgac
     5881 ggcgtgtact tcgcttctac cgagaagtct aacatcatca ggggttggat cttcggaacc
     5941 accctggact ctaagaccca gtctctgctg atcgtgaaca acgctaccaa cgtggtgatc
     6001 aaggtgtgcg agttccagtt ctgcaacgac ccattcctgg gagtgtacta ccacaagaac
     6061 aacaagtctt ggatggaaag cgagttcagg gtgtacagct ctgctaacaa ctgcaccttc
     6121 gagtacgtgt ctcagccttt cctgatggac ctggaaggaa agcagggaaa cttcaagaac
     6181 ctgagagagt tcgtgttcaa gaacatcgac ggttacttca agatatacag caagcacacc
     6241 ccaatcaacc tggtgagggr cctgccacag ggtttctctg ctctggagcc cctggtggac
     6301 ctgccaatcg gcatcaacat caccaggttc cagaccctgc tggctctgca caggtcttac
     6361 ctgaccccag gcgacagcag ctctggatgg accgccggcg ccgctgctta ctacgtggga
     6421 tacctgcagc caaggacctt cctgctgaag tacaacgaaa acggcaccat caccgacgcc
     6481 gtggactgcg ctctggaccc actgtctgag accaagtgca ccctgaagtc tttcaccgtg
     6541 gaaaagggaa tctaccagac cagcaacttc cgcgtgcagc ccaccgagtc tatcgtgaga
     6601 ttcccaaaca tcaccaacct gtgcccattc ggagaagtgt tcaacgctac caggttcgcc
     6661 agcgtgtacg cttggaacag gaagagaatc agcaactgcg tggccgacta ctctgtgctg
     6721 tacaactctg cctccttctc caccttcaag tgctacggcg tgtccccaac caagctgaac
     6781 gacctgtgct tcaccaacgt gtacgctgac tccttcgtga tcagaggaga cgaagtgaga
     6841 cagatcgctc ccggtcagac cggaaagatc gccgactaca actacaagct gccagacgac
     6901 ttcaccggtt gcgtgatcgc ttggaactct aacaacctgg actctaaggt gggaggcaac
     6961 tacaactacc tgtacaggct gttccgcaag agcaacctga agcctttcga aagggacatc
     7021 agcaccgaaa tctaccaggc tggatctacc ccatgcaacg gagtggaagg attcaactgc
     7081 tacttccctc tgcagtctta cggattccag ccaaccaacg gagtgggata ccagccatac
     7141 agagtggtgg tgctgtcttt cgagctgctg cacgctcctg ctaccgtgtg cggaccaaag
     7201 aagtctacca acctggtgaa gaacaagtgc gtgaacttca acttcaacgg actgaccgga
     7261 accggagtgc tgaccgaatc caacaagaag ttcctgcctt tccagcagtt cggtagagac
     7321 atcgccgaca ccaccgacgc tgtgagagac ccacagaccc tggaaatcct ggacatcacc
     7381 ccctgctctt tcggaggagt gagcgtgatc accccaggaa ccaacacctc caaccaggtg
     7441 gccgtgctgt accaggacgt gaactgcacc gaagtgccag tggccatcca cgctgaccag
     7501 ctgaccccaa cctggagagt gtacagcacc ggatctaacg tgttccagac ccgtgccggc
     7561 tgcctgatcg gagccgagca cgtgaacaac tcctacgaat gcgacatccc aatcggagct
     7621 ggcatctgcg cttcttacca gacccagacc aacagcccaa gaagggccag atccgtggcc
     7681 tctcagagca tcatcgccta caccatgagc ctgggcgctg aaaacagcgt ggcttacagc
     7741 aacaactcta tcgccatccc aaccaacttc accatctctg tgaccaccga aatcctgcca
     7801 gtgagcatga ccaagaccag cgtggactgc accatgtaca tctgcggaga ctctaccgaa
     7861 tgctctaacc tgctgctgca gtacggcagc ttctgcaccc agctgaaccg cgctctgacc
     7921 ggaatcgctg tggagcagga caagaacacc caggaagtgt tcgctcaggt gaagcagata
     7981 tacaagaccc cacctatcaa ggacttcgga ggattcaact tctctcagat cctgcccgac
     8041 ccatctaagc cctctaagag gtctttcatc gaagacctgc tgttcaacaa ggtgaccctg
     8101 gccgacgccg gtttcatcaa gcagtacggt gactgcctgg gagacatcgc cgctagagac
     8161 ctgatctgcg cccagaagtt caacggtctg accgtgctgc caccactgct gaccgacgag
     8221 atgatcgccc agtacacctc tgccctgctg gctggaacca tcacctctgg ttggaccttc
     8281 ggagctggcg ccgccctgca gatcccattc gctatgcaga tggcctaccg tttcaacggt
     8341 atcggagtga cccagaacgt gctgtacgaa aaccagaagc tgatcgctaa ccagttcaac
     8401 tctgctatcg gaaagatcca ggactccctg tcctctaccg cttctgccct gggaaagctg
     8461 caggacgtgg tgaaccagaa cgcccaggcc ctgaacaccc tggtgaagca gctgtcttct
     8521 aacttcggag ccatctcctc tgtgctgaac gacatcctgt ctaggctgga caaggtggaa
     8581 gccgaggtgc agatcgacag actgatcacc ggaagactgc agtctctgca gacctacgtg
     8641 acccagcagc tgatcagggc cgctgaaatc agagcttctg ccaacctggc tgccaccaag
     8701 atgtccgaat gcgtgctggg ccagtctaag agggtggact tctgcggaaa gggataccac
     8761 ctgatgtcct tcccacagtc tgctccacac ggagtggtgt tcctgcacgt gacctacgtg
     8821 cccgcccagg aaaagaactt caccaccgcc ccagctatct gccacgacgg aaaggctcac
     8881 ttccccaggg agggagtgtt cgtgtctaac ggaacccact ggttcgtgac ccagaggaac
     8941 ttctacgagc cacagatcat caccaccgac aacaccttcg tgtctggaaa ctgcgacgtg
     9001 gtgatcggaa tcgtgaacaa caccgtgtac gacccactgc agccagaact ggactctttc
     9061 aaggaggaac tggacaagta cttcaagaac cacacctctc cagacgtgga cctgggagac
     9121 atctccggaa tcaacgcctc tgtggtgaac atccagaagg aaatcgacag actgaacgag
     9181 gtggccaaga acctgaacga atctctgatc gacctgcagg aactgggaaa gtacgagcag
     9241 tacatcaagt ggccctggta catctggctg ggtttcatcg ctggtctgat cgctatcgtg
     9301 atggtgacca tcatgctgtg ctgcatgacc tcttgctgct cttgcctgaa gggatgctgc
     9361 tcttgcggat cttgctgcaa gttcgacgag gacgactccg agcctgtgct gaagggtgtg
     9421 aagctgcact acacctaccc aactttcttg tacaaagtgg ttgatatcaa ggccgccgac
     9481 atcaccagcc tgtacaagaa agtggggtgg agccatcccc agttcgagaa gggcggagga
     9541 tctggcggcg gaagcggcgg aggaagctgg tcccaccctc agtttgagaa aggcaccgag
     9601 ctgggcagca ccatggccag ctacccctac gacgtgcccg actacgcctg atgactcgag
     9661 tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     9721 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     9781 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     9841 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     9901 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     9961 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
    10021 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
    10081 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
    10141 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
    10201 astgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
    10261 aatarkssws wmttswycsm
//
