LOCUS       sequence-386198-       10143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 S_E484K in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 S_E484K
FEATURES             Location/Qualifiers
     source          1..10143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(110..127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(281..869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(361..380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1040..1831,1832..1900))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1663..1682
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1901..2005)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2080..2099)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2271..2650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2651..2854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2805..2825
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2877..2895
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2905..2929
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3072..3096
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6916..6940)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6987..7010
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7047..7070
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7101..7127
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             7101..7127
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(7128..7475,7476..7478,7479..8087)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(8131..8148)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8137..8361
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8494..8513)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8645..8692
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8700..9722
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8700..9722
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9852..9973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9889..9908)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9943..9962
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10022..10038)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10022..10038)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10035..10057)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10046..10062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10070..10076,10077..10094,10095..10100))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10115..10136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
       61 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      721 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1021 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1081 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1141 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1201 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1261 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1321 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1381 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1441 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1501 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1561 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1621 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1681 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1741 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1801 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1861 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1921 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1981 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     2041 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     2101 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     2161 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     2221 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
     2281 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     2341 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2401 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2461 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2521 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2581 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2641 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2701 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2761 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2821 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
     2881 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
     2941 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     3001 agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc
     3061 tgcagatatc aacaagtttg tacaaaaaag ttggcatgtt cgtgttcctg gtgctgctgc
     3121 cactggtgag ctcccagtgc gtgaacctga ccaccagaac ccagctgcca cctgcctaca
     3181 ccaactcctt caccaggggc gtgtactacc cagacaaggt gttcagatcc agcgtgctgc
     3241 actctaccca ggacctgttc ctgccattct tctctaacgt gacctggttc cacgccatcc
     3301 acgtgtctgg aaccaacggc accaagagat tcgacaaccc agtgctgcca ttcaacgacg
     3361 gcgtgtactt cgcttctacc gagaagtcta acatcatcag gggttggatc ttcggaacca
     3421 ccctggactc taagacccag tctctgctga tcgtgaacaa cgctaccaac gtggtgatca
     3481 aggtgtgcga gttccagttc tgcaacgacc cattcctggg agtgtactac cacaagaaca
     3541 acaagtcttg gatggaaagc gagttcaggg tgtacagctc tgctaacaac tgcaccttcg
     3601 agtacgtgtc tcagcctttc ctgatggacc tggaaggaaa gcagggaaac ttcaagaacc
     3661 tgagagagtt cgtgttcaag aacatcgacg gttacttcaa gatatacagc aagcacaccc
     3721 caatcaacct ggtgagggac ctgccacagg gtttctctgc tctggagccc ctggtggacc
     3781 tgccaatcgg catcaacatc accaggttcc agaccctgct ggctctgcac aggtcttacc
     3841 tgaccccagg cgacagcagc tctggatgga ccgccggcgc cgctgcttac tacgtgggat
     3901 acctgcagcc aaggaccttc ctgctgaagt acaacgaaaa cggcaccatc accgacgccg
     3961 tggactgcgc tctggaccca ctgtctgaga ccaagtgcac cctgaagtct ttcaccgtgg
     4021 aaaagggaat ctaccagacc agcaacttcc gcgtgcagcc caccgagtct atcgtgagat
     4081 tcccaaacat caccaacctg tgcccattcg gagaagtgtt caacgctacc aggttcgcca
     4141 gcgtgtacgc ttggaacagg aagagaatca gcaactgcgt ggccgactac tctgtgctgt
     4201 acaactctgc ctccttctcc accttcaagt gctacggcgt gtccccaacc aagctgaacg
     4261 acctgtgctt caccaacgtg tacgctgact ccttcgtgat cagaggagac gaagtgagac
     4321 agatcgctcc cggtcagacc ggaaagatcg ccgactacaa ctacaagctg ccagacgact
     4381 tcaccggttg cgtgatcgct tggaactcta acaacctgga ctctaaggtg ggaggcaact
     4441 acaactacct gtacaggctg ttccgcaaga gcaacctgaa gcctttcgaa agggacatca
     4501 gcaccgaaat ctaccaggct ggatctaccc catgcaacgg agtgaaagga ttcaactgct
     4561 acttccctct gcagtcttac ggattccagc caaccaacgg agtgggatac cagccataca
     4621 gagtggtggt gctgtctttc gagctgctgc acgctcctgc taccgtgtgc ggaccaaaga
     4681 agtctaccaa cctggtgaag aacaagtgcg tgaacttcaa cttcaacgga ctgaccggaa
     4741 ccggagtgct gaccgaatcc aacaagaagt tcctgccttt ccagcagttc ggtagagaca
     4801 tcgccgacac caccgacgct gtgagagacc cacagaccct ggaaatcctg gacatcaccc
     4861 cctgctcttt cggaggagtg agcgtgatca ccccaggaac caacacctcc aaccaggtgg
     4921 ccgtgctgta ccaggacgtg aactgcaccg aagtgccagt ggccatccac gctgaccagc
     4981 tgaccccaac ctggagagtg tacagcaccg gatctaacgt gttccagacc cgtgccggct
     5041 gcctgatcgg agccgagcac gtgaacaact cctacgaatg cgacatccca atcggagctg
     5101 gcatctgcgc ttcttaccag acccagacca acagcccaag aagggccaga tccgtggcct
     5161 ctcagagcat catcgcctac accatgagcc tgggcgctga aaacagcgtg gcttacagca
     5221 acaactctat cgccatccca accaacttca ccatctctgt gaccaccgaa atcctgccag
     5281 tgagcatgac caagaccagc gtggactgca ccatgtacat ctgcggagac tctaccgaat
     5341 gctctaacct gctgctgcag tacggcagct tctgcaccca gctgaaccgc gctctgaccg
     5401 gaatcgctgt ggagcaggac aagaacaccc aggaagtgtt cgctcaggtg aagcagatat
     5461 acaagacccc acctatcaag gacttcggag gattcaactt ctctcagatc ctgcccgacc
     5521 catctaagcc ctctaagagg tctttcatcg aagacctgct gttcaacaag gtgaccctgg
     5581 ccgacgccgg tttcatcaag cagtacggtg actgcctggg agacatcgcc gctagagacc
     5641 tgatctgcgc ccagaagttc aacggtctga ccgtgctgcc accactgctg accgacgaga
     5701 tgatcgccca gtacacctct gccctgctgg ctggaaccat cacctctggt tggaccttcg
     5761 gagctggcgc cgccctgcag atcccattcg ctatgcagat ggcctaccgt ttcaacggta
     5821 tcggagtgac ccagaacgtg ctgtacgaaa accagaagct gatcgctaac cagttcaact
     5881 ctgctatcgg aaagatccag gactccctgt cctctaccgc ttctgccctg ggaaagctgc
     5941 aggacgtggt gaaccagaac gcccaggccc tgaacaccct ggtgaagcag ctgtcttcta
     6001 acttcggagc catctcctct gtgctgaacg acatcctgtc taggctggac aaggtggaag
     6061 ccgaggtgca gatcgacaga ctgatcaccg gaagactgca gtctctgcag acctacgtga
     6121 cccagcagct gatcagggcc gctgaaatca gagcttctgc caacctggct gccaccaaga
     6181 tgtccgaatg cgtgctgggc cagtctaaga gggtggactt ctgcggaaag ggataccacc
     6241 tgatgtcctt cccacagtct gctccacacg gagtggtgtt cctgcacgtg acctacgtgc
     6301 ccgcccagga aaagaacttc accaccgccc cagctatctg ccacgacgga aaggctcact
     6361 tccccaggga gggagtgttc gtgtctaacg gaacccactg gttcgtgacc cagaggaact
     6421 tctacgagcc acagatcatc accaccgaca acaccttcgt gtctggaaac tgcgacgtgg
     6481 tgatcggaat cgtgaacaac accgtgtacg acccactgca gccagaactg gactctttca
     6541 aggaggaact ggacaagtac ttcaagaacc acacctctcc agacgtggac ctgggagaca
     6601 tctccggaat caacgcctct gtggtgaaca tccagaagga aatcgacaga ctgaacgagg
     6661 tggccaagaa cctgaacgaa tctctgatcg acctgcagga actgggaaag tacgagcagt
     6721 acatcaagtg gccctggtac atctggctgg gtttcatcgc tggtctgatc gctatcgtga
     6781 tggtgaccat catgctgtgc tgcatgacct cttgctgctc ttgcctgaag ggatgctgct
     6841 cttgcggatc ttgctgcaag ttcgacgagg acgactccga gcctgtgctg aagggtgtga
     6901 agctgcacta cacctaccca actttcttgt acaaagtggt tgatatcaag gccgccgaca
     6961 tcaccagcct gtacaagaaa gtggggtgga gccatcccca gttcgagaag ggcggaggat
     7021 ctggcggcgg aagcggcgga ggaagctggt cccaccctca gtttgagaaa ggcaccgagc
     7081 tgggcagcac catggccagc tacccctacg acgtgcccga ctacgccaag gacaacaccg
     7141 tgcccctgaa gctgatcgcc ctgctggcca acggcgagtt ccactctggc gagcagctgg
     7201 gcgagacact gggcatgagc agagccgcca tcaacaagca catccagacc ctgcgggact
     7261 ggggcgtgga cgtgttcaca gtgcccggca agggctacag cctgcccgag cccatccagc
     7321 tgctgaacgc caagcagatc ctgggccagc tggatggcgg atctgtggcc gtgctgcccg
     7381 tgatcgacag caccaaccag tatctgctcg accggatcgg cgagctgaag tccggcgacg
     7441 cctgtatcgc cgagtaccag caggccggca gaggcggcag gggcagaaag tggttcagcc
     7501 ccttcggcgc caacctgtac ctgagcatgt tctggagact ggaacagggc ccagccgccg
     7561 ctattggcct gagcctggtc atcggcatcg tgatggccga ggtgctgcgg aagctgggcg
     7621 ccgacaaagt gcgcgtgaag tggcccaacg acctgtatct gcaggaccgg aagctggccg
     7681 gcatcctggt ggaactgacc ggcaaaacag gcgacgccgc ccagatcgtg atcggcgctg
     7741 gcatcaacat ggccatgcgg cgggtggaag agagcgtggt caaccagggc tggatcaccc
     7801 tgcaggaagc cggcatcaac ctggaccgga acaccctggc cgccatgctg atcagagagc
     7861 tgagagccgc cctggaactg ttcgagcagg aaggcctggc cccctacctg agcagatggg
     7921 agaagctgga caacttcatc aaccggcccg tgaaactgat catcggcgac aaagagatct
     7981 tcggcatcag ccggggcatc gacaagcagg gggccctgct cctggaacag gacggcatca
     8041 tcaagccctg gatgggcggc gagatcagcc tgcggagcgc cgagaagtga tgactcgagt
     8101 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     8161 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     8221 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     8281 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     8341 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     8401 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     8461 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     8521 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     8581 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     8641 cctagaagtt cctattccga agttcctatt ctctagaaag tataggaact tccttggcca
     8701 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     8761 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     8821 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     8881 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     8941 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     9001 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     9061 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     9121 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     9181 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     9241 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     9301 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     9361 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     9421 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     9481 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     9541 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     9601 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     9661 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     9721 agcacgtact acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     9781 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     9841 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     9901 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     9961 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
    10021 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    10081 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    10141 cgt
//
