LOCUS       sequence-386201-        7739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 S_K417N in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 7739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 S_K417N
FEATURES             Location/Qualifiers
     source          1..7739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(571..595)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4417..4441)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4584..4608)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4618..4636
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4639..4657
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(4659..4862)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4688..4708)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4863..5242
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5414..5433
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5508..5612
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5613..5681,5682..6473)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5831..5850)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6644..7232
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7133..7152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7386..7403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7651..7732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7688..7707)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctctcaggtt cttgaagttt ccctgctttc cttccaggtc catcaggaaa ggctgagaca
       61 cgtactcgaa ggtgcagttg ttagcagagc tgtacaccct gaactcgctt tccatccaag
      121 acttgttgtt cttgtggtag tacactccca ggaatgggtc gttgcagaac tggaactcgc
      181 acaccttgat caccacgttg gtagcgttgt tcacgatcag cagagactgg gtcttagagt
      241 ccagggtggt tccgaagatc caacccctga tgatgttaga cttctcggta gaagcgaagt
      301 acacgccgtc gttgaatggc agcactgggt tgtcgaatct cttggtgccg ttggttccag
      361 acacgtggat ggcgtggaac caggtcacgt tagagaagaa tggcaggaac aggtcctggg
      421 tagagtgcag cacgctggat ctgaacacct tgtctgggta gtacacgccc ctggtgaagg
      481 agttggtgta ggcaggtggc agctgggttc tggtggtcag gttcacgcac tgggagctca
      541 ccagtggcag cagcaccagg aacacgaaca tgccaacttt tttgtacaaa gttgggtagg
      601 tgtagtgcag cttcacaccc ttcagcacag gctcggagtc gtcctcgtcg aacttgcagc
      661 aagatccgca agagcagcat cccttcaggc aagagcagca agaggtcatg cagcacagca
      721 tgatggtcac catcacgata gcgatcagac cagcgatgaa acccagccag atgtaccagg
      781 gccacttgat gtactgctcg tactttccca gttcctgcag gtcgatcaga gattcgttca
      841 ggttcttggc cacctcgttc agtctgtcga tttccttctg gatgttcacc acagaggcgt
      901 tgattccgga gatgtctccc aggtccacgt ctggagaggt gtggttcttg aagtacttgt
      961 ccagttcctc cttgaaagag tccagttctg gctgcagtgg gtcgtacacg gtgttgttca
     1021 cgattccgat caccacgtcg cagtttccag acacgaaggt gttgtcggtg gtgatgatct
     1081 gtggctcgta gaagttcctc tgggtcacga accagtgggt tccgttagac acgaacactc
     1141 cctccctggg gaagtgagcc tttccgtcgt ggcagatagc tggggcggtg gtgaagttct
     1201 tttcctgggc gggcacgtag gtcacgtgca ggaacaccac tccgtgtgga gcagactgtg
     1261 ggaaggacat caggtggtat ccctttccgc agaagtccac cctcttagac tggcccagca
     1321 cgcattcgga catcttggtg gcagccaggt tggcagaagc tctgatttca gcggccctga
     1381 tcagctgctg ggtcacgtag gtctgcagag actgcagtct tccggtgatc agtctgtcga
     1441 tctgcacctc ggcttccacc ttgtccagcc tagacaggat gtcgttcagc acagaggaga
     1501 tggctccgaa gttagaagac agctgcttca ccagggtgtt cagggcctgg gcgttctggt
     1561 tcaccacgtc ctgcagcttt cccagggcag aagcggtaga ggacagggag tcctggatct
     1621 ttccgatagc agagttgaac tggttagcga tcagcttctg gttttcgtac agcacgttct
     1681 gggtcactcc gataccgttg aaacggtagg ccatctgcat agcgaatggg atctgcaggg
     1741 cggcgccagc tccgaaggtc caaccagagg tgatggttcc agccagcagg gcagaggtgt
     1801 actgggcgat catctcgtcg gtcagcagtg gtggcagcac ggtcagaccg ttgaacttct
     1861 gggcgcagat caggtctcta gcggcgatgt ctcccaggca gtcaccgtac tgcttgatga
     1921 aaccggcgtc ggccagggtc accttgttga acagcaggtc ttcgatgaaa gacctcttag
     1981 agggcttaga tgggtcgggc aggatctgag agaagttgaa tcctccgaag tccttgatag
     2041 gtggggtctt gtatatctgc ttcacctgag cgaacacttc ctgggtgttc ttgtcctgct
     2101 ccacagcgat tccggtcaga gcgcggttca gctgggtgca gaagctgccg tactgcagca
     2161 gcaggttaga gcattcggta gagtctccgc agatgtacat ggtgcagtcc acgctggtct
     2221 tggtcatgct cactggcagg atttcggtgg tcacagagat ggtgaagttg gttgggatgg
     2281 cgatagagtt gttgctgtaa gccacgctgt tttcagcgcc caggctcatg gtgtaggcga
     2341 tgatgctctg agaggccacg gatctggccc ttcttgggct gttggtctgg gtctggtaag
     2401 aagcgcagat gccagctccg attgggatgt cgcattcgta ggagttgttc acgtgctcgg
     2461 ctccgatcag gcagccggca cgggtctgga acacgttaga tccggtgctg tacactctcc
     2521 aggttggggt cagctggtca gcgtggatgg ccactggcac ttcggtgcag ttcacgtcct
     2581 ggtacagcac ggccacctgg ttggaggtgt tggttcctgg ggtgatcacg ctcactcctc
     2641 cgaaagagca gggggtgatg tccaggattt ccagggtctg tgggtctctc acagcgtcgg
     2701 tggtgtcggc gatgtctcta ccgaactgct ggaaaggcag gaacttcttg ttggattcgg
     2761 tcagcactcc ggttccggtc agtccgttga agttgaagtt cacgcacttg ttcttcacca
     2821 ggttggtaga cttctttggt ccgcacacgg tagcaggagc gtgcagcagc tcgaaagaca
     2881 gcaccaccac tctgtatggc tggtatccca ctccgttggt tggctggaat ccgtaagact
     2941 gcagagggaa gtagcagttg aatccttcca ctccgttgca tggggtagat ccagcctggt
     3001 agatttcggt gctgatgtcc ctttcgaaag gcttcaggtt gctcttgcgg aacagcctgt
     3061 acaggtagtt gtagttgcct cccaccttag agtccaggtt gttagagttc caagcgatca
     3121 cgcaaccggt gaagtcgtct ggcagcttgt agttgtagtc ggcgatattt ccggtctgac
     3181 cgggagcgat ctgtctcact tcgtctcctc tgatcacgaa ggagtcagcg tacacgttgg
     3241 tgaagcacag gtcgttcagc ttggttgggg acacgccgta gcacttgaag gtggagaagg
     3301 aggcagagtt gtacagcaca gagtagtcgg ccacgcagtt gctgattctc ttcctgttcc
     3361 aagcgtacac gctggcgaac ctggtagcgt tgaacacttc tccgaatggg cacaggttgg
     3421 tgatgtttgg gaatctcacg atagactcgg tgggctgcac gcggaagttg ctggtctggt
     3481 agattccctt ttccacggtg aaagacttca gggtgcactt ggtctcagac agtgggtcca
     3541 gagcgcagtc cacggcgtcg gtgatggtgc cgttttcgtt gtacttcagc aggaaggtcc
     3601 ttggctgcag gtatcccacg tagtaagcag cggcgccggc ggtccatcca gagctgctgt
     3661 cgcctggggt caggtaagac ctgtgcagag ccagcagggt ctggaacctg gtgatgttga
     3721 tgccgattgg caggtccacc aggggctcca gagcagagaa accctgtggc aggtccctca
     3781 ccaggttgat tggggtgtgc ttgctgtata tcttgaagta accgtcgatg ttcttgaaca
     3841 cgaactctct caggttcttg aagtttccct gctttccttc caggtccatc aggaaaggct
     3901 gagacacgta ctcgaaggtg cagttgttag cagagctgta caccctgaac tcgctttcca
     3961 tccaagactt gttgttcttg tggtagtaca ctcccaggaa tgggtcgttg cagaactgga
     4021 actcgcacac cttgatcacc acgttggtag cgttgttcac gatcagcaga gactgggtct
     4081 tagagtccag ggtggttccg aagatccaac ccctgatgat gttagacttc tcggtagaag
     4141 cgaagtacac gccgtcgttg aatggcagca ctgggttgtc gaatctcttg gtgccgttgg
     4201 ttccagacac gtggatggcg tggaaccagg tcacgttaga gaagaatggc aggaacaggt
     4261 cctgggtaga gtgcagcacg ctggatctga acaccttgtc tgggtagtac acgcccctgg
     4321 tgaaggagtt ggtgtaggca ggtggcagct gggttctggt ggtcaggttc acgcactggg
     4381 agctcaccag tggcagcagc accaggaaca cgaacatgcc aacttttttg tacaaacttg
     4441 ttgatatctg cagaattcca ccacactgga ctagtggatc cgagctcggt accaagctta
     4501 agtttaaacg ctagagtccg gaggctggat cggtcccggt gtcttctatg gaggtcaaaa
     4561 cagcgtggat ggcgtctcca ggcgatctga cggttcacta aacgagctcg tcgacgatct
     4621 ctatcactga tagggagatc tctatcactg atagggagag ctctgcttat atagacctcc
     4681 caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga
     4741 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt
     4801 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc
     4861 accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag
     4921 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
     4981 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
     5041 acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt
     5101 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat
     5161 gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga ttactattaa
     5221 taactagtca ataatcaatg tcaacgcgta tatctggccc gtacatcgcg aagcagcgca
     5281 aaacgcctaa ccctaagcag attcttcatg caattgtcgg tcaagccttg ccttgttgta
     5341 gcttaaattt tgctcgcgca ctactcagcg acctccaaca cacaagcagg gagcagatac
     5401 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatag gggatcggga
     5461 gatctcccga tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5521 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5581 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5641 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5701 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5761 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5821 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5881 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5941 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     6001 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6061 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6121 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6181 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6241 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6301 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6361 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6421 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6481 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6541 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6601 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6661 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6721 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6781 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6841 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6901 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6961 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7021 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7081 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7141 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7201 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7261 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7321 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7381 agcgcagcga gtcagtgagc gaggaagcgg aagagcgctg ccggcacctg tcctacgagt
     7441 tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
     7501 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacggatcct tatcgatttt
     7561 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     7621 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     7681 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctag
//
