LOCUS       sequence-386202-        5096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses left-side TALE–DddAtox N term half cox3-FusXTBE in 
            zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Sabharwal A, Kar B, Restrepo-Castillo S, Holmberg SR, Mathew ND, 
            Kendall BL, Cotter RP, WareJoncas Z, Seiler C, Nakamaru-Ogiso E, 
            Clark KJ, Ekker SC
  TITLE     The FusX TALE Base Editor (FusXTBE) for Rapid Mitochondrial DNA 
            Programming of Human Cells In Vitro and Zebrafish Disease Models In 
            Vivo.
  JOURNAL   CRISPR J. 2021 Dec;4(6):799-821. doi: 10.1089/crispr.2021.0061. Epub
            2021 Nov 30.
  PUBMED    34847747
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2021.0061"; journalName: "CRISPR J"; date: 
            "2021-12"; volume: "4"; issue: "6"; pages: "799-821"
COMMENT     Sequence Label: Cox3-FusXTBE-Left TALE
FEATURES             Location/Qualifiers
     source          1..5096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..21
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     5'UTR           30..72
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             2760..3011
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     3'UTR           3059..3183
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     primer_bind     complement(3333..3352)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3344..4153
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3411..3430)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4296..4884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4785..4804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5038..5055
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaattaacc ctcactaaag ggaagcttgc ttgttctttt tgcagaagct cagaataaac
       61 gctcaacttt ggcagatctc accatggcag gttatttgaa ggtcctaagc tctctcagca
      121 ggtctgctgc cactctatcc aaaagccccg ccgttcttgc tccagcttgc cggagtttgc
      181 aacagaggaa ctatgccgat aaacgtatcc agtctaggga cgtcactaga gtggatctac
      241 gcacgctcgg ctacagtcag cagcagcaag agaagatcaa accgaaggtg cgttcgacag
      301 tggcgcagca ccacgaggca ctggtgggcc atgggtttac acacgcgcac atcgttgcgc
      361 tcagccaaca cccggcagcg ttagggaccg tcgctgtcac gtatcagcac ataatcacgg
      421 cgttgccaga ggcgacacac gaagacatcg ttggcgtcgg caaacagtgg tccggcgcac
      481 gcgccctgga ggccttgctc acggatgcgg gggagttgag aggtccgccg ttacagttgg
      541 acacaggcca acttgtgaag attgcaaaac gtggcggcgt gaccgcaatg gaggcagtgc
      601 atgcatcgcg caatgcactg acgggtgccc ccctgaacct gaccccggac caagtggtgg
      661 ctatcgccag caacaatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
      721 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacaatg
      781 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
      841 gcctgacccc ggaccaagtg gtggctatcg ccagcaacat tggcggcaag caagcgctcg
      901 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag
      961 tggtggctat cgccagcaac attggcggca agcaagcgct cgaaacggtg cagcggctgt
     1021 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca
     1081 acaatggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg
     1141 accatggcct gaccccggac caagtggtgg ctatcgccag caacaatggc ggcaagcaag
     1201 cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg
     1261 accaagtggt ggctatcgcc agcaacattg gcggcaagca agcgctcgaa acggtgcagc
     1321 ggctgttgcc ggtgctgtgc caggaccatg gcctcacacc cgatcaggtc gtggctatcg
     1381 ccagcaacaa tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
     1441 gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac attggcggca
     1501 agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga
     1561 ccccggacca agtggtggct atcgccagca acattggcgg caagcaagcg ctcgaaacgg
     1621 tgcagcggct gttgccggtg ctgtgccagg accatggcct gactccggac caagtggtgg
     1681 ctatcgccag ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
     1741 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacaatg
     1801 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
     1861 gcctgacccc ggaccaagtg gtggctatcg ccagcaacat tggcggcaag caagcgctcg
     1921 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag
     1981 tggtggctat cgccagcaac attggcggca agcaagcgct cgaaacggtg cagcggctgt
     2041 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca
     2101 acattggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg
     2161 accatggcct gaccccggac caagtggtgg ctatcgccag caacattggc ggcaagcaag
     2221 cgctcgaaag cattgtggcc cagctgagcc ggcctgatcc ggcgttggcc gcgttgacca
     2281 acgaccacct cgtcgccttg gcctgcctcg gcggacgtcc tgccatggat gcagtgaaaa
     2341 agggattgcc gcacgcgccg gaattgatca gaagagtcaa tcgccgtatt ggcgaacgca
     2401 cgtcccatcg cgttgcctct agaggcagct acgccctggg tccgtatcag attagcgccc
     2461 cgcagctgcc agcttacaat ggtcagaccg tgggtacctt ctactatgtg aacgacgcgg
     2521 gcggtctgga gagcaaggtg tttagcagcg gcggtccaac cccgtaccca aactatgcca
     2581 atgccggtca tgtggagggt cagagcgccc tgttcatgcg tgataacggc atcagcgagg
     2641 gtctggtgtt ccacaacaac ccggaaggca cctgcggttt ttgcgtgaac atgaccgaga
     2701 ccctgctgcc ggaaaacgcg aaaatgaccg tggtgccgcc ggaaggttct ggcggctcaa
     2761 ctaatctgag cgacatcatt gagaaggaga ctgggaaaca gctggtcatt caggagtcca
     2821 tcctgatgct gcctgaggag gtggaggaag tgatcggcaa caagccagag tctgacatcc
     2881 tggtgcacac cgcctacgac gagtccacag atgagaatgt gatgctgctg acctctgacg
     2941 cccccgagta taagccttgg gccctggtca tccaggattc taacggcgag aataagatca
     3001 agatgctgtg ataggcacta gttagtgact gactaggatc tggctagcgt taccactaaa
     3061 ccagcctcaa gaacacccga atggagtctc taagctacat aataccaact tacacttaca
     3121 aaatgttgtc ccccaaaatg tagccattcg tatctgctcc taataaaaag aaagtttctt
     3181 cacattctaa aaaraaaaar aaaaaaaaaa aaraarrwss rccwmcyyca ccymyccysc
     3241 ccgcatgcct gcaggtcgac taggatcact agtgcggccg cccgcggctg catcatgaac
     3301 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     3361 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     3421 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     3481 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     3541 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     3601 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     3661 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     3721 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     3781 cctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     3841 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     3901 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     3961 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     4021 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     4081 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     4141 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcaaaaag gatctaggtg
     4201 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4261 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4321 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4381 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4441 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4501 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4561 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4621 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4681 cgtgagctat gagaaagcgc cacggttccc gaagggagaa aggcggacag gtatccggta
     4741 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4801 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4861 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4921 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4981 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     5041 gagtcagtga gcgaggaagc ggaacagatc gacgatagag cgcccaatac gcaaac
//
