LOCUS       sequence-386207-       10134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 S_L241-243del in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10134)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 10134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 S_L241-243del
FEATURES             Location/Qualifiers
     source          1..10134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(99..116)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    105..329
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(462..481)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    613..660
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             668..1690
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             668..1690
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    1820..1941
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1857..1876)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1911..1930
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1990..2006)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1990..2006)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2003..2025)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2014..2030
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2038..2044,2045..2062,2063..2068))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2083..2104
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2221..2238)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2392..2980)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2472..2491)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3151..3942,3943..4011))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3774..3793
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4012..4116)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4191..4210)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4382..4761
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4762..4965
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4916..4936
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4967..4985
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4988..5006
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     5016..5040
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    5183..5207
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9018..9042)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9089..9112
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             9149..9172
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             9203..9229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             9203..9229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(9230..9577,9578..9580,9581..10134,1..55)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
ORIGIN
        1 cggcatcatc aagccctgga tgggcggcga gatcagcctg cggagcgccg agaagtgatg
       61 actcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
      121 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
      181 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
      241 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
      301 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
      361 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      421 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      481 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
      541 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
      601 ttcacgtacc tagaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
      661 cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
      721 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
      781 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
      841 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
      901 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
      961 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     1021 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     1081 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     1141 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     1201 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     1261 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     1321 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     1381 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     1441 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     1501 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     1561 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     1621 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     1681 aaaggaatag cacgtactac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     1741 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     1801 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     1861 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1921 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     1981 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     2041 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     2101 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     2161 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     2221 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2281 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2341 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2401 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2461 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2521 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2581 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2641 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2701 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2761 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2821 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2881 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2941 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3001 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3061 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3121 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3181 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3241 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3301 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3361 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3421 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3481 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3541 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3601 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3661 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3721 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3781 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3841 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3901 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3961 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     4021 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     4081 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     4141 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     4201 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     4261 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     4321 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     4381 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     4441 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     4501 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     4561 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     4621 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     4681 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     4741 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     4801 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     4861 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     4921 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
     4981 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
     5041 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
     5101 ctccggactc tagcgtttaa acttaagctt ggtaccgagc tcggatccac tagtccagtg
     5161 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gttggcatgt tcgtgttcct
     5221 ggtgctgctg ccactggtga gctcccagtg cgtgaacctg accaccagaa cccagctgcc
     5281 acctgcctac accaactcct tcaccagggg cgtgtactac ccagacaagg tgttcagatc
     5341 cagcgtgctg cactctaccc aggacctgtt cctgccattc ttctctaacg tgacctggtt
     5401 ccacgccatc cacgtgtctg gaaccaacgg caccaagaga ttcgacaacc cagtgctgcc
     5461 attcaacgac ggcgtgtact tcgcttctac cgagaagtct aacatcatca ggggttggat
     5521 cttcggaacc accctggact ctaagaccca gtctctgctg atcgtgaaca acgctaccaa
     5581 cgtggtgatc aaggtgtgcg agttccagtt ctgcaacgac ccattcctgg gagtgtacta
     5641 ccacaagaac aacaagtctt ggatggaaag cgagttcagg gtgtacagct ctgctaacaa
     5701 ctgcaccttc gagtacgtgt ctcagccttt cctgatggac ctggaaggaa agcagggaaa
     5761 cttcaagaac ctgagagagt tcgtgttcaa gaacatcgac ggttacttca agatatacag
     5821 caagcacacc ccaatcaacc tggtgaggga cctgccacag ggtttctctg ctctggagcc
     5881 cctggtggac ctgccaatcg gcatcaacat caccaggttc cagaccctgc acaggtctta
     5941 cctgacccca ggcgacagca gctctggatg gaccgccggc gccgctgctt actacgtggg
     6001 atacctgcag ccaaggacct tcctgctgaa gtacaacgaa aacggcacca tcaccgacgc
     6061 cgtggactgc gctctggacc cactgtctga gaccaagtgc accctgaagt ctttcaccgt
     6121 ggaaaaggga atctaccaga ccagcaactt ccgcgtgcag cccaccgagt ctatcgtgag
     6181 attcccaaac atcaccaacc tgtgcccatt cggagaagtg ttcaacgcta ccaggttcgc
     6241 cagcgtgtac gcttggaaca ggaagagaat cagcaactgc gtggccgact actctgtgct
     6301 gtacaactct gcctccttct ccaccttcaa gtgctacggc gtgtccccaa ccaagctgaa
     6361 cgacctgtgc ttcaccaacg tgtacgctga ctccttcgtg atcagaggag acgaagtgag
     6421 acagatcgct cccggtcaga ccggaaagat cgccgactac aactacaagc tgccagacga
     6481 cttcaccggt tgcgtgatcg cttggaactc taacaacctg gactctaagg tgggaggcaa
     6541 ctacaactac ctgtacaggc tgttccgcaa gagcaacctg aagcctttcg aaagggacat
     6601 cagcaccgaa atctaccagg ctggatctac cccatgcaac ggagtggaag gattcaactg
     6661 ctacttccct ctgcagtctt acggattcca gccaaccaac ggagtgggat accagccata
     6721 cagagtggtg gtgctgtctt tcgagctgct gcacgctcct gctaccgtgt gcggaccaaa
     6781 gaagtctacc aacctggtga agaacaagtg cgtgaacttc aacttcaacg gactgaccgg
     6841 aaccggagtg ctgaccgaat ccaacaagaa gttcctgcct ttccagcagt tcggtagaga
     6901 catcgccgac accaccgacg ctgtgagaga cccacagacc ctggaaatcc tggacatcac
     6961 cccctgctct ttcggaggag tgagcgtgat caccccagga accaacacct ccaaccaggt
     7021 ggccgtgctg taccaggacg tgaactgcac cgaagtgcca gtggccatcc acgctgacca
     7081 gctgacccca acctggagag tgtacagcac cggatctaac gtgttccaga cccgtgccgg
     7141 ctgcctgatc ggagccgagc acgtgaacaa ctcctacgaa tgcgacatcc caatcggagc
     7201 tggcatctgc gcttcttacc agacccagac caacagccca agaagggcca gatccgtggc
     7261 ctctcagagc atcatcgcct acaccatgag cctgggcgct gaaaacagcg tggcttacag
     7321 caacaactct atcgccatcc caaccaactt caccatctct gtgaccaccg aaatcctgcc
     7381 agtgagcatg accaagacca gcgtggactg caccatgtac atctgcggag actctaccga
     7441 atgctctaac ctgctgctgc agtacggcag cttctgcacc cagctgaacc gcgctctgac
     7501 cggaatcgct gtggagcagg acaagaacac ccaggaagtg ttcgctcagg tgaagcagat
     7561 atacaagacc ccacctatca aggacttcgg aggattcaac ttctctcaga tcctgcccga
     7621 cccatctaag ccctctaaga ggtctttcat cgaagacctg ctgttcaaca aggtgaccct
     7681 ggccgacgcc ggtttcatca agcagtacgg tgactgcctg ggagacatcg ccgctagaga
     7741 cctgatctgc gcccagaagt tcaacggtct gaccgtgctg ccaccactgc tgaccgacga
     7801 gatgatcgcc cagtacacct ctgccctgct ggctggaacc atcacctctg gttggacctt
     7861 cggagctggc gccgccctgc agatcccatt cgctatgcag atggcctacc gtttcaacgg
     7921 tatcggagtg acccagaacg tgctgtacga aaaccagaag ctgatcgcta accagttcaa
     7981 ctctgctatc ggaaagatcc aggactccct gtcctctacc gcttctgccc tgggaaagct
     8041 gcaggacgtg gtgaaccaga acgcccaggc cctgaacacc ctggtgaagc agctgtcttc
     8101 taacttcgga gccatctcct ctgtgctgaa cgacatcctg tctaggctgg acaaggtgga
     8161 agccgaggtg cagatcgaca gactgatcac cggaagactg cagtctctgc agacctacgt
     8221 gacccagcag ctgatcaggg ccgctgaaat cagagcttct gccaacctgg ctgccaccaa
     8281 gatgtccgaa tgcgtgctgg gccagtctaa gagggtggac ttctgcggaa agggatacca
     8341 cctgatgtcc ttcccacagt ctgctccaca cggagtggtg ttcctgcacg tgacctacgt
     8401 gcccgcccag gaaaagaact tcaccaccgc cccagctatc tgccacgacg gaaaggctca
     8461 cttccccagg gagggagtgt tcgtgtctaa cggaacccac tggttcgtga cccagaggaa
     8521 cttctacgag ccacagatca tcaccaccga caacaccttc gtgtctggaa actgcgacgt
     8581 ggtgatcgga atcgtgaaca acaccgtgta cgacccactg cagccagaac tggactcttt
     8641 caaggaggaa ctggacaagt acttcaagaa ccacacctct ccagacgtgg acctgggaga
     8701 catctccgga atcaacgcct ctgtggtgaa catccagaag gaaatcgaca gactgaacga
     8761 ggtggccaag aacctgaacg aatctctgat cgacctgcag gaactgggaa agtacgagca
     8821 gtacatcaag tggccctggt acatctggct gggtttcatc gctggtctga tcgctatcgt
     8881 gatggtgacc atcatgctgt gctgcatgac ctcttgctgc tcttgcctga agggatgctg
     8941 ctcttgcgga tcttgctgca agttcgacga ggacgactcc gagcctgtgc tgaagggtgt
     9001 gaagctgcac tacacctacc caactttctt gtacaaagtg gttgatatca aggccgccga
     9061 catcaccagc ctgtacaaga aagtggggtg gagccatccc cagttcgaga agggcggagg
     9121 atctggcggc ggaagcggcg gaggaagctg gtcccaccct cagtttgaga aaggcaccga
     9181 gctgggcagc accatggcca gctaccccta cgacgtgccc gactacgcca aggacaacac
     9241 cgtgcccctg aagctgatcg ccctgctggc caacggcgag ttccactctg gcgagcagct
     9301 gggcgagaca ctgggcatga gcagagccgc catcaacaag cacatccaga ccctgcggga
     9361 ctggggcgtg gacgtgttca cagtgcccgg caagggctac agcctgcccg agcccatcca
     9421 gctgctgaac gccaagcaga tcctgggcca gctggatggc ggatctgtgg ccgtgctgcc
     9481 cgtgatcgac agcaccaacc agtatctgct cgaccggatc ggcgagctga agtccggcga
     9541 cgcctgtatc gccgagtacc agcaggccgg cagaggcggc aggggcagaa agtggttcag
     9601 ccccttcggc gccaacctgt acctgagcat gttctggaga ctggaacagg gcccagccgc
     9661 cgctattggc ctgagcctgg tcatcggcat cgtgatggcc gaggtgctgc ggaagctggg
     9721 cgccgacaaa gtgcgcgtga agtggcccaa cgacctgtat ctgcaggacc ggaagctggc
     9781 cggcatcctg gtggaactga ccggcaaaac aggcgacgcc gcccagatcg tgatcggcgc
     9841 tggcatcaac atggccatgc ggcgggtgga agagagcgtg gtcaaccagg gctggatcac
     9901 cctgcaggaa gccggcatca acctggaccg gaacaccctg gccgccatgc tgatcagaga
     9961 gctgagagcc gccctggaac tgttcgagca ggaaggcctg gccccctacc tgagcagatg
    10021 ggagaagctg gacaacttca tcaaccggcc cgtgaaactg atcatcggcg acaaagagat
    10081 cttcggcatc agccggggca tcgacaagca gggggccctg ctcctggaac agga
//
