LOCUS       sequence-386212-        9566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for integrating tetO7-controlled sfGFP into the HO region in
            yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Takhaveev V, Ozsezen S, Smith EN, Zylstra A, Chaillet ML, Chen H, 
            Papagiannakis A, Milias-Argeitis A, Heinemann M
  TITLE     Temporal segregation of biosynthetic processes is responsible for 
            metabolic oscillations during the budding yeast cell cycle.
  JOURNAL   Nat Metab. 2023 Feb;5(2):294-313. doi: 10.1038/s42255-023-00741-x. 
            Epub 2023 Feb 27.
  PUBMED    36849832
REFERENCE   2  (bases 1 to 9566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-023-00741-x"; journalName: "Nat Metab"; date: 
            "2023-02"; volume: "5"; issue: "2"; pages: "294-313"
COMMENT     Sequence Label: pB_tetO7_sfGFP
FEATURES             Location/Qualifiers
     source          1..9566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1013..1399,1400..2020))
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     1928..1947
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(2105..2129)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(2126..2329)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2155..2175)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2330..2709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     terminator      2821..3008
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2876..2900)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    join(3066..3084,3085..3107,3108..3126,3127..3149,3150..3168,
                     3169..3191,3192..3210,3211..3233,3234..3252,3253..3275,
                     3276..3294,3295..3317,3318..3336)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     terminator      4254..4501
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4270..4288)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     gene            4573..5929
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4573..4916
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             4917..5726
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4984..5003)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      5732..5929
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     6499..6515
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6499..6515
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6529..6548)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6530..6548)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6555..6572)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6555..6571)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6564..6586)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6871..6890)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7003..7024
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7178..7197)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7297..7319
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7357..7375)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7443..7547
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7548..7616,7617..8408)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7766..7785)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8579..9167
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9068..9087
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9321..9338
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9455..9476
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    9531..9547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9536..9558
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(9555..9566,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(9555..9566,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccact agtccgaggc ctcgagatcc gatatcgccg tggcggccgc
       61 cagctgaagc ttaattatcc tgggcacgag tgaaacaaag ctaaaacctt tatttagcat
      121 ggccattgaa tgtaacaatt atatatatcg caagcacaaa aaatcaagga gagagaacta
      181 ccactttgtt catgtgtaca atgttcatta tctccataag caaaaaaaaa aaaatagaaa
      241 acatatgcta taaggttgat attctcacga gtaagcggca cttgctactt attgacattg
      301 cagatttttg gctacagaaa tagtatatta gagattataa ttgctaatca aatcaaaata
      361 taaaattagt aaaccaaacc atttataccc ttccttagta gttatggatt gttttttaat
      421 gatatttctg caaaccaaag aaagattgtt atccagatag aatttagttt tgatattcat
      481 ttttttgttg aagattgaac gccatatctg ggcctcataa ttcaaaagac ggtgccatta
      541 tcggtagcgt ttcgcattgt actggatttc agaaatttca cagttgatga atcgaaaaga
      601 atggtctcat tgcaacacgt aaggttaaga tgtccctttt taccattata ggcaataaat
      661 gaatcataaa acgaccgtat actggtgaaa tagtagggag aacgagtacc tgtagtaaaa
      721 agtataaatc atagttaatc gtgcaatgtc cctcgatcaa ggagtattgt gtcatgttcg
      781 agacaaacgc caacattttt gtttcttttg gacaaatgtt gtttgcattt atgatccgtt
      841 atattttgat ctaatgtaga gttgcacgta gttcttactg gcaaagaaat cgatgcatac
      901 caaaaaagaa taaaggtgat atttgatctt taccgtttag ttccaacgta aaattgtgcc
      961 tttggactta aaatggcgtc gtacggaatt cttattacga tcctcgcgcc ccctacccac
     1021 cgtactcgtc aattccaagg gcatcggtaa acatctgctc aaactcgaag tcggccatat
     1081 ccagagcgcc gtagggggcg gagtcgtggg gggtaaatcc cggacccggg gaatccccgt
     1141 cccccaacat gtccagatcg aaatcgtcta gcgcgtcggc atgcgccatc gccacgtcct
     1201 cgccgtctaa gtggagctcg tcccccaggc tgacatcggt cgggggggcc gtcgacagtc
     1261 tgcgcgtgtg tcccgcgggg agaaaggaca ggcgcggagc cgccagcccc gcctcttcgg
     1321 gggcgtcgtc gtccgggaga tcgagcaggc cctcgatggt agacccgtaa ttgtttttcg
     1381 tacgcgcgcg gctgtacgcg gacccacttt cacatttaag ttgtttttct aatccgcata
     1441 tgatcaattc aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga
     1501 tagcttgtcg taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag
     1561 cgacttgatg ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga
     1621 gtgcatataa tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt
     1681 cgagagtttc atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc
     1741 gatgacttag taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc
     1801 tttccccttc taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg
     1861 cgtcgagcaa agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta
     1921 gcttctgggc gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta
     1981 atgcgctgtt aatcacttta cttttatcta atctagacat atgaattaat tcggggccgc
     2041 ggaggctgga tcggtcccgg tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc
     2101 aggcgatctg acggttcact aaacgagctc tgcttatata gacctcccac cgtacacgcc
     2161 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat
     2221 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
     2281 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag
     2341 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     2401 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     2461 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     2521 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     2581 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     2641 ccatatatgg gctatgaact aatgaccctg taattgatta ctattaataa ctagtcaata
     2701 atcaatgtca acatggcggt aatgttggac atgagccaat ataaatgtac atattatgat
     2761 atggatacaa cgtatgcaat gggccaagct cctcgagtaa ttcgcgccac ttctaaataa
     2821 gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta
     2881 tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct
     2941 ttcctgtagg tcaggttgct ttctcaggta tagtatgagg tcgctcttat tgaccacacc
     3001 tctaccggca gatccgctag ggataacagg gtaatataga tcaattcctc gatcgagttt
     3061 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     3121 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     3181 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     3241 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     3301 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gctcggtacc
     3361 ctatggcatg catgtgctct gtatgtatat aaaactcttg ttttcttctt ttctctaaat
     3421 attctttcct tatacattag gtcctttgta gcataaatta ctatacttct atagacacgc
     3481 aaacacaaat acacacacta aattaccgga tcaattcggg atgtccaagg gtgaagagct
     3541 atttactggg gttgtaccca ttttggtaga actggacgga gatgtaaacg gacataaatt
     3601 ctctgttaga ggtgagggcg aaggcgatgc caccaatggt aaattgacta tgaagtttat
     3661 atgcactacg ggtaaattac ctgttccttg gccaacccta gtaacaactt tgacatatgg
     3721 tgttcaatgt ttctcaagat acccagacca tatgaaaagg catgatttct ttaaaagtgc
     3781 tatgccagaa ggctacgtgc aagagagaac tatctccttt aaggatgacg gtacgtataa
     3841 aacacgagca gaagtgaaat tcgaagggga tacactagtt aatcgcatcg aattaaaggg
     3901 tatagacttt aaggaagatg gtaatattct cggccataaa cttgagtata atttcaactc
     3961 gcataatgtg tacattacag ctgacaaaca aaagaacgga attaaagcga attttaaaat
     4021 caggcacaac gtcgaagatg ggtctgttca acttgccgat cattatcagc aaaacacccc
     4081 tattggtgat ggtccagtct tgttacccga taatcactac ttaagcacac agtctagatt
     4141 gtcaaaagat ccgaatgaaa agcgtgatca catggtttta ttggaatttg tcaccgctgc
     4201 aggaataact cacggaatgg acgagcttta taagggatcc taagagggcc gcatcatgta
     4261 attagttatg tcacgcttac attcacgccc tccccccaca tccgctctaa ccgaaaagga
     4321 aggagttaga caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt
     4381 aagaacgtta tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg
     4441 taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg ctttaatttg
     4501 cggccaagct tgttaattaa ggcgcgccag atctgtttac ttgcctcgtc cccgccgggt
     4561 cacccggcca gcgacatgga ggcccagaat accctccttg acagtcttga cgtgcgcagc
     4621 tcaggggcat gatgtgactg tcgcccgtac atttagccca tacatcccca tgtataatca
     4681 tttgcatcca tacattttga tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg
     4741 cagacctgcg agcagggaaa cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg
     4801 cccctgtaga gaaatataaa aggttaggat ttgccactga ggttcttctt tcatatactt
     4861 ccttttaaaa tcttgctagg atacagttct cacatcacat ccgaacataa acaaccatgg
     4921 gtaaggaaaa gactcacgtt tcgaggccgc gattaaattc caacatggat gctgatttat
     4981 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt
     5041 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     5101 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     5161 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggca
     5221 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     5281 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     5341 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     5401 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     5461 agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     5521 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     5581 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     5641 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     5701 atttgatgct cgatgagttt ttctaatcag tactgacaat aaaaagattc ttgttttcaa
     5761 gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag
     5821 cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca
     5881 gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg
     5941 atactaacgc cgccatccag tgtcgaaaac gagctcgaat tcctggggga acaacttcac
     6001 agaatgtttt gtcatattgt cgaagtggtc acaaaacaag agaagttccg ccaattataa
     6061 aaagggaacc cgtatatttc agcttcacgg atgatttcca gggtgagagt actgtatatg
     6121 ggcttacgat agaaggccat aaaaatttct tgcttggcaa caaaatagaa gtgaaatcat
     6181 gtcgaggctg ctgtgtggga gaacagcata aaatatcaca aaaaaagaat ctaaaacact
     6241 gtgttgcttg tcccagaaag ggaatcaagt atttttataa agattggagt ggtaaaaatc
     6301 gagtatgtgc tagatgctat ggaagataca aattcagcgg tcatcactgt ataaattgca
     6361 agtatgtacc agaagcacgt gaagtgaaaa aggcaaaaga caaaggcgaa aaattgggca
     6421 ttacgcccga aggtttgcca gttaaaggac cagagtgtat aaaatgtggc ggaatcttac
     6481 agtggcctat gcggccgctc tagaactagt ggatcgatcc ccaattcgcc ctatagtgag
     6541 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     6601 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6661 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     6721 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     6781 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6841 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6901 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg tgggccatcg ccctgataga
     6961 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7021 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
     7081 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     7141 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     7201 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     7261 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     7321 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     7381 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     7441 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     7501 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     7561 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7621 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7681 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7741 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7801 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7861 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7921 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7981 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8041 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8101 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8161 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8221 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8281 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     8341 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8401 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8461 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8521 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8581 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8641 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8701 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8761 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8821 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8881 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8941 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9001 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9061 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9121 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9181 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9241 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9301 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9361 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     9421 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     9481 caccccaggc tttacacttt atgcttccgc ggctcgtatg ttgtgtggaa ttgtgagcgg
     9541 ataacaattt cacacaggaa acagct
//
