LOCUS       sequence-386215-       11468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mNeon fluorescent protein fused to nucleocapsid protein in psPAX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11468)
  AUTHORS   Yu B, Shi Q, Belk JA, Yost KE, Parker KR, Li R, Liu BB, Huang H, 
            Lingwood D, Greenleaf WJ, Davis MM, Satpathy AT, Chang HY
  TITLE     Engineered cell entry links receptor biology with single-cell 
            genomics.
  JOURNAL   Cell. 2022 Dec 22;185(26):4904-4920.e22. doi: 
            10.1016/j.cell.2022.11.016. Epub 2022 Dec 13.
  PUBMED    36516854
REFERENCE   2  (bases 1 to 11468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11.016"; journalName: "Cell"; date: 
            "2022-12-22- 22"; volume: "185"; issue: "26"; pages: "4904-4920.e22"
COMMENT     Sequence Label: psPax2-D64V-NC-mNeon
FEATURES             Location/Qualifiers
     source          1..11468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3098..3802
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
                     LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
                     ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
                     FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
                     AFTDVMGMDELYK"
     CDS             3838..6849
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLVCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    6534..6651
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7423..7656
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7841..7885
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8179..8205
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(8490..8509)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8555..8610
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8609..8628)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8971..8987)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8971..8987)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8984..9006)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8995..9011
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9019..9025,9026..9043,9044..9049))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9064..9085
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9144..9339
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9190..9325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9345..9479
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9382..9401)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9436..9455
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9547..9564)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9718..10306)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9798..9817)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10477..11268,11269..11337))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11100..11119
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11338..11442)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgggccgcg
     1741 ttgacgcgca cggcaagagg cgaggggcgg cgactggtga gagatgggtg cgagagcgtc
     1801 agtattaagc gggggagaat tagatcgatg ggaaaaaatt cggttaaggc cagggggaaa
     1861 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     1921 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     1981 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     2041 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     2101 agagcaaaac aaaagtaaga aaaaagcaca gcaagcagca gctgacacag gacacagcaa
     2161 tcaggtcagc caaaattacc ctatagtgca gaacatccag gggcaaatgg tacatcaggc
     2221 catatcacct agaactttaa atgcatgggt aaaagtagta gaagagaagg ctttcagccc
     2281 agaagtgata cccatgtttt cagcattatc agaaggagcc accccacaag atttaaacac
     2341 catgctaaac acagtggggg gacatcaagc agccatgcaa atgttaaaag agaccatcaa
     2401 tgaggaagct gcagaatggg atagagtgca tccagtgcat gcagggccta ttgcaccagg
     2461 ccagatgaga gaaccaaggg gaagtgacat agcaggaact actagtaccc ttcaggaaca
     2521 aataggatgg atgacacata atccacctat cccagtagga gaaatctata aaagatggat
     2581 aatcctggga ttaaataaaa tagtaagaat gtatagccct accagcattc tggacataag
     2641 acaaggacca aaggaaccct ttagagacta tgtagaccga ttctataaaa ctctaagagc
     2701 cgagcaagct tcacaagagg taaaaaattg gatgacagaa accttgttgg tccaaaatgc
     2761 gaacccagat tgtaagacta ttttaaaagc attgggacca ggagcgacac tagaagaaat
     2821 gatgacagca tgtcagggag tggggggacc cggccataaa gcaagagttt tggctgaagc
     2881 aatgagccaa gtaacaaatc cagctaccat aatgatacag aaaggcaatt ttaggaacca
     2941 aagaaagact gttaagtgtt tcaattgtgg caaagaaggg cacatagcca aaaattgcag
     3001 ggcccctagg aaaaagggct gttggaaatg tggaaaggaa ggacaccaaa tgaaagattg
     3061 ttccggtgga ggtggatccg gtggaggttc catcgatgtg agcaagggcg aggaggataa
     3121 catggcctct ctcccagcga cacatgagtt acacatcttt ggctccatca acggtgtgga
     3181 ctttgacatg gtgggtcagg gcaccggcaa tccaaatgat ggttatgagg agttaaacct
     3241 gaagtccacc aagggtgacc tccagttctc cccctggatt ctggtccctc atatcgggta
     3301 tggcttccat cagtacctgc cctaccctga cgggatgtcg cctttccagg ccgctatggt
     3361 agatggctcc ggataccaag tccatcgcac aatgcagttt gaagatggtg cctcccttac
     3421 tgttaactac cgctacacct acgagggaag ccacatcaaa ggagaggccc aggtgaaggg
     3481 gactggtttc cctgctgacg gtcctgtgat gaccaactcg ctgaccgctg cggactggtg
     3541 caggtcgaag aagacttacc ccaacgacaa aaccatcatc agtaccttta agtggagtta
     3601 caccactgga aatggcaagc gctaccggag cactgcgcgg accacctaca cctttgccaa
     3661 gccaatggcg gctaactatc tgaagaacca gccgatgtac gtgttccgta agacggagct
     3721 caagcactcc aagaccgagc tcaacttcaa ggagtggcaa aaggccttta ccgatgtgat
     3781 gggcatggac gagctgtaca agtccggagg tggaggtgga actgagagac aggctaattt
     3841 tttagggaag atctggcctt cccacaaggg aaggccaggg aattttcttc agagcagacc
     3901 agagccaaca gccccaccag aagagagctt caggtttggg gaagagacaa caactccctc
     3961 tcagaagcag gagccgatag acaaggaact gtatccttta gcttccctca gatcactctt
     4021 tggcagcgac ccctcgtcac aataaagata ggggggcaat taaaggaagc tctattagat
     4081 acaggagcag atgatacagt attagaagaa atgaatttgc caggaagatg gaaaccaaaa
     4141 atgatagggg gaattggagg ttttatcaaa gtaagacagt atgatcagat actcatagaa
     4201 atctgcggac ataaagctat aggtacagta ttagtaggac ctacacctgt caacataatt
     4261 ggaagaaatc tgttgactca gattggctgc actttaaatt ttcccattag tcctattgag
     4321 actgtaccag taaaattaaa gccaggaatg gatggcccaa aagttaaaca atggccattg
     4381 acagaagaaa aaataaaagc attagtagaa atttgtacag aaatggaaaa ggaaggaaaa
     4441 atttcaaaaa ttgggcctga aaatccatac aatactccag tatttgccat aaagaaaaaa
     4501 gacagtacta aatggagaaa attagtagat ttcagagaac ttaataagag aactcaagat
     4561 ttctgggaag ttcaattagg aataccacat cctgcagggt taaaacagaa aaaatcagta
     4621 acagtactgg atgtgggcga tgcatatttt tcagttccct tagataaaga cttcaggaag
     4681 tatactgcat ttaccatacc tagtataaac aatgagacac cagggattag atatcagtac
     4741 aatgtgcttc cacagggatg gaaaggatca ccagcaatat tccagtgtag catgacaaaa
     4801 atcttagagc cttttagaaa acaaaatcca gacatagtca tctatcaata catggatgat
     4861 ttgtatgtag gatctgactt agaaataggg cagcatagaa caaaaataga ggaactgaga
     4921 caacatctgt tgaggtgggg atttaccaca ccagacaaaa aacatcagaa agaacctcca
     4981 ttcctttgga tgggttatga actccatcct gataaatgga cagtacagcc tatagtgctg
     5041 ccagaaaagg acagctggac tgtcaatgac atacagaaat tagtgggaaa attgaattgg
     5101 gcaagtcaga tttatgcagg gattaaagta aggcaattat gtaaacttct taggggaacc
     5161 aaagcactaa cagaagtagt accactaaca gaagaagcag agctagaact ggcagaaaac
     5221 agggagattc taaaagaacc ggtacatgga gtgtattatg acccatcaaa agacttaata
     5281 gcagaaatac agaagcaggg gcaaggccaa tggacatatc aaatttatca agagccattt
     5341 aaaaatctga aaacaggaaa gtatgcaaga atgaagggtg cccacactaa tgatgtgaaa
     5401 caattaacag aggcagtaca aaaaatagcc acagaaagca tagtaatatg gggaaagact
     5461 cctaaattta aattacccat acaaaaggaa acatgggaag catggtggac agagtattgg
     5521 caagccacct ggattcctga gtgggagttt gtcaataccc ctcccttagt gaagttatgg
     5581 taccagttag agaaagaacc cataatagga gcagaaactt tctatgtaga tggggcagcc
     5641 aatagggaaa ctaaattagg aaaagcagga tatgtaactg acagaggaag acaaaaagtt
     5701 gtccccctaa cggacacaac aaatcagaag actgagttac aagcaattca tctagctttg
     5761 caggattcgg gattagaagt aaacatagtg acagactcac aatatgcatt gggaatcatt
     5821 caagcacaac cagataagag tgaatcagag ttagtcagtc aaataataga gcagttaata
     5881 aaaaaggaaa aagtctacct ggcatgggta ccagcacaca aaggaattgg aggaaatgaa
     5941 caagtagata aattggtcag tgctggaatc aggaaagtac tatttttaga tggaatagat
     6001 aaggcccaag aagaacatga gaaatatcac agtaattgga gagcaatggc tagtgatttt
     6061 aacctaccac ctgtagtagc aaaagaaata gtagccagct gtgataaatg tcagctaaaa
     6121 ggggaagcca tgcatggaca agtagactgt agcccaggaa tatggcagct agtttgtaca
     6181 catttagaag gaaaagttat cttggtagca gttcatgtag ccagtggata tatagaagca
     6241 gaagtaattc cagcagagac agggcaagaa acagcatact tcctcttaaa attagcagga
     6301 agatggccag taaaaacagt acatacagac aatggcagca atttcaccag tactacagtt
     6361 aaggccgcct gttggtgggc ggggatcaag caggaatttg gcattcccta caatccccaa
     6421 agtcaaggag taatagaatc tatgaataaa gaattaaaga aaattatagg acaggtaaga
     6481 gatcaggctg aacatcttaa gacagcagta caaatggcag tattcatcca caattttaaa
     6541 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     6601 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     6661 tacagggaca gcagagatcc agtttggaaa ggaccagcaa agctcctctg gaaaggtgaa
     6721 ggggcagtag taatacaaga taatagtgac ataaaagtag tgccaagaag aaaagcaaag
     6781 atcatcaggg attatggaaa acagatggca ggtgatgatt gtgtggcaag tagacaggat
     6841 gaggattaac acatggaatt ctgcaacaac tgctgtttat ccatttcaga attgggtgtc
     6901 gacatagcag aataggcgtt actcgacaga ggagagcaag aaatggagcc agtagatcct
     6961 agactagagc cctggaagca tccaggaagt cagcctaaaa ctgcttgtac caattgctat
     7021 tgtaaaaagt gttgctttca ttgccaagtt tgtttcatga caaaagcctt aggcatctcc
     7081 tatggcagga agaagcggag acagcgacga agagctcatc agaacagtca gactcatcaa
     7141 gcttctctat caaagcagta agtagtacat gtaatgcaac ctataatagt agcaatagta
     7201 gcattagtag tagcaataat aatagcaata gttgtgtggt ccatagtaat catagaatat
     7261 aggaaaatgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     7321 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     7381 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     7441 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     7501 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     7561 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     7621 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     7681 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     7741 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     7801 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     7861 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     7921 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     7981 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     8041 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     8101 cagagacaga tccattcgat tagtgaacgg atccttggca cttatctggg acgatctgcg
     8161 gagcctgtgc ctcttcagct accaccgctt gagagactta ctcttgattg taacgaggat
     8221 tgtggaactt ctgggacgca gggggtggga agccctcaaa tattggtgga atctcctaca
     8281 atattggagt caggagctaa agaatagtgc tgttagcttg ctcaatgcca cagccatagc
     8341 agtagctgag gggacagata gggttataga agtagtacaa ggagcttgta gagctattcg
     8401 ccacatacct agaagaataa gacagggctt ggaaaggatt ttgctataag ctcgaaacaa
     8461 ccggtacctc tagaactata gctagcagat ctttttccct ctgccaaaaa ttatggggac
     8521 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     8581 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     8641 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     8701 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     8761 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     8821 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     8881 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     8941 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     9001 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9061 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     9121 aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta
     9181 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9241 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9301 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag
     9361 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9421 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     9481 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     9541 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     9601 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     9661 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     9721 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9781 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9841 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9901 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9961 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
    10021 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
    10081 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
    10141 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
    10201 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    10261 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    10321 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    10381 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    10441 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    10501 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    10561 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    10621 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    10681 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    10741 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    10801 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10861 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10921 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10981 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    11041 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    11101 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    11161 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    11221 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    11281 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    11341 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    11401 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    11461 gccacctg
//
