LOCUS       sequence-386218-       10284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sars-CoV-2 S_Y144del in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10284)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 10284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 S_Y144del
FEATURES             Location/Qualifiers
     source          1..10284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(110..127)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(281..869)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(361..380)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1040..1831,1832..1900))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1663..1682
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1901..2005)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2080..2099)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2271..2650
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2651..2854
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2805..2825
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2877..2895
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2905..2929
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3072..3096
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6913..6937)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6984..7007
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7044..7067
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7188..7211
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7242..7268
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             7242..7268
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(7269..7616,7617..7619,7620..8228)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(8272..8289)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8278..8502
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8635..8654)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8786..8833
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8841..9863
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8841..9863
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9993..10114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10030..10049)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10084..10103
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10163..10179)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10163..10179)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10176..10198)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10187..10203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10211..10217,10218..10235,10236..10241))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10256..10277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
       61 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      121 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      181 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      241 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      301 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      361 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      421 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      481 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      541 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      601 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      661 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      721 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      781 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      841 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      901 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      961 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1021 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1081 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1141 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1201 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1261 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1321 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1381 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1441 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1501 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1561 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1621 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1681 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1741 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1801 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1861 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1921 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1981 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     2041 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     2101 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     2161 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     2221 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
     2281 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     2341 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2401 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2461 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2521 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2581 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2641 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2701 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2761 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2821 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
     2881 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
     2941 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     3001 agcgtttaaa cttaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc
     3061 tgcagatatc aacaagtttg tacaaaaaag ttggcatgtt cgtgttcctg gtgctgctgc
     3121 cactggtgag ctcccagtgc gtgaacctga ccaccagaac ccagctgcca cctgcctaca
     3181 ccaactcctt caccaggggc gtgtactacc cagacaaggt gttcagatcc agcgtgctgc
     3241 actctaccca ggacctgttc ctgccattct tctctaacgt gacctggttc cacgccatcc
     3301 acgtgtctgg aaccaacggc accaagagat tcgacaaccc agtgctgcca ttcaacgacg
     3361 gcgtgtactt cgcttctacc gagaagtcta acatcatcag gggttggatc ttcggaacca
     3421 ccctggactc taagacccag tctctgctga tcgtgaacaa cgctaccaac gtggtgatca
     3481 aggtgtgcga gttccagttc tgcaacgacc cattcctggg agtgtaccac aagaacaaca
     3541 agtcttggat ggaaagcgag ttcagggtgt acagctctgc taacaactgc accttcgagt
     3601 acgtgtctca gcctttcctg atggacctgg aaggaaagca gggaaacttc aagaacctga
     3661 gagagttcgt gttcaagaac atcgacggtt acttcaagat atacagcaag cacaccccaa
     3721 tcaacctggt gagggacctg ccacagggtt tctctgctct ggagcccctg gtggacctgc
     3781 caatcggcat caacatcacc aggttccaga ccctgctggc tctgcacagg tcttacctga
     3841 ccccaggcga cagcagctct ggatggaccg ccggcgccgc tgcttactac gtgggatacc
     3901 tgcagccaag gaccttcctg ctgaagtaca acgaaaacgg caccatcacc gacgccgtgg
     3961 actgcgctct ggacccactg tctgagacca agtgcaccct gaagtctttc accgtggaaa
     4021 agggaatcta ccagaccagc aacttccgcg tgcagcccac cgagtctatc gtgagattcc
     4081 caaacatcac caacctgtgc ccattcggag aagtgttcaa cgctaccagg ttcgccagcg
     4141 tgtacgcttg gaacaggaag agaatcagca actgcgtggc cgactactct gtgctgtaca
     4201 actctgcctc cttctccacc ttcaagtgct acggcgtgtc cccaaccaag ctgaacgacc
     4261 tgtgcttcac caacgtgtac gctgactcct tcgtgatcag aggagacgaa gtgagacaga
     4321 tcgctcccgg tcagaccgga aagatcgccg actacaacta caagctgcca gacgacttca
     4381 ccggttgcgt gatcgcttgg aactctaaca acctggactc taaggtggga ggcaactaca
     4441 actacctgta caggctgttc cgcaagagca acctgaagcc tttcgaaagg gacatcagca
     4501 ccgaaatcta ccaggctgga tctaccccat gcaacggagt ggaaggattc aactgctact
     4561 tccctctgca gtcttacgga ttccagccaa ccaacggagt gggataccag ccatacagag
     4621 tggtggtgct gtctttcgag ctgctgcacg ctcctgctac cgtgtgcgga ccaaagaagt
     4681 ctaccaacct ggtgaagaac aagtgcgtga acttcaactt caacggactg accggaaccg
     4741 gagtgctgac cgaatccaac aagaagttcc tgcctttcca gcagttcggt agagacatcg
     4801 ccgacaccac cgacgctgtg agagacccac agaccctgga aatcctggac atcaccccct
     4861 gctctttcgg aggagtgagc gtgatcaccc caggaaccaa cacctccaac caggtggccg
     4921 tgctgtacca ggacgtgaac tgcaccgaag tgccagtggc catccacgct gaccagctga
     4981 ccccaacctg gagagtgtac agcaccggat ctaacgtgtt ccagacccgt gccggctgcc
     5041 tgatcggagc cgagcacgtg aacaactcct acgaatgcga catcccaatc ggagctggca
     5101 tctgcgcttc ttaccagacc cagaccaaca gcccaagaag ggccagatcc gtggcctctc
     5161 agagcatcat cgcctacacc atgagcctgg gcgctgaaaa cagcgtggct tacagcaaca
     5221 actctatcgc catcccaacc aacttcacca tctctgtgac caccgaaatc ctgccagtga
     5281 gcatgaccaa gaccagcgtg gactgcacca tgtacatctg cggagactct accgaatgct
     5341 ctaacctgct gctgcagtac ggcagcttct gcacccagct gaaccgcgct ctgaccggaa
     5401 tcgctgtgga gcaggacaag aacacccagg aagtgttcgc tcaggtgaag cagatataca
     5461 agaccccacc tatcaaggac ttcggaggat tcaacttctc tcagatcctg cccgacccat
     5521 ctaagccctc taagaggtct ttcatcgaag acctgctgtt caacaaggtg accctggccg
     5581 acgccggttt catcaagcag tacggtgact gcctgggaga catcgccgct agagacctga
     5641 tctgcgccca gaagttcaac ggtctgaccg tgctgccacc actgctgacc gacgagatga
     5701 tcgcccagta cacctctgcc ctgctggctg gaaccatcac ctctggttgg accttcggag
     5761 ctggcgccgc cctgcagatc ccattcgcta tgcagatggc ctaccgtttc aacggtatcg
     5821 gagtgaccca gaacgtgctg tacgaaaacc agaagctgat cgctaaccag ttcaactctg
     5881 ctatcggaaa gatccaggac tccctgtcct ctaccgcttc tgccctggga aagctgcagg
     5941 acgtggtgaa ccagaacgcc caggccctga acaccctggt gaagcagctg tcttctaact
     6001 tcggagccat ctcctctgtg ctgaacgaca tcctgtctag gctggacaag gtggaagccg
     6061 aggtgcagat cgacagactg atcaccggaa gactgcagtc tctgcagacc tacgtgaccc
     6121 agcagctgat cagggccgct gaaatcagag cttctgccaa cctggctgcc accaagatgt
     6181 ccgaatgcgt gctgggccag tctaagaggg tggacttctg cggaaaggga taccacctga
     6241 tgtccttccc acagtctgct ccacacggag tggtgttcct gcacgtgacc tacgtgcccg
     6301 cccaggaaaa gaacttcacc accgccccag ctatctgcca cgacggaaag gctcacttcc
     6361 ccagggaggg agtgttcgtg tctaacggaa cccactggtt cgtgacccag aggaacttct
     6421 acgagccaca gatcatcacc accgacaaca ccttcgtgtc tggaaactgc gacgtggtga
     6481 tcggaatcgt gaacaacacc gtgtacgacc cactgcagcc agaactggac tctttcaagg
     6541 aggaactgga caagtacttc aagaaccaca cctctccaga cgtggacctg ggagacatct
     6601 ccggaatcaa cgcctctgtg gtgaacatcc agaaggaaat cgacagactg aacgaggtgg
     6661 ccaagaacct gaacgaatct ctgatcgacc tgcaggaact gggaaagtac gagcagtaca
     6721 tcaagtggcc ctggtacatc tggctgggtt tcatcgctgg tctgatcgct atcgtgatgg
     6781 tgaccatcat gctgtgctgc atgacctctt gctgctcttg cctgaaggga tgctgctctt
     6841 gcggatcttg ctgcaagttc gacgaggacg actccgagcc tgtgctgaag ggtgtgaagc
     6901 tgcactacac ctacccaact ttcttgtaca aagtggttga tatcaaggcc gccgacatca
     6961 ccagcctgta caagaaagtg gggtggagcc atccccagtt cgagaagggc ggaggatctg
     7021 gcggcggaag cggcggagga agctggtccc accctcagtt tgagaaaggc accgagctgg
     7081 gcagcaccat ggccagctac ccctacgacs tgymcraswa mgysrrgkrs agccatcccc
     7141 agttcgagaa gggcggagga tctggcggcg gaagcggcgg aggaagctgg tcccaccctc
     7201 agtttgagaa aggcaccgag ctgggcagca ccatggccag ctacccctac gacgtgcccg
     7261 actacgccaa ggacaacacc gtgcccctga agctgatcgc cctgctggcc aacggcgagt
     7321 tccactctgg cgagcagctg ggcgagacac tgggcatgag cagagccgcc atcaacaagc
     7381 acatccagac cctgcgggac tggggcgtgg acgtgttcac agtgcccggc aagggctaca
     7441 gcctgcccga gcccatccag ctgctgaacg ccaagcagat cctgggccag ctggatggcg
     7501 gatctgtggc cgtgctgccc gtgatcgaca gcaccaacca gtatctgctc gaccggatcg
     7561 gcgagctgaa gtccggcgac gcctgtatcg ccgagtacca gcaggccggc agaggcggca
     7621 ggggcagaaa gtggttcagc cccttcggcg ccaacctgta cctgagcatg ttctggagac
     7681 tggaacaggg cccagccgcc gctattggcc tgagcctggt catcggcatc gtgatggccg
     7741 aggtgctgcg gaagctgggc gccgacaaag tgcgcgtgaa gtggcccaac gacctgtatc
     7801 tgcaggaccg gaagctggcc ggcatcctgg tggaactgac cggcaaaaca ggcgacgccg
     7861 cccagatcgt gatcggcgct ggcatcaaca tggccatgcg gcgggtggaa gagagcgtgg
     7921 tcaaccaggg ctggatcacc ctgcaggaag ccggcatcaa cctggaccgg aacaccctgg
     7981 ccgccatgct gatcagagag ctgagagccg ccctggaact gttcgagcag gaaggcctgg
     8041 ccccctacct gagcagatgg gagaagctgg acaacttcat caaccggccc gtgaaactga
     8101 tcatcggcga caaagagatc ttcggcatca gccggggcat cgacaagcag ggggccctgc
     8161 tcctggaaca ggacggcatc atcaagccct ggatgggcgg cgagatcagc ctgcggagcg
     8221 ccgagaagtg atgactcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg
     8281 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     8341 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     8401 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     8461 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     8521 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     8581 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     8641 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     8701 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     8761 attagggtga tggttcacgt acctagaagt tcctattccg aagttcctat tctctagaaa
     8821 gtataggaac ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     8881 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     8941 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     9001 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     9061 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
     9121 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     9181 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     9241 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     9301 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     9361 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     9421 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     9481 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     9541 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     9601 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     9661 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     9721 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     9781 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     9841 ctcgtccgag ggcaaaggaa tagcacgtac tacgagattt cgattccacc gccgccttct
     9901 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     9961 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
    10021 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    10081 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
    10141 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    10201 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    10261 tgagctaact cacattaatt gcgt
//
