LOCUS       sequence-386296-       10308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSwap plasmid part with H2_beta homology arms and chloramphenicol 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Teufel M, Klein CA, Mager M, Sobetzko P
  TITLE     A multifunctional system for genome editing and large-scale 
            interspecies gene transfer.
  JOURNAL   Nat Commun. 2022 Jun 14;13(1):3430. doi: 10.1038/s41467-022-30843-1.
  PUBMED    35701417
REFERENCE   2  (bases 1 to 10308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-06-14"; volume: "13(1):3430. doi"; pages: " 
            10.1038/s41467-022-30843-1"
COMMENT     Sequence Label: pSWAP_Entry_H2_beta_CM
FEATURES             Location/Qualifiers
     source          1..10308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            157..265
                     /note="incP origin of transfer"
     protein_bind    375..393
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    400..418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        449..524
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      544..614
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      1454..1673
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             1764..2519
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    2522..2772
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             3098..4273
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             4273..5244
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    5317..5790
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     rep_origin      6249..6837
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6738..6757
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(7811..7845)
                     /label=BBa_J23116
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.16"
     protein_bind    7992..8013
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8028..8033,8034..8051,8052..8058)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8066..8082
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8071..8093
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8090..8106
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8090..8106
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8102..8425
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    8119..8175
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8176..8193)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8176..8192)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8185..8207)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8401..8420)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             8767..9072
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8951..8970
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    9292..9310
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9317..9335
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        9366..9441
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      9461..9532
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(9579..10238)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     10153..10172
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(10257..10304)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 caccgctagc ccttaaggta tactttccgc tgcataaccc tgcttcgggg tcattatagc
       61 gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg
      121 tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc
      181 acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg
      241 gaatcctgct ctgcgaggct ggccgataag ctcccgggtg ctcgatgttg ttcagaacgc
      301 tcggtcttgc acaccgggcg ttttttcttt gtgagtccag gcaagctttc gctaaggatg
      361 atttctggaa ttcttcccta tcagtgatag agattgacat ccctatcagt gatagagata
      421 ctgagcacaa ccaaggtcca aacggtgagt tttagagcta gaaatagcaa gttaaaataa
      481 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tcaagccagg
      541 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      601 tcggtgaacg ctctggatat attccgcttc ctcgctcact cgatggagcg gcgtaaccgt
      661 cgcacaggaa ggacagagaa agcgcggatc tgggaagtga cggacagaac ggtcaggacc
      721 tggattgggg aggcggttgc cgccgctgct gctgacggtg tgacgttctc tgttccggtc
      781 acaccacata cgttccgcca ttcctatgcg atgcacatgc tgtatgccgg tataccgctg
      841 aaagttctgc aaagcctgat gggacataag tccatcagtt caacggaagt ctacacgaag
      901 gtttttgcgc tggatgtggc tgcccggctc cgggtgcagt ttgcgatgcc ggagtctgat
      961 gcggttgcga tgctgaaaca attatcctga gaataaatgc cttggccttt atatggaaat
     1021 gtggaactga gtggatatgc tgtttttgtc tgttaaacag agaagctggc tgttatccac
     1081 tgagaagcga acgaaacagt cgggaaaatc tcccattatc gtagagatcc gcattattaa
     1141 tctcaggagc ctgtgtagcg tttataggaa gtagtgttct gtcatgatgc ctgcaagcgg
     1201 taacgaaaac gatttgaata tgccttcagg aacaatagaa atcttcgtgc ggtgttacgt
     1261 tgaagtggag cggattatgt cagcaatgga cagaacaacc taatgaacac agaaccatga
     1321 tgtggtctgt ccttttacag ccagtagtgc tcgccgcagt cgagcgacag ggcgaagccc
     1381 tcgagtgagc gaggaagcac cagggaacag cacttatata ttctgcttac acacgatgcc
     1441 tgaaaaaact tcccttgggg ttatccactt atccacgggg atatttttat aattattttt
     1501 tttatagttt ttagatcttc ttttttagag cgccttgtag gcctttatcc atgctggttc
     1561 tagagaaggt gttgtgacaa attgcccttt cagtgtgaca aatcaccctc aaatgacagt
     1621 cctgtctgtg acaaattgcc cttaaccctg tgacaaattg ccctcagaag aagctgtttt
     1681 ttcacaaagt tatccctgct tattgactct tttttattta gtgtgacaat ctaaaaactt
     1741 gtcacacttc acatggatct gtcatggcgg aaacagcggt tatcaatcac aagaaacgta
     1801 aaaatagccc gcgaatcgtc cagtcaaacg acctcactga ggcggcatat agtctctccc
     1861 gggatcaaaa acgtatgctg tatctgttcg ttgaccagat cagaaaatct gatggcaccc
     1921 tacaggaaca tgacggtatc tgcgagatcc atgttgctaa atatgctgaa atattcggat
     1981 tgacctctgc ggaagccagt aaggatatac ggcaggcatt gaagagtttc gcggggaagg
     2041 aagtggtttt ttatcgccct gaagaggatg ccggcgatga aaaaggctat gaatcttttc
     2101 cttggtttat caaacgtgcg cacagtccat ccagagggct ttacagtgta catatcaacc
     2161 catatctcat tcccttcttt atcgggttac agaaccggtt tacgcagttt cggcttagtg
     2221 aaacaaaaga aatcaccaat ccgtatgcca tgcgtttata cgaatccctg tgtcagtatc
     2281 gtaagccgga tggctcaggc atcgtgtctc tgaaaatcga ctggatcata gagcgttacc
     2341 agctgcctca aagttaccag cgtatgcctg acttccgccg ccgcttcctg caggtctgtg
     2401 ttaatgagat caacagcaga actccaatgc gcctctcata cattgagaaa aagaaaggcc
     2461 gccagacgac tcatatcgta ttttccttcc gcgatatcac ttccatgacg acaggatagt
     2521 ctgagggtta tctgtcacag atttgagggt ggttcgtcac atttgttctg acctactgag
     2581 ggtaatttgt cacagttttg ctgtttcctt cagcctgcat ggattttctc atactttttg
     2641 aactgtaatt tttaaggaag ccaaatttga gggcagtttg tcacagttga tttccttctc
     2701 tttcccttcg tcatgtgacc tgatatcggg ggttagttcg tcatcattga tgagggttga
     2761 ttatcacagt ttattactct gaattggcta tccgcgtgtg tacctctacc tggagttttt
     2821 cccacggtgg atatttcttc ttgcgctgag cgtaagagct atctgacaga acagttcttc
     2881 tttgcttcct cgccagttcg ctcgctatgc tcggttacac ggctgcggcg agcgctagtg
     2941 ataataagtg actgaggtat gtggtgttct tatctccttt tgtagtgttg ctcttatttt
     3001 aaacaacttt gcggtttttt gatgactttg cgattttgtt gttgctttgc agtaaattgc
     3061 aagatttaat aaaaaaacgc aaagcaatga ttaaaggatg ttcagaatga aactcatgga
     3121 aacacttaac cagtgcataa acgctggtca tgaaatgacg aaggctatcg ccattgcaca
     3181 gtttaatgat gacagcccgg aagcgaggaa aataacccgg cgctggagaa taggtgaagc
     3241 agcggattta gttggggttt cttctcaggc tatcagagat gccgagaaag cagggcgact
     3301 accgcacccg gatatggaaa ttcgaggacg ggttgagcaa cgtgttggtt atacaattga
     3361 acaaattaat catatgcgtg atgtgtttgg tacgcgattg cgacgtgctg aggacgtatt
     3421 tccaccggtg atcggggttg ctgcccataa aggtggcgtt tacaaaacct cagtttctgt
     3481 tcatcttgct caggatctgg ctctgaaggg gctacgtgtt ttgctcgtgg aaggtaacga
     3541 cccccaggga acagcctcaa tgtatcacgg atgggtacca gatcttcata ttcatgcaga
     3601 agatactctc ctgcctttct atcttgggga aaaggacgat gtcacttatg caataaagcc
     3661 cacttgctgg ccggggcttg acattattcc ttcctgtctg gctctgcacc gtattgaaac
     3721 tgagttaatg ggcaaatttg atgaaggtaa actgcccacc gatccacacc tgatgctccg
     3781 actggccatt gaaactgttg ctcatgacta tgatgtcata gttattgaca gcgcgcctaa
     3841 cctgggtatc ggcacgatta atgtcgtatg tgctgctgat gtgctgattg ttcccacgcc
     3901 tgctgagttg tttgactaca cctccgcact gcagtttttc gatatgcttc gtgatctgct
     3961 caagaacgtt gatcttaaag ggttcgagcc tgatgtacgt attttgctta ccaaatacag
     4021 caatagtaat ggctctcagt ccccgtggat ggaggagcaa attcgggatg cctggggaag
     4081 catggttcta aaaaatgttg tacgtgaaac ggatgaagtt ggtaaaggtc agatccggat
     4141 gagaactgtt tttgaacagg ccattgatca acgcagttca actggtgcct ggagaaatgc
     4201 tctttctatt tgggaacctg tctgcaatga aattttcgat cgtctgatta aaccacgctg
     4261 ggagattaga taatgaagcg tgcgcctgtt attccaaaac atacgctcaa tactcaaccg
     4321 gttgaagata cttcgttatc gacaccagct gccccgatgg tggattcgtt aattgcgcgc
     4381 gtaggagtaa tggctcgcgg taatgccatt actttgcctg tatgtggtcg ggatgtgaag
     4441 tttactcttg aagtgctccg gggtgatagt gttgagaaaa cctctcgggt atggtcaggt
     4501 aatgaacgtg accaggagct gcttactgag gacgcactgg atgatctcat cccttctttt
     4561 ctactgactg gtcaacagac accggcgttc ggtcgaagag tatctggtgt catagaaatt
     4621 gccgatggga gtcgccgtcg taaagctgct gcacttaccg aaagtgatta tcgtgttctg
     4681 gttggcgagc tggatgatga gcagatggct gcattatcca gattgggtaa cgattatcgc
     4741 ccaacaagtg cttatgaacg tggtcagcgt tatgcaagcc gattgcagaa tgaatttgct
     4801 ggaaatattt ctgcgctggc tgatgcggaa aatatttcac gtaagattat tacccgctgt
     4861 atcaacaccg ccaaattgcc taaatcagtt gttgctcttt tttctcaccc cggtgaacta
     4921 tctgcccggt caggtgatgc acttcaaaaa gcctttacag ataaagagga attacttaag
     4981 cagcaggcat ctaaccttca tgagcagaaa aaagctgggg tgatatttga agctgaagaa
     5041 gttatcactc ttttaacttc tgtgcttaaa acgtcatctg catcaagaac tagtttaagc
     5101 tcacgacatc agtttgctcc tggagcgaca gtattgtata agggcgataa aatggtgctt
     5161 aacctggaca ggtcacgtgt tccaactgag tgtatagaga aaattgaggc cattcttaag
     5221 gaacttgaaa agccagcacc ctgatgcgac cacgttttag tctacgttta tctgtcttta
     5281 cttaatgtcc tttgttacag gccagaaagc ataactggcc tgaatattct ctctgggccc
     5341 actgttccac ttgtatcgtc ggtctgataa tcagactggg accacggtcc cactcgtatc
     5401 gtcggtctga ttattagtct gggaccacgg tcccactcgt atcgtcggtc tgattattag
     5461 tctgggacca cggtcccact cgtatcgtcg gtctgataat cagactggga ccacggtccc
     5521 actcgtatcg tcggtctgat tattagtctg ggaccatggt cccactcgta tcgtcggtct
     5581 gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctggaac
     5641 cacggtccca ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat
     5701 cgtcggtctg attattagtc tgggaccacg atcccactcg tgttgtcggt ctgattatcg
     5761 gtctgggacc acggtcccac ttgtattgtc gatcagacta tcagcgtgag actacgattc
     5821 catcaatgcc tgtcaagggc aagtattgac atgtcgtcgt aacctgtaga acggagtaac
     5881 ctcggtgtgc ggttgtatgc ctgctgtgga ttgctgctgt gtcctgctta tccacaacat
     5941 tttgcgcacg gttatgtgga caaaatacct ggttacccag gccgtgccgg cacgttaacc
     6001 gggctgcatc cgatgcaagt gtgtcgctgt cgacctgctc ggatctgttg gaccggacag
     6061 tagtcatggt tccctcgtct tccagaccaa gtttactcat atatacttta gattgattta
     6121 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6181 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6241 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6301 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6361 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6421 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6481 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6541 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6601 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6661 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6721 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6781 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6841 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6901 tttcctgcgt tatcccctgg aagacattgg tcgccgaaaa ccccgcttcg gcggggtttt
     6961 gccgcgctag taccaagagc gataaaacga aaggcccagt ctttctactg agcctttcgt
     7021 tttataagct tatttcagga aagtttcgga ggagatagtg ttcggcagtt tgtacatcat
     7081 ctgcgggatc aggtacggtt tgatcaggtt gtagaagatc aggtaagaca tagaatcgat
     7141 gtagatgatc ggtttgtttt tgttgatttt tacgtaacag ttcagttgga atttgttacg
     7201 cagaccctta accaggtatt ctacttcttc gaaagtgaaa gactgggtgt tcagtacgat
     7261 cgatttgttg gtagagtttt tgttgtaatc ccatttacca ccatcatcca tgaaccagta
     7321 tgccagagac atcggggtca ggtagttttc aaccaggttg ttcgggatgg tttttttgtt
     7381 gttaacgatg aacaggttag ccagtttgtt gaaagcttgg tgtttgaaag tctgggcgcc
     7441 ccaggtgatt accaggttac ccaggtggtt aacacgttct tttttgtgcg gcggggacag
     7501 tacccactga tcgtacagca gacatacgtg gtccatgtat gctttgtttt tccactcgaa
     7561 ctgcatacag taggttttac cttcatcacg agaacggatg taagcatcac ccaggatcag
     7621 accgatacct gcttcgaact gttcgatgtt cagttcgatc agctgggatt tgtattcttt
     7681 cagcagttta gagttcggac ccaggttcat tacctggttt tttttgatgt tatgcatttg
     7741 atctttagtg atagacacca tattgttcct ccttatggat ctgataaatt gttaaagagc
     7801 agtagtgttt gctagcatag tccctaggac tgagctagct gtcaatactg attcaggcta
     7861 tcaatatttg tcgctgcaag gtagctgatc gaaccaaggt ccaaacggtg acggggagtg
     7921 agacccagtg gtatgggcag ctggcacgac aggtttccct actggaaagc gggcagtgag
     7981 tgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     8041 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     8101 tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg actctagagg atccccgggt
     8161 accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     8221 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     8281 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat
     8341 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     8401 tacaatctgc tctgatgccg catagtcgcg tggatccggc ttactaaaag ccagataaca
     8461 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg
     8521 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac
     8581 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca
     8641 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga
     8701 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct
     8761 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg
     8821 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt
     8881 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat
     8941 gaaagctggc gcatgatgac caccgatatg gccagtgtgc ctgtctccgt tatcggggaa
     9001 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
     9061 tggggaatat aattaagcca gccccgacac ccgccaacac ccactgacgc gccctgacgg
     9121 gcttgtctgc tcccggcatc cgcttacaga caagggtctc gcgctctata ttcgctatcg
     9181 atcgtagcaa ttgtcaacag gctgttgttc agaacgctcg gtcttgcaca ccgggcgttt
     9241 tttctttgtg agtccaggca agctttcgct aaggatgatt tctggaattc ttccctatca
     9301 gtgatagaga ttgacatccc tatcagtgat agagatactg agcacgatat gcccgtagct
     9361 actaggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
     9421 aaagtggcac cgagtcggtg ctttttttca agccaggcat caaataaaac gaaaggctca
     9481 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcaagtgagt
     9541 ctcacgctct gggttcaaaa cgtcgtgaga cagtttggtc acgccccgcc ctgccactca
     9601 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     9661 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     9721 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     9781 aaactcaccc agggattggc tgacacgaaa aacatattct caataaaccc tttagggaaa
     9841 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     9901 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     9961 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg
    10021 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
    10081 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
    10141 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
    10201 atatcaacgg tggtatatcc agtgattttt ttctccatgg tttagttcct caccttgtcg
    10261 tattatacta tgccgatata ctatgccgat gattaattgt caacaggc
//
