LOCUS       sequence-386315-        4296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human short-form UBASH3A lacking the UBA domain in Gateway pDONR221 
            (for N-terminal tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4296)
  TITLE     Concannon Lab Plasmids
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDONR221-deltaUBA-short-UBASH3A-Ntag
FEATURES             Location/Qualifiers
     source          1..4296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     168..187
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     421..438
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(597..624)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(716..802)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     851..873
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     865..882
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     866..882
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    898..997
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2750..2849)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2866..2885)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2867..2885)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2890..2906)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2890..2906)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3008..3027)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3019..3828
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3086..3105)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(3975..4296,1..267)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
       61 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      121 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      181 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      241 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      301 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      361 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
      421 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
      481 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
      541 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
      601 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
      661 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
      721 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
      781 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
      841 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa
      901 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc
      961 ttttttataa tgccaacttt gtacaaaaaa gcaggcttca tggcagcggg ggagacgcag
     1021 ctctacgcca aggtctccaa caagctcaag agccgctgca atgacccttc cctagacgac
     1081 cccatccccc aggagtatgc ccttttcctc tgtccaacgg ggcccctgct ggaaaaactt
     1141 caagagttct ggagagagag caagcgccag tgtgcaaaga acagagctca tgaggtcttc
     1201 ccacacgtga cactctgtga cttcttcacg tgtgaagacc agaaggtgga atgcctgtac
     1261 gaggcgctga agagagctgg agacaggctc ctgggctcct tccccacggc cgtgcctctg
     1321 gctctccact cctccatcag ctacctcggc ttcttcgtca gtggcagccc cgcagacgtc
     1381 atccgggaat tcgccatgac cttcgccacg gaagcatctc tcttagcaga ctgctccgtg
     1441 aagccttgca ccaaacagct gcatctgacc ttggcccaca agttctaccc ccaccaccag
     1501 aggacgctgg agcagctggc cagagccatc cccctgggcc acagctgcca gtggaccgca
     1561 gcactctact cccgagacat gcgctttgtg cactaccaga ccctgagagc cctattccag
     1621 tacaaacccc agaacgtgga tgagctgacg ctaagtcctg gtgactacat ctttgtggac
     1681 cccacgcagc aggacgaagc cagcgagggc tgggtgattg ggatctcaca gcggacgggc
     1741 tgccggggct tcctgccgga aaactacacg gatcgagcca gtgagtctga cacgtgggtg
     1801 aagcacagga tgtacacctt cagtctagcc acagacctga actccagaaa ggatggtgaa
     1861 gccagcagca gatgcagcgg ggaatttctt ccacaaacgg caaggagtct tagcagctta
     1921 caggccttgc aggctaccgt tgcaaggaag agtgtgctgg tggttcgcca cggggagaga
     1981 gtggatcaga tcttcgggaa ggcatggctg cagcaatgct ccactcctga tgggaaatac
     2041 tacaggccag acctgaattt cccctgcagt ctgcccagac ggagtcgtgg gatcaaagac
     2101 tttgaaaacg atcccccatt atcatcgtgt ggcattttcc agtccagaat tgcaggggac
     2161 gcgctactgg acagtggtat cagaatcagc tctgtgtttg cctccccagc cctccgctgt
     2221 gtgcagacgg ccaaactcat cctggaagaa ctcaaactgg agaaaaaaat caagatacga
     2281 gtggaacctg gaatctttga atggacaaaa tgggaagctg gcaagaccac cccaaccctc
     2341 atgagcctgg aagagctgaa agaggcaaat ttcaacattg acactgatta caggcccgcg
     2401 tttcccctgt ccgccctcat gccggccgag agctaccagg agtacatgga caggtgcacg
     2461 gcgagcatgg tgcaaatcgt caacacctgt ccgcaggaca cgggtgtcat cctaattgtg
     2521 agtcacggct ccactctgga ctcctgcacg cggccactgc tcgggctgcc gccccgggaa
     2581 tgtggggatt ttgcccaact cgtgagaaag atcccttccc tgggcatgtg cttctgtgaa
     2641 gaaaataaag aggaaggaaa atgggagttg gtgaacccac cggtgaagac cctgacccac
     2701 ggggcgaacg cagcatttaa ctggaggaac tggatctcag gcaactgaaa cccagctttc
     2761 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     2821 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     2881 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     2941 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     3001 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     3061 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     3121 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     3181 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     3241 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     3301 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     3361 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     3421 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     3481 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     3541 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     3601 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     3661 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     3721 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     3781 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     3841 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     3901 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     3961 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     4021 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     4081 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     4141 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     4201 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     4261 tagttaccgg ataaggcgca gcggtcgggc tgaacg
//
