LOCUS       sequence-386335-       12416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR, Cas9 driven by double 35S, nptII for plant selection, 
            knockout: CEN12 and Fuzzy3MYB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12416)
  AUTHORS   Ortega MA, Zhou R, Chen MSS, Bewg WP, Simon B, Tsai CJ
  TITLE     In vitro floral development in poplar: insights into seed trichome 
            regulation and trimonoecy.
  JOURNAL   New Phytol. 2022 Nov 16. doi: 10.1111/nph.18624.
  PUBMED    36385612
REFERENCE   2  (bases 1 to 12416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol.
            2022 Nov 16. doi: 10.1111/nph.18624."
COMMENT     Sequence Label: pORE303N_CEN12Fuzzy3MYB
FEATURES             Location/Qualifiers
     source          1..12416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(502..1296)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(613..632)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1223..1242
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_RNA        2845..2920
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3017..3693
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     3630..3650
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             3739..7842
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3739..7842
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3739..7842
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      7882..8130
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    8149..8173
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8387..8975
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8876..8895
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9129..9146
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(9215..10363)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10662..11456)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11668..12299
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    12379..12403
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaatggtagg cgcggcttaa ttaagcgctt aattaagcgc ttaattaagc gcttaataag
       61 ccgcgccgta ccccaatagt ctcgacagac acatagcacc taacattatt gcttaaattt
      121 actatttttt tactatatta tactcaaccc aatgagcata aagactgtaa aatctcaaat
      181 tcctgagaag catatttatc gatcccacag acttgatagt tccataatcc atacgctgca
      241 gccaaattgc tagtgtgttg aacatttaac acgtagagaa ctagaaaaga tataaaacta
      301 agattgatat ccaaaataga cgagaacaat aagcaaaaac tcttagtttt gaaataaatc
      361 aacaatcccg agggttgtca catacatcaa aaacgaaaat ccatatagca aaaaaaactc
      421 taaattaccg ttcgacaaaa agagaaaact gataggacat ttgctaaaca ttaaaatcaa
      481 aatttggatc aagccgaatt ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
      541 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
      601 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
      661 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
      721 gcaggcatcg ccatgggtca cgacgagatc atcgccgtcg ggcatgcgcg ccttgagcct
      781 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
      841 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
      901 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
      961 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     1021 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     1081 cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag
     1141 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     1201 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     1261 cggagaacct gcgtgcaatc catcttgttc aatcatctcg agacgtaccg cgcgatgcat
     1321 tcgaagatcg atccttcgcc tggaggagag aaatcagtgg cgctgcggct tttagggttt
     1381 ctttgttgat ggaatgagag tgtaagctct gccagtgcca ctttattagg gttttacaag
     1441 cccttttctt cgtaattggg cctgacattt tgtgccactt gggcctttag agatgaaaat
     1501 gtatattggg cttaagttga cttgaaggat aaattagttt aggatattac gttttttatg
     1561 agaattggtg tgtcggatac atgtatatga tgcattcaaa tatatgtatt ctagatacat
     1621 ttaagtttag atacaatcta aaatgtgtct ttaattacag gactgtaact aaaatactta
     1681 atgtaagaag aatattactc ctttaatagc ttttgagtat atctagtcta acatctttta
     1741 aaaaagtcta atttctttca tttatttttc gagcaatagc aaagtgcata attatttttt
     1801 tcttctagaa attcagattt gtttctctaa attttgagat tcttttctca attttgtatg
     1861 tctagagaac aatgtgtatt tttcactcta gttggttgtt actttgttga atgttctgat
     1921 aaaagtatat tgttatttct gaagtagata taaaccttca tttggaaatt atacataaat
     1981 caaaatcgtt aattatctag atcaagatat atgccctttt cctaatgtat ttgatacatg
     2041 cacctaattt cactagatgt atcttttcta ttttttaaat tatgaatagt taattttttc
     2101 catatgtgta tttgatacat acttcatgac tttaaaaaat taattatata ccagatatat
     2161 gtatttaaaa tttgttatgt atttaaagta tgtatatgat tattcgatat taatctcttc
     2221 gatgaaccta ggatgcctat cttatatgat caatgaggca tttaattggg tgcatatgat
     2281 ggtgaaaaaa ggtgcagctc ctggcttggg aatgatgact catgtggaat ttggtcttaa
     2341 atttatcaca tccttttggg atgtgatgat tgtatcactt gttcattttg caaagacaag
     2401 gtgcactgct acaaactttg gtttaatctg aaataaaaca aaactcactg agaggaagat
     2461 gcatcccagt aggtgaaagt cgagaaggat ttgcatgtta ctattacact tgctttttag
     2521 tcccacatcg tctgaaacat aaaatatttc agcgtttaaa tacttcaagc gaaccagtag
     2581 gcttgcttgt ggttgggaga gtgatgtttc agagctatgc tggaaacagc atagcaagtt
     2641 gaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttttg
     2701 ctacctcagc atagtctaca gcataagctt atgatttctt ttttcttacg aattttgcgt
     2761 cccacatcgg taagcgagtg aagaaataac tgctttatat atggctacaa agcaccattg
     2821 gtcatgtact caaagcatgg caaggtttta gagctagaaa tagcaagtta aaataaggct
     2881 agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttact agttacgggc
     2941 ccaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag
     3001 aagaccaaag ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat
     3061 tccattgccc agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct
     3121 acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg
     3181 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
     3241 cgtcttcaaa gcaagtggat tgatgtgaac atggtggagc acgacactct cgtctactcc
     3301 aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
     3361 gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg
     3421 acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc
     3481 gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     3541 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc
     3601 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
     3661 ggaagttcat ttcatttgga gaggacacgc tgaaatcacc agtctctctc tacaaatcta
     3721 tctctctcga ccgctagcat ggacaagaag tactccattg ggctcgatat cggcacaaac
     3781 agcgtcggct gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa attcaaagtt
     3841 ctgggcaata ccgatcgcca cagcataaag aagaacctca ttggcgccct cctgttcgac
     3901 tccggggaga cggccgaagc cacgcggctc aaaagaacag cacggcgcag atatacccgc
     3961 agaaagaatc ggatctgcta cctgcaggag atctttagta atgagatggc taaggtggat
     4021 gactctttct tccataggct ggaggagtcc tttttggtgg aggaggataa aaagcacgag
     4081 cgccacccaa tctttggcaa tatcgtggac gaggtggcgt accatgaaaa gtacccaacc
     4141 atatatcatc tgaggaagaa gcttgtagac agtactgata aggctgactt gcggttgatc
     4201 tatctcgcgc tggcgcatat gatcaaattt cggggacact tcctcatcga gggggacctg
     4261 aacccagaca acagcgatgt cgacaaactc tttatccaac tggttcagac ttacaatcag
     4321 cttttcgaag agaacccgat caacgcatcc ggagttgacg ccaaagcaat cctgagcgct
     4381 aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac agctccctgg ggagaagaag
     4441 aacggcctgt ttggtaatct tatcgccctg tcactcgggc tgacccccaa ctttaaatct
     4501 aacttcgacc tggccgaaga tgccaagctt caactgagca aagacaccta cgatgatgat
     4561 ctcgacaatc tgctggccca gatcggcgac cagtacgcag accttttttt ggcggcaaag
     4621 aacctgtcag acgccattct gctgagtgat attctgcgag tgaacacgga gatcaccaaa
     4681 gctccgctga gcgctagtat gatcaagcgc tatgatgagc accaccaaga cttgactttg
     4741 ctgaaggccc ttgtcagaca gcaactgcct gagaagtaca aggaaatttt cttcgatcag
     4801 tctaaaaatg gctacgccgg atacattgac ggcggagcaa gccaggagga attttacaaa
     4861 tttattaagc ccatcttgga aaaaatggac ggcaccgagg agctgctggt aaagcttaac
     4921 agagaagatc tgttgcgcaa acagcgcact ttcgacaatg gaagcatccc ccaccagatt
     4981 cacctgggcg aactgcacgc tatcctcagg cggcaagagg atttctaccc ctttttgaaa
     5041 gataacaggg aaaagattga gaaaatcctc acatttcgga taccctacta tgtaggcccc
     5101 ctcgcccggg gaaattccag attcgcgtgg atgactcgca aatcagaaga gaccatcact
     5161 ccctggaact tcgaggaagt cgtggataag ggggcctctg cccagtcctt catcgaaagg
     5221 atgactaact ttgataaaaa tctgcctaac gaaaaggtgc ttcctaaaca ctctctgctg
     5281 tacgagtact tcacagttta taacgagctg accaaggtca aatacgtcac agaagggatg
     5341 agaaagccag cattcctgtc tggagagcag aagaaagcta tcgtggacct cctcttcaag
     5401 acgaaccgga aagttaccgt gaaacagctc aaagaagact atttcaaaaa gattgaatgt
     5461 ttcgactctg ttgaaatcag cggagtggag gatcgcttca acgcatccct gggaacgtat
     5521 cacgatctcc tgaaaatcat taaagacaag gacttcctgg acaatgagga gaacgaggac
     5581 attcttgagg acattgtcct cacccttacg ttgtttgaag atagggagat gattgaagaa
     5641 cgcttgaaaa cttacgctca tctcttcgac gacaaagtca tgaaacagct caagaggcgc
     5701 cgatatacag gatgggggcg gctgtcaaga aaactgatca atggcatccg agacaagcag
     5761 agtggaaaga caatcctgga ttttcttaag tccgatggat ttgccaaccg gaacttcatg
     5821 cagttgatcc atgatgactc tctcaccttt aaggaggaca tccagaaagc acaagtttct
     5881 ggccaggggg acagtcttca cgagcacatc gctaatcttg caggtagccc agctatcaaa
     5941 aagggaatac tgcagaccgt taaggtcgtg gatgaactcg tcaaagtaat gggaaggcat
     6001 aagcccgaga atatcgttat cgagatggcc cgagagaacc aaactaccca gaagggacag
     6061 aagaacagta gggaaaggat gaagaggatt gaagagggta taaaagaact ggggtcccaa
     6121 atccttaagg aacacccagt tgaaaacacc cagcttcaga atgagaagct ctacctgtac
     6181 tacctgcaga acggcaggga catgtacgtg gatcaggaac tggacatcaa tcggctctcc
     6241 gactacgacg tggatcatat cgtgccccag tcttttctca aagatgattc tattgataat
     6301 aaagtgttga caagatccga taaaaataga gggaagagtg ataacgtccc ctcagaagaa
     6361 gttgtcaaga aaatgaaaaa ttattggcgg cagctgctga acgccaaact gatcacacaa
     6421 cggaagttcg ataatctgac taaggctgaa cgaggtggcc tgtctgagtt ggataaagcc
     6481 ggcttcatca aaaggcagct tgttgagaca cgccagatca ccaagcacgt ggcccaaatt
     6541 ctcgattcac gcatgaacac caagtacgat gaaaatgaca aactgattcg agaggtgaaa
     6601 gttattactc tgaagtctaa gctggtctca gatttcagaa aggactttca gttttataag
     6661 gtgagagaga tcaacaatta ccaccatgcg catgatgcct acctgaatgc agtggtaggc
     6721 actgcactta tcaaaaaata tcccaagctt gaatctgaat ttgtttacgg agactataaa
     6781 gtgtacgatg ttaggaaaat gatcgcaaag tctgagcagg aaataggcaa ggccaccgct
     6841 aagtacttct tttacagcaa tattatgaat tttttcaaga ccgagattac actggccaat
     6901 ggagagattc ggaagcgacc acttatcgaa acaaacggag aaacaggaga aatcgtgtgg
     6961 gacaagggta gggatttcgc gacagtccgg aaggtcctgt ccatgccgca ggtgaacatc
     7021 gttaaaaaga ccgaagtaca gaccggaggc ttctccaagg aaagtatcct cccgaaaagg
     7081 aacagcgaca agctgatcgc acgcaaaaaa gattgggacc ccaagaaata cggcggattc
     7141 gattctccta cagtcgctta cagtgtactg gttgtggcca aagtggagaa agggaagtct
     7201 aaaaaactca aaagcgtcaa ggaactgctg ggcatcacaa tcatggagcg atcaagcttc
     7261 gaaaaaaacc ccatcgactt tctcgaagcg aaaggatata aagaggtcaa aaaagacctc
     7321 atcattaagc ttcccaagta ctctctcttt gagcttgaaa acggccggaa acgaatgctc
     7381 gctagtgcgg gcgagctgca gaaaggtaac gagctggcac tgccctctaa atacgttaat
     7441 ttcttgtatc tggccagcca ctatgaaaag ctcaaagggt ctcccgaaga taatgagcag
     7501 aagcagctgt tcgtggaaca acacaaacac taccttgatg agatcatcga gcaaataagc
     7561 gagttctcca aaagagtgat cctcgccgac gctaacctcg ataaggtgct ttctgcttac
     7621 aataagcaca gggataagcc catcagggag caggcagaaa acattatcca cttgtttact
     7681 ctgaccaact tgggcgcgcc tgcagccttc aagtacttcg acaccaccat agacagaaag
     7741 cggtacacct ctacaaagga ggtcctggac gccacactga ttcatcagtc aattacgggg
     7801 ctctatgaaa caagaatcga cctctctcag ctcggtggag acggaacaag aaagagaaga
     7861 aaggtgtgac ggatcctcga atatgaagat gaagatgaaa tatttggtgt gtcaaataaa
     7921 aagcttgtgt gcttaagttt gtgttttttt cttggcttgt tgtgttatga atttgtggct
     7981 ttttctaata tcaaatgaat gtaagatctc attataatga ataaacaaat gtttctataa
     8041 tccattgtga atgttttgtt ggatctcttc tgcagcatat aactactgta tgtgctatgg
     8101 tatggactat ggaatatgat taaagataag gtttaaacta tcagtgtttg acaggatata
     8161 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     8221 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg agatccaaaa
     8281 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8341 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8401 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8461 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8521 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8581 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8641 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8701 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8761 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8821 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8881 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8941 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9001 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     9061 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     9121 ccgagcgcag cgagtcagtg agcgaggaag cggaagagga tctcagtaaa gcgctggctg
     9181 ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc accaggtcat
     9241 cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga
     9301 cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg cggaaggttt
     9361 ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg
     9421 tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca
     9481 gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg ccacggtcca
     9541 ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg tcggacgtga
     9601 agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg taataccggc
     9661 cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc
     9721 cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg tcatcgtcgg
     9781 cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg aaaatgacct
     9841 tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg gcagagcggg
     9901 ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa
     9961 gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcttgc ttggcctcgc
    10021 tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc atagttcctc
    10081 gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga cgacgcgaac
    10141 gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct
    10201 cgatcttggc cgtagcttgc tgggccatcg agccgacgga ctggaaggtt tcgcggggcg
    10261 cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga
    10321 tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct ccttgcggga
    10381 ttgccccgac tcacgccggg gcaatgtgcc cttattcctg atttgatccg cctggtgcct
    10441 tggtgtccag ataatccacc ttatcggcaa tgaagtcggt cccgtagacc gtctggccgt
    10501 ccttctcgta cttggtattc cgaatcttgc cctgcacgaa taccagcgac cccttgccca
    10561 aatacttgcc gtgggcctcg gcctgagagc caaaacactt gatgcggaag aagtcggtgc
    10621 gctcctgctt gtcgccagca tcgttgcgcc acatctaggt actaaaacaa ttcatccagt
    10681 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
    10741 tcgacatact gttcttcccc aatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
    10801 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
    10861 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
    10921 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggggt gtcttcttcc
    10981 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
    11041 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
    11101 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
    11161 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
    11221 aggtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
    11281 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
    11341 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
    11401 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
    11461 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
    11521 cgaactccaa ttcactgttc cttgcattct aaaactttaa ataccagaaa acagcttttt
    11581 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccac
    11641 aattatgggt gatgctgcca acgcgagagc gggccgggag ggttcgagaa gggggggcac
    11701 cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta
    11761 taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa aacgggcgga
    11821 aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc aataggtgcg
    11881 cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct catctgtcag
    11941 tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt gtccacatca
    12001 tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct ggccagctcc
    12061 acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg ggtgagtcgg
    12121 cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt tccgcgaggt
    12181 atccacaacg ccggcggctg cggtgtctcg cacacggctt cgacggcgtt tctggcgcgt
    12241 ttgcagggcc atagacggcc gccagcccag cggcgagggc aaccagcccg gtgagcgtct
    12301 ctagtggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
    12361 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgattt
//
