LOCUS       sequence-386364-       13327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Integratable Constitutive CD19CAR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13327)
  AUTHORS   Li HS, Israni DV, Gagnon KA, Gan KA, Raymond MH, Sander JD, Roybal 
            KT, Joung JK, Wong WW, Khalil AS
  TITLE     Multidimensional control of therapeutic human cell function with 
            synthetic gene circuits.
  JOURNAL   Science. 2022 Dec 16;378(6625):1227-1234. doi: 
            10.1126/science.ade0156. Epub 2022 Dec 15.
  PUBMED    36520914
REFERENCE   2  (bases 1 to 13327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.ade0156"; journalName: "Science"; date: 
            "2022-12-16- 16"; volume: "378"; issue: "6625"; pages: "1227-1234"
COMMENT     Sequence Label: pBW-507
FEATURES             Location/Qualifiers
     source          1..13327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2..18)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             108..341
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(366..384)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(367..384)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(674..693)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(907..926)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1026..1048
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1086..1104)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1172..1276
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1277..1345,1346..2137)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1495..1514)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2308..2896
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3137..3157)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3142..3471
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3322..3457
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      4274..4862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     intron          6308..6373
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6503..6523
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6948..7082
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6985..7004)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7039..7058
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7250..7883
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7930..8055
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8552..8785
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8970..9014
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9299..9416
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9569..9976
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     protein_bind    10010..10043
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      10083..10092
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10878..10907
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             11724..12434
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(11869..11887)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     12137..12156
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    12474..12507
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    complement(12610..12643)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(12644..12677)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     misc_feature    12737..13325
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12790..12810)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(13208..13219)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
       61 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
      121 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
      181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      301 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
      361 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
      421 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
      481 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
      541 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
      601 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      661 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      721 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      781 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      841 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
      901 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      961 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     1021 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     1081 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     1141 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1201 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1261 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1321 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1381 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1441 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1501 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1561 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1621 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1681 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1741 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1801 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1861 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1921 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1981 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2041 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2101 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2161 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2221 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2281 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2341 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2401 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2461 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2521 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2581 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2641 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2701 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2761 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2821 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2881 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2941 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3001 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3061 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     3121 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     3181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     3241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     3301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     3361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     3421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga
     3481 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
     3541 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
     3601 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     3661 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     3721 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     3781 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     3841 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     3901 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
     3961 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
     4021 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag
     4081 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     4141 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
     4201 tattctggag gctgcatcca tgacacaggc aaacctgagc gtcagacccc gtagaaaaga
     4261 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4321 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4381 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     4441 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4501 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4561 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4621 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4681 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4741 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4801 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4861 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4921 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     4981 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5041 aagagcgccc aatacgcmaa ccrsmtstcc smgckcgwtr kyykaywcrw ywmtsywtmy
     5101 cwrttctgcc ccgaattaca cttaaaacga ctcaacagct taacgttggc ttgccacgca
     5161 ttacttgact gtaaaactct cactcttacc gaacttggcc gtaaccgttt gagaatacca
     5221 ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg
     5281 gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt
     5341 taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg
     5401 gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg
     5461 aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac
     5521 tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg
     5581 agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc
     5641 tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg
     5701 tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc
     5761 cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct
     5821 ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc
     5881 gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg
     5941 agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc
     6001 tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac
     6061 tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc
     6121 ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac
     6181 gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt
     6241 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
     6301 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
     6361 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
     6421 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
     6481 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
     6541 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
     6601 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
     6661 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct
     6721 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt
     6781 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag
     6841 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     6901 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     6961 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7021 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     7081 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
     7141 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
     7201 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
     7261 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     7321 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     7381 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     7441 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     7501 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     7561 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     7621 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     7681 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7741 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7801 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7861 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     7921 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     7981 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     8041 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     8101 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     8161 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     8221 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     8281 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     8341 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
     8401 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     8461 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     8521 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     8581 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     8641 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     8701 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     8761 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     8821 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     8881 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     8941 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     9001 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     9061 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     9121 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     9181 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     9241 ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc atccacaatt
     9301 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     9361 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     9421 tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc gaattcctgc
     9481 agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc
     9541 cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat ggaaaaatac
     9601 caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc taacgttggg
     9661 ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag aacagatggt
     9721 caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat atggcccaac
     9781 cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga cctgaaatga
     9841 ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc gcgcgcttct
     9901 gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag tcctccgaca
     9961 gactgagtcg cccggggggg atctggagct ctcgagaatt ctcacgcgcg aagttcctat
    10021 tctctagaaa gtataggaac ttcctttacc tactacggtc gaccttacct tcgggatccg
    10081 ccgccaccat ggccttacca gtgaccgcct tgctcctgcc gctggccttg ctgctccacg
    10141 ccgccaggcc ggacatccag atgacacaga ctacatcctc cctgtctgcc tctctgggag
    10201 acagagtcac catcagttgc agggcaagtc aggacattag taaatattta aattggtatc
    10261 agcagaaacc agatggaact gttaaactcc tgatctacca tacatcaaga ttacactcag
    10321 gagtcccatc aaggttcagt ggcagtgggt ctggaacaga ttattctctc accattagca
    10381 acctggagca agaagatatt gccacttact tttgccaaca gggtaatacg cttccgtaca
    10441 cgttcggagg cggcaccaag ctggagatca caggtggcgg tggctcgggc ggtggtgggt
    10501 cgggtggcgg cggatctgag gtgaaactgc aggagtcagg acctggcctg gtggcgccct
    10561 cacagagcct gtccgtcaca tgcactgtct caggggtctc attacccgac tatggtgtaa
    10621 gctggattcg ccagcctcca cgaaagggtc tggagtggct gggagtaata tggggtagtg
    10681 aaaccacata ctataattca gctctcaaat ccagactgac catcatcaag gacaactcca
    10741 agagccaagt tttcttaaaa atgaacagtc tgcaaactga tgacacagcc atttactact
    10801 gtgccaaaca ttattactac ggtggtagct atgctatgga ctactggggc caaggaacct
    10861 cagtcaccgt ctcctcagaa caaaaactca tctcagaaga agatctgaat ggggccgcaa
    10921 ccacgacgcc agcgccgcga ccaccaacac cggcgcccac catcgcgttg cagcccctgt
    10981 ccctgcgccc agaggcgtgc cggccagcgg cggggggcgc agtgcacacg agggggctgg
    11041 acttcgcctg tgatttttgg gtgctggtgg tggttggtgg agtcctggct tgctatagct
    11101 tgctagtaac agtggccttt attattttct gggtgaggag taagaggagc aggctcctgc
    11161 acagtgacta catgaacatg actccccgcc gccccgggcc cacccgcaag cattaccagc
    11221 cctatgcccc accacgcgac ttcgcagcct atcgctccaa acggggcaga aagaaactcc
    11281 tgtatatatt caaacaacca tttatgagac cagtacaaac tactcaagag gaagatggct
    11341 gtagctgccg atttccagaa gaagaagaag gaggatgtga actgagagtg aagttcagca
    11401 ggagcgcaga cgcccccgcg tacaagcagg gccagaacca gctctataac gagctcaatc
    11461 taggacgaag agaggagtac gatgttttgg acaagagacg tggccgggac cctgagatgg
    11521 ggggaaagcc gagaaggaag aaccctcagg aaggcctgta caatgaactg cagaaagata
    11581 agatggcgga ggcctacagt gagattggga tgaaaggcga gcgccggagg ggcaaggggc
    11641 acgatggcct ttaccagggt ctcagtacag ccaccaagga cacctacgac gcccttcaca
    11701 tgcaggccct gccccctcgc ggcatggtga gcaagggcga ggaggataac atggccatca
    11761 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
    11821 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
    11881 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
    11941 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
    12001 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
    12061 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
    12121 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
    12181 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
    12241 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
    12301 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
    12361 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
    12421 agctgtacaa gtaagtttaa actatattct aaggcgttac cctgttcagt ttggaagttc
    12481 ctattcttca aatagtatag gaacttccgc actcggagct aacttgtcgg gacgaggact
    12541 agtacggaca gcttggtatc ctaagcgtag tcgacgcgta ctaatagcaa caacgatcgg
    12601 ccgcaccttg aagttcctat actttctaga gaataggaac ttcgaagttc ctatactatt
    12661 tgaagaatag gaacttcgcg gccgcgactc tagagtcgac ctgcaggcat gcaagcttga
    12721 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
    12781 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
    12841 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
    12901 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
    12961 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
    13021 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
    13081 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
    13141 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
    13201 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
    13261 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
    13321 cccgcat
//
