LOCUS       sequence-386375-       11404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express 3xHA-tagged human KCNJ6 (Girk2), co-expressed with mCherry, 
            from UbiC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11404)
  TITLE     Hart Lab Plasmids
REFERENCE   2  (bases 1 to 11404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FUGW-3xHA-KCNJ6-mCherry
FEATURES             Location/Qualifiers
     source          1..11404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1270..1296
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1303..1329
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1336..1362
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2629..2682
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2695..2715
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2716..3426
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGREFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2861..2879)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3129..3148
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3323..3346
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    3475..4063
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3528..3548)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3946..3957)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4066..4082)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4067..4083)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4588..4768
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4794..4811)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4800..5024
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5070..5498
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5157..5176)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5367..5388
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5507..5527)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5512..5841
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5692..5827
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5754..5773
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5889..5936
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5955..6329
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6459..6580
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6496..6515)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6550..6569
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6629..6645)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6629..6645)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6642..6664)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6653..6669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6677..6683,6684..6701,6702..6707))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6722..6743
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6860..6877)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7031..7619)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7111..7130)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7790..8581,8582..8650))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8413..8432
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8651..8755)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8830..8849)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9021..9400
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9402..9600
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9555..9575
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9618..9798
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9845..9970
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10463..10696
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10881..10925
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11223..11340
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactgaccg atccagcctc cgcggccccg
     1261 gccaccatgt acccatacga tgttccagat tacgctggat cttacccata cgatgttcca
     1321 gattacgctg gatcttaccc atacgatgtt ccagattacg ctgccaagct gacagaatcc
     1381 atgactaacg tcctggaggg cgactccatg gatcaggacg tcgaaagccc agtggccatt
     1441 caccagccaa agttgcctaa gcaggccagg gatgacctgc caagacacat cagccgagat
     1501 cggaccaaaa ggaaaatcca gaggtacgtg aggaaagacg gaaagtgcaa tgttcatcac
     1561 ggcaacgtga gggagaccta tcgctacctg accgatatct tcaccacatt agtggacctg
     1621 aagtggagat tcaacctatt gatttttgtc atggtttaca cagtgacctg gctctttttt
     1681 ggaatgatct ggtggttgat cgcatacata cggggagaca tggaccacat agaggacccc
     1741 tcctggactc cttgtgttac caacctcaac gggttcgtct ctgctttttt attctcaata
     1801 gagacagaaa ccaccattgg ttatggctac cgggtcatca cagataaatg cccagaggga
     1861 attattcttc tcttaatcca atctgtgttg gggtccattg tcaatgcatt catggtggga
     1921 tgcatgtttg taaaaatctc tcaacccaag aagagggcag agaccctggt cttttccacc
     1981 catgcagtga tctccatgcg ggatgggaaa ctgtgcctga tgttccgggt aggggacctt
     2041 aggaattccc acattgtgga ggcttccatc agagccaagt tgatcaaatc caaacagacc
     2101 tcggaggggg agttcatccc gttgaaccag acggatatca acgtagggta ttacacgggg
     2161 gatgaccgtc tgtttctggt gtcaccgctg atcattagcc atgaaattaa ccaacagagt
     2221 cctttctggg agatctccaa agcccagctg cccaaagagg aactggaaat tgtggtcatc
     2281 ctagaaggaa tggtggaagc cacagggatg acatgccaag ctcgaagctc ctacatcacc
     2341 agtgagatcc tgtggggtta ccggttcaca cctgtcctga ccctggagga tgggttctac
     2401 gaagttgact acaacagctt ccatgagacc tatgagacca gcaccccatc ccttagtgcc
     2461 aaagagctgg ccgagttagc cagcagggca gagctgcccc tgagttggtc tgtatccagc
     2521 aaactcaacc aacatgcaga actggagact gaagaggaag aaaagaacct cgaagagcaa
     2581 acagaaagaa atggtgatgt ggcaaacctg gagaatgaat ccaaagttga gggcagggga
     2641 agtcttctaa catgcgggga cgtggaggaa aatcccggcc ctctagagat ggctccaaag
     2701 aagaagcgta aggtaatggt gagcaagggc gaggaggaca acatggccat catcaaggag
     2761 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccgcgagtt cgagatcgag
     2821 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     2881 ggcggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     2941 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     3001 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     3061 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     3121 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     3181 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     3241 ggccactacg acgccgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     3301 ggcgcctaca acgtcaacat caagctggac atcacctccc acaacgagga ctacaccatc
     3361 gtggaacagt acgagcgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     3421 aagtaaatta agctagctaa ttcgatatca aaattcgata tcaagcttat cgataatcaa
     3481 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3541 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3601 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3661 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3721 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3781 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3841 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3901 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3961 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4021 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     4081 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     4141 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     4201 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4261 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     4321 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     4381 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     4441 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     4501 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     4561 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     4621 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     4681 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     4741 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
     4801 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4861 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4921 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4981 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     5041 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     5101 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5161 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5221 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5281 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5341 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5401 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5461 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     5521 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5581 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5641 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5701 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5761 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     5821 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     5881 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     5941 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     6001 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     6061 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     6121 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     6181 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     6241 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     6301 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     6361 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     6421 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     6481 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6541 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     6601 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6661 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6721 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6781 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     6841 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     6901 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     6961 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7021 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7081 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7141 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7201 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7261 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7321 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7381 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7441 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     7501 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7561 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7621 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7681 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7741 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7801 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7861 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7921 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7981 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8041 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8101 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8161 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8221 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8281 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8341 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8401 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8461 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8521 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8581 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8641 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8701 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8761 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     8821 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     8881 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     8941 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     9001 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     9061 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     9121 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     9181 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     9241 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     9301 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     9361 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     9421 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     9481 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     9541 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     9601 cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9661 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     9721 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     9781 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     9841 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     9901 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     9961 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
    10021 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
    10081 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
    10141 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
    10201 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
    10261 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
    10321 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
    10381 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
    10441 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
    10501 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
    10561 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
    10621 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
    10681 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
    10741 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
    10801 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
    10861 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
    10921 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
    10981 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
    11041 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
    11101 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
    11161 tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac
    11221 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
    11281 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
    11341 cgggtttatt acagggacag cagagatcca gtttggttaa ttaacccgtg tcggctccag
    11401 atct
//
