LOCUS       sequence-386379-        8634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CRKL open, V5 tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Cheung HW, Du J, Boehm JS, He F, Weir BA, Wang X, Butaney M, Sequist
            LV, Luo B, Engelman JA, Root DE, Meyerson M, Golub TR, Janne PA, 
            Hahn WC
  TITLE     Amplification of CRKL induces transformation and epidermal growth 
            factor receptor inhibitor resistance in human non-small cell lung 
            cancers.
  JOURNAL   Cancer Discov. 2011 Dec;1(7):608-25. doi: 
            10.1158/2159-8290.CD-11-0046. Epub 2011 Oct 17.
  PUBMED    22586683
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-11-0046"; journalName: "Cancer Discov"; date: 
            "2011-12"; volume: "1"; issue: "7"; pages: "608-25"
COMMENT     Sequence Label: plx304_CRKL-open-V5
FEATURES             Location/Qualifiers
     source          1..8634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     524..544
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    596..620
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1533..1557)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1559..1600
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1642..2230
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1695..1715)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2113..2124)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2302..2535
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2613..2734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2650..2669)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2704..2723
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2774..2909
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2929..2948)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2930..2948)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2958..2975)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2958..2974)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2967..2989)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3116..3571
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3203..3222)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3413..3434
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3597..3701
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3702..3770,3771..4562)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3920..3939)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4733..5321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5222..5241
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5475..5492
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5609..5630
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5645..5650,5651..5668,5669..5675)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5683..5699
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5688..5710
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5707..5723
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5707..5723
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5742..5762
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5744..5762
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5790..6016
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6017..6197
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6244..6369
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6862..7095
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7280..7324
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7499..7999
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7918..7936
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8011..8406
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8466..8583
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcccggggtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      541 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag
      601 tttgtacaaa aaagttggca ccatgtcctc cgccaggttc gactcctcgg accgctccgc
      661 ctggtatatg gggccggtgt ctcgccagga ggcgcagacc cggctccagg gccagcgcca
      721 cggtatgttc ctcgtccgcg attcttccac ctgccctggg gactatgtgc tgtcggtgtc
      781 cgagaactcg cgggtctccc actacatcat caactcgctg cccaaccgcc gttttaagat
      841 cggggaccag gaatttgacc atttgccggc cctgctggag ttttacaaga tccactacct
      901 ggacaccacc accctcatcg agcctgcgcc caggtatcca agcccaccaa tgggatctgt
      961 ctcagcaccc aacctgccta cagcagaaga taacctggaa tatgtacgga ctctgtatga
     1021 ttttcctggg aatgatgccg aagacctgcc ctttaaaaag ggtgagatcc tagtgataat
     1081 agagaagcct gaagaacagt ggtggagtgc ccggaacaag gatggccggg ttgggatgat
     1141 tcctgtccct tatgtcgaaa agcttgtgag atcctcacca cacggaaagc atggaaatag
     1201 gaattccaac agttatggga tcccagaacc tgctcatgca tacgctcaac ctcagaccac
     1261 aactcctcta cctgcagttt ccggttctcc tggggcagca atcacccctt tgccatccac
     1321 acagaatgga cctgtctttg cgaaagcaat ccagaaaaga gtaccctgtg cttatgacaa
     1381 gactgccttg gcattagagg ttggtgacat cgtgaaagtc acaaggatga atataaatgg
     1441 ccagtgggaa ggcgaagtga acgggcgcaa agggcttttc ccctttacgc acgtcaaaat
     1501 ctttgaccct caaaacccag atgaaaacga gttgccaact ttcttgtaca aagtggttgg
     1561 taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag ctagcgctaa
     1621 ccggtggcgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     1681 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     1741 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     1801 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     1861 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     1921 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     1981 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     2041 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     2101 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     2161 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     2221 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     2281 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     2341 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     2401 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     2461 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     2521 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     2581 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     2641 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2701 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     2761 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     2821 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     2881 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     2941 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     3001 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     3061 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     3121 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3181 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3241 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     3301 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     3361 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     3421 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     3481 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     3541 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     3601 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3661 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     3721 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     3781 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     3841 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     3901 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     3961 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4021 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4081 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4141 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4201 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4261 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4321 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4381 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4441 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4501 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4561 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4621 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4681 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4741 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4801 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4861 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4921 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     4981 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5041 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5101 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5161 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5221 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5281 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5341 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5401 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5461 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     5521 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     5581 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     5641 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     5701 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     5761 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     5821 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     5881 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     5941 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     6001 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6061 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6121 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6181 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     6241 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     6301 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     6361 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     6421 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     6481 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     6541 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     6601 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     6661 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     6721 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     6781 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     6841 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     6901 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     6961 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     7021 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     7081 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     7141 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     7201 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     7261 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     7321 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     7381 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     7441 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggaccctt
     7501 gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc
     7561 cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt
     7621 cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct
     7681 aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg
     7741 tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg
     7801 atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg
     7861 ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg
     7921 ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat
     7981 caccgacctc tctccccagg gggtaccacc atggccaagc ctttgtctca agaagaatcc
     8041 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
     8101 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
     8161 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
     8221 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
     8281 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
     8341 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
     8401 gagggcctgc agctgcagta gtaagaattc tagatcttga gacaaatggc agtattcatc
     8461 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     8521 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     8581 tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga gggg
//
