LOCUS       sequence-386421-        8644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CRKL Y207F, V5 tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8644)
  AUTHORS   Cheung HW, Du J, Boehm JS, He F, Weir BA, Wang X, Butaney M, Sequist
            LV, Luo B, Engelman JA, Root DE, Meyerson M, Golub TR, Janne PA, 
            Hahn WC
  TITLE     Amplification of CRKL induces transformation and epidermal growth 
            factor receptor inhibitor resistance in human non-small cell lung 
            cancers.
  JOURNAL   Cancer Discov. 2011 Dec;1(7):608-25. doi: 
            10.1158/2159-8290.CD-11-0046. Epub 2011 Oct 17.
  PUBMED    22586683
REFERENCE   2  (bases 1 to 8644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-11-0046"; journalName: "Cancer Discov"; date: 
            "2011-12"; volume: "1"; issue: "7"; pages: "608-25"
COMMENT     Sequence Label: pLenti6.3_CRKL-Y207F-V5
FEATURES             Location/Qualifiers
     source          1..8644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1692
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1715..2018
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2019..2222
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2173..2193
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2219..2243
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2339..2363
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3276..3300)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3353..3394
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3418..4006
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3471..3491)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3889..3900)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4021..4337
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4188..4323
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4385..4432
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4451..4849
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4936..5169
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5241..5362
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5278..5297)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5332..5351
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5402..5537
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5557..5576)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5558..5576)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5586..5603)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5586..5602)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5595..5617)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5744..6199
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5831..5850)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6041..6062
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6225..6329
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6330..6398,6399..7190)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6548..6567)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7361..7949
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7850..7869
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8103..8120
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8237..8258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8273..8278,8279..8296,8297..8303)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8311..8327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8316..8338
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8335..8351
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8335..8351
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8370..8390
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8372..8390
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8418..8644
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 attcaaaatt ttatcgataa gcttgggagt tccgcgttac ataacttacg gtaaatggcc
     1741 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     1801 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     1861 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     1921 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     1981 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     2041 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     2101 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     2161 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     2221 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2281 gaagacaccg actctagagg atccactagt ccagtgtggt ggaattctgc agatatcaac
     2341 aagtttgtac aaaaaagttg gcaccatgtc ctccgccagg ttcgactcct cggaccgctc
     2401 cgcctggtat atggggccgg tgtctcgcca ggaggcgcag acccggctcc agggccagcg
     2461 ccacggtatg ttcctcgtcc gcgattcttc cacctgccct ggggactatg tgctgtcggt
     2521 gtccgagaac tcgcgggtct cccactacat catcaactcg ctgcccaacc gccgttttaa
     2581 gatcggggac caggaatttg accatttgcc ggccctgctg gagttttaca agatccacta
     2641 cctggacacc accaccctca tcgagcctgc gcccaggtat ccaagcccac caatgggatc
     2701 tgtctcagca cccaacctgc ctacagcaga agataacctg gaatatgtac ggactctgta
     2761 tgattttcct gggaatgatg ccgaagacct gccctttaaa aagggtgaga tcctagtgat
     2821 aatagagaag cctgaagaac agtggtggag tgcccggaac aaggatggcc gggttgggat
     2881 gattcctgtc ccttatgtcg aaaagcttgt gagatcctca ccacacggaa agcatggaaa
     2941 taggaattcc aacagttatg ggatcccaga acctgctcat gcattcgctc aacctcagac
     3001 cacaactcct ctacctgcag tttccggttc tcctggggca gcaatcaccc ctttgccatc
     3061 cacacagaat ggacctgtct ttgcgaaagc aatccagaaa agagtaccct gtgcttatga
     3121 caagactgcc ttggcattag aggttggtga catcgtgaaa gtcacaagga tgaatataaa
     3181 tggccagtgg gaaggcgaag tgaacgggcg caaagggctt ttccccttta cgcacgtcaa
     3241 aatctttgac cctcaaaacc cagatgaaaa cgagttgcca actttcttgt acaaagtggt
     3301 tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc
     3361 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gatcgacaat
     3421 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3481 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3541 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3601 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3661 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3721 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3781 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     3841 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3901 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3961 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg cgatggtacc
     4021 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4081 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4141 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4201 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4261 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4321 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     4381 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     4441 gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     4501 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
     4561 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     4621 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     4681 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     4741 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     4801 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacaattcga
     4861 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4921 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     4981 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5041 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5101 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5161 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     5221 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     5281 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5341 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     5401 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5461 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5521 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     5581 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     5641 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     5701 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     5761 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5821 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5881 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5941 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6001 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6061 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6121 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     6181 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6241 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6301 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6361 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6421 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6481 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6541 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6601 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6661 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6721 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6781 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6841 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6901 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6961 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7021 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7081 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7141 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7201 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7261 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7321 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7381 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7441 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7501 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7561 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7621 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7681 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7741 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7801 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7861 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7921 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7981 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8041 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8101 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8161 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     8221 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     8281 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     8341 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     8401 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     8461 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     8521 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     8581 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     8641 aaac
//
