LOCUS       sequence-386425-        8876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  single-particle tracking for mTBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Dong P, Zhang S, Xie L, Wang L, Lemire AL, Lander AD, Chang HY, Liu 
            ZJ
  TITLE     Spatial organization of the 3D genome encodes gene co-expression 
            programs in single cells
  JOURNAL   bioRxiv 2022.10.26.513917
REFERENCE   2  (bases 1 to 8876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.10.26.513917"
COMMENT     Sequence Label: piggybac-EF1-halo-mTBP
FEATURES             Location/Qualifiers
     source          1..8876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        135..346
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             359..627
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             650..670
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             686..1570
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     regulatory      2592..2601
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2606..3178
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2773..2790)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3000..3019
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3187..3981
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3241..3260)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3851..3870
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4041..4061)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    4103..4224
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4140..4159)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4194..4213
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4987..5021
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5512..5528)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5512..5528)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5525..5547)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5536..5552
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5560..5566,5567..5584,5585..5590))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5605..5626
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5743..5760)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5914..6502)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5994..6013)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6673..7464,7465..7533))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7296..7315
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7534..7638)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7664..8119)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7801..7822)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8013..8032
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8246..8268
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8260..8277
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8261..8277
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8407..8469
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ggcctccacg gccaattcca tatgccaagg atctgcgatc gctccggtgc ccgtcagtgg
       61 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattcgctc
      121 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
      181 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
      241 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
      301 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg
      361 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt
      421 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt
      481 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag
      541 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc
      601 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg acgcgtatgg aggatctgta
      661 ctttcagagc gataacgatg gatccgaaat cggtactggc tttccattcg acccccatta
      721 tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt ggtccgcgcg atggcacccc
      781 tgtgctgttc ctgcacggta acccgacctc ctcctacgtg tggcgcaaca tcatcccgca
      841 tgttgcaccg acccatcgct gcattgctcc agacctgatc ggtatgggca aatccgacaa
      901 accagacctg ggttatttct tcgacgacca cgtccgcttc atggatgcct tcatcgaagc
      961 cctgggtctg gaagaggtcg tcctggtcat tcacgactgg ggctccgctc tgggtttcca
     1021 ctgggccaag cgcaatccag agcgcgtcaa aggtattgca tttatggagt tcatccgccc
     1081 tatcccgacc tgggacgaat ggccagaatt tgcccgcgag accttccagg ccttccgcac
     1141 caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt tttatcgagg gtacgctgcc
     1201 gatgggtgtc gtccgcccgc tgactgaagt cgagatggac cattaccgcg agccgttcct
     1261 gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac gagctgccaa tcgccggtga
     1321 gccagcgaac atcgtcgcgc tggtcgaaga atacatggac tggctgcacc agtcccctgt
     1381 cccgaagctg ctgttctggg gcaccccagg cgttctgatc ccaccggccg aagccgctcg
     1441 cctggccaaa agcctgccta actgcaaggc tgtggacatc ggcccgggtc tgaatctgct
     1501 gcaagaagac aacccggacc tgatcggcag cgagatcgcg cgctggctgt ctactctgga
     1561 gatttccggt gaattcggct tctcgagggg tggcggcgga tctggcggtg gtgggtccgg
     1621 aggaggcggt tccatggacc agaacaacag ccttccacct tatgctcagg gcttggcctc
     1681 cccacagggc gccatgactc ctggaattcc catctttagt ccaatgatgc cttacggcac
     1741 aggacttact ccacagccta ttcagaacac caacagtctc tctattttgg aagagcaaca
     1801 aagacagcag cagcaacagc aacagcagca gcaacaacag caggcagtag caactgcagc
     1861 agcctcagta cagcaatcaa catctcagca acccacacag ggtgcctcag gccagacccc
     1921 acaactcttc cattctcaaa ctctgaccac tgcaccgttg ccaggcacca cccccttgta
     1981 cccttcacca atgactccta tgacccctat cactcctgcc acaccagctt ctgagagctc
     2041 tggaattgta ccgcagcttc aaaatattgt atctaccgtg aatcttggct gtaaacttga
     2101 cctaaagacc attgcacttc gtgcaagaaa tgctgaatat aatcccaagc gatttgctgc
     2161 agtcatcatg agaataagag agccacggac aactgcgttg attttcagtt ctggaaaaat
     2221 ggtgtgcaca ggagccaaga gtgaagaaca atccagacta gcagcaagaa aatatgctag
     2281 agttgtgcag aagttgggct tcccagctaa gttcttagac ttcaagatcc agaacatggt
     2341 ggggagctgt gatgtgaagt tccctataag gctggaaggc cttgtgctga cccaccagca
     2401 gttcagtagc tatgagccag aattatttcc tggattaatc tacagaatga tcaaacccag
     2461 aatcgttctc cttatttttg tttctggaaa agttgtatta acaggtgcta aagttagagc
     2521 agagatttat gaagcatttg aaaacatcta ccccatctta aagggattca ggaagaccac
     2581 atagttgtct gccatggcgg ccgcgcccct ctccctcccc cccccctaac gttactggcc
     2641 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     2701 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     2761 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     2821 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     2881 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     2941 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     3001 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     3061 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     3121 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc
     3181 tccggaatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     3241 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     3301 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     3361 gaactgcagg acgaggcagc gcggctatcg tggctggccg cgacgggcgt tccttgcgca
     3421 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     3481 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     3541 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     3601 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     3661 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     3721 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     3781 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     3841 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     3901 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     3961 cttcttgacg agttcttctg agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     4021 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     4081 cctttgtatc atgcgttaac taaacttgtt tattgcagct tataatggtt acaaataaag
     4141 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4201 gtccaaactc atcaatgtat cttatcatgt ctggaattga ctcaaatgat gtcaattagt
     4261 ctatcagaag ctcatctggt ctcccttccg ggggacaaga catccctgtt taatatttaa
     4321 acagcagtgt tcccaaactg ggttcttata tcccttgctc tggtcaacca ggttgcaggg
     4381 tttcctgtcc tcacaggaac gaagtcccta aagaaacagt ggcagccagg tttagccccg
     4441 gaattgactg gattcctttt ttagggccca ttggtatggc tttttccccg tatcccccca
     4501 ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc
     4561 ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg
     4621 accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac
     4681 atccctgggg gctttggggg ggggctgtcc ctgatatcta taacaagaaa atatatatat
     4741 aataagttat cacgtaagta gaacatgaaa taacaatata attatcgtat gagttaaatc
     4801 ttaaaagtca cgtaaaagat aatcatgcgt cattttgact cacgcggtcg ttatagttca
     4861 aaatcagtga cacttaccgc attgacaagc acgcctcacg ggagctccaa gcggcgactg
     4921 agatgtccta aatgcacagc gacggattcg cgctatttag aaagagagag caatatttca
     4981 agaatgcatg cgtcaatttt acgcagacta tctttctagg gttaatctag ctgcatcagg
     5041 atcatatcgt cgggtctttt ttccggctca gtcatcgccc aagctggcgc tatctgggca
     5101 tcggggagga agaagcccgt gccttttccc gcgaggttga agcggcatgg aaagagtttg
     5161 ccgaggatga ctgctgctgc attgacgttg agcgaaaacg cacgtttacc atgatgattc
     5221 gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc acctgggata
     5281 ccagttcgtc gcggcttttc cggacacagt tccggatggt cagcccgaag cgcatcagca
     5341 acccgaacaa taccggcgac agccggaact gccgtgccgg tgtgcagatt aatgacagcg
     5401 gtgcggcgct gggatattac gtcagcgagg acgggtatcc tggctggatg ccgcagaaat
     5461 ggacatggat accccgtgag ttacccggcg ggcgcgcttg gcgtaatcat ggtcatagct
     5521 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     5581 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     5641 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     5701 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     5761 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5821 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5881 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5941 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6001 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6061 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6121 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6181 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6241 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6301 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6361 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6421 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6481 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6541 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6601 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6661 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6721 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6781 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6841 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6901 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6961 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7021 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7081 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7141 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7201 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7261 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7321 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7381 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7441 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7501 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7561 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7621 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     7681 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     7741 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     7801 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     7861 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     7921 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     7981 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     8041 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     8101 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     8161 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     8221 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     8281 cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg ggcagactcg cggtgcaaat
     8341 gtgttttaca gcgtgatgga gcagatgaag atgctcgaca cgctgcagaa cacgcagcta
     8401 gattaaccct agaaagataa tcatattgtg acgtacgtta aagataatca tgtgtaaaat
     8461 tgacgcatgt gttttatcgg tctgtatatc gaggtttatt tattaatttg aatagatatt
     8521 aagttttatt atatttacac ttacatacta ataataaatt caacaaacaa tttatttatg
     8581 tttatttatt tattaaaaaa aacaaaaact caaaatttct tctataaagt aacaaaactt
     8641 ttatgaggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
     8701 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
     8761 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
     8821 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaa
//
