LOCUS       sequence-386450-        4347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC23A1_STOP
FEATURES             Location/Qualifiers
     source          1..4347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1904..2003)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2020..2039)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2021..2039)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2044..2060)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2044..2060)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2162..2181)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2173..2982
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2240..2259)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3129..3717
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3618..3637
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3871..3888
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4047..4074)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4166..4252)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4301..4323
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4315..4332
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4316..4332
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgag ggcacaggag
      121 gacctggagg gaaggaccca gcacgagacc acacgcgatc caagcacacc actgcccacc
      181 gagcccaagt tcgacatgct gtacaagatc gaggatgtgc ccccttggta tctgtgcatc
      241 ctgctgggct tccagcacta cctgacatgt ttttctggca ccatcgcagt gccctttctg
      301 ctggcagagg ccctgtgcgt gggacacgac cagcacatgg tgagccagct gatcggcaca
      361 atcttcacct gcgtgggcat caccacactg atccagacca cagtgggcat ccggctgccc
      421 ctgtttcagg cctccgcctt cgcctttctg gtgcctgcca aggccatcct ggccctggag
      481 agatggaagt gcccacccga ggaggagatc tacggcaatt ggtctctgcc tctgaacacc
      541 agccacatct ggcacccaag gatcagggag gtgcagggag ccatcatggt gagctccgtg
      601 gtggaggtgg tcatcggact gctgggactg cctggcgccc tgctgaatta tatcggccct
      661 ctgaccgtga caccaaccgt gagcctgatc ggactgtccg tgttccaggc agcaggcgat
      721 agggcaggat cccactgggg aatctccgcc tgctctatcc tgctgatcat cctgttctct
      781 cagtacctgc gcaacctgac atttctgctg cccgtgtaca gatggggcaa gggactgacc
      841 ctgctgagaa tccagatctt caagatgttt ccaatcatgc tggccatcat gaccgtgtgg
      901 ctgctgtgct acgtgctgac actgaccgac gtgctgccaa cagatcccaa ggcctatgga
      961 tttcaggcaa ggaccgacgc aagaggcgat atcatggcca tcgccccatg gatcaggatc
     1021 ccttacccat gccagtgggg actgccaaca gtgaccgcag cagccgtgct gggaatgttc
     1081 agcgccaccc tggcaggcat catcgagtcc atcggcgact actatgcctg tgcaaggctg
     1141 gcaggagcac ctccaccacc tgtgcacgcc atcaatcgcg gcatctttac agagggcatc
     1201 tgctgtatca tcgcaggact gctgggaaca ggaaatggaa gcacctctag ctcccccaac
     1261 atcggcgtgc tgggcatcac caaagtggga tccaggagag tggtgcagta cggagcagca
     1321 atcatgctgg tgctgggaac aatcggcaag ttcaccgccc tgttttctag cctgcccgac
     1381 cctatcctgg gcggcatgtt ctgcacactg tttggcatga tcaccgccgt gggcctgtcc
     1441 aacctgcagt tcgtggatat gaactcctct cgcaatctgt tcgtgctggg cttttctatg
     1501 ttctttggcc tgacactgcc caactatctg gagagcaatc ctggcgccat caacaccggc
     1561 atcctggagg tggaccagat cctgatcgtg ctgctgacca cagagatgtt cgtgggcggc
     1621 tgtctggcct ttatcctgga taatacagtg cctggatctc cagaggagag gggactgatc
     1681 cagtggaagg caggagcaca cgcaaactcc gacatgagct cctctctgaa gtcttacgat
     1741 ttccccatcg gcatgggcat cgtgaagaga atcacctttc tgaagtatat ccccatctgc
     1801 cccgtgttca agggctttag ctcctctagc aaggaccaga tcgccatccc agaggataca
     1861 cccgagaata cagagaccgc cagcgtgtgc accaaggtgt aagacccagc tttcttgtac
     1921 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     1981 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac
     2041 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     2101 gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat
     2161 acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac
     2221 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     2281 acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     2341 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     2401 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     2461 actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     2521 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     2581 tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac
     2641 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     2701 tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     2761 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     2821 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     2881 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     2941 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     3001 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
     3061 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3121 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3181 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3241 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3301 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3361 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3421 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3481 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3541 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3601 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3661 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3721 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3781 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3841 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3901 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     3961 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
     4021 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     4081 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     4141 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     4201 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
     4261 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa
     4321 acgacggcca gtcttaagct cgggccc
//
