LOCUS       sequence-386463-        4355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 pGATA23 promoter with GG0 and GG1 golden gate linkers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Guiziou S, Maranas CJ, Chu JC, Nemhauser JL
  TITLE     An integrase toolbox to record gene-expression during plant 
            development.
  JOURNAL   Nat Commun. 2023 Apr 3;14(1):1844. doi: 10.1038/s41467-023-37607-5.
  PUBMED    37012288
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-3"; volume: "14(1):1844. doi"; pages: " 
            10.1038/s41467-023-37607-5"
COMMENT     Sequence Label: pNL2080 pGATA23 GG0-GG1
FEATURES             Location/Qualifiers
     source          1..4355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..312)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(112..131)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     435..457
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     449..466
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     450..466
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     473..492
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        473..491
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1514..1530)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1514..1530)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1527..1549)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1538..1554
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1562..1568,1569..1586,1587..1592))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1607..1628
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1745..1762)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1916..2504)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1996..2015)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2642..3016)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      3013..3022
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3222..4016)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3222..4016)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3333..3352)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3943..3962
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg
       61 gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg ccatatcggt
      121 ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt cacgggagac
      181 tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc ccggcgtgtc
      241 aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt tataggtgta
      301 aaccttaaac tgccgtacgt ataggctgcg caactgttgg gaagggcgat cggtgcgggc
      361 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      421 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgtaatacga
      481 ctcactatag ggcgaattgg gccctctaga tgcatgctcg agcggccgcc agtgtgatgg
      541 atatctgcag aattcaggca cttcgtggtc tcacattttc aatcttagtt cttagagttc
      601 ctttttgata caacagtagt gatcaagaac tcttcaattc tctgaatacg gagattatat
      661 gggtgacaag attggttctt gttcgtggta tgagtcatca agaaaggctt aagagctcct
      721 taaaaaccct ctcgaactag tgtgcgtgtg gtaagaagac gaaagagccc ttgggcgctc
      781 ttttgatgaa caatgattaa gacttttttg gtagatgtat gacacaaaac aaaacatctc
      841 ccaaatcatt gtcgggtgga aagcaaagtg gcagtacgca cgaccgggat ctatggattt
      901 tctttataat agtggtgaga gtgtgtgtat tagacaaaag aaagtaaaaa aagaaaccac
      961 aaatttctgc aaacttgttc acatgaagac agagacaaag gaatgaagca aaagccagcc
     1021 aatgtttcac ttactcatag actttttgat gtgataagga agccaatagt cttcttccca
     1081 aatcaattca aggaaaagta aaaggacaac attaataatg ttacattatt taacgtgaaa
     1141 gcagtataga ataaagttat ataacttttc ttggcatacc cataagaata attgttctac
     1201 acattctttg aacaccaaat tacatatcta taaatcacaa atattgctat acgttggaga
     1261 tctgaacaaa taacaatgtc catgtggtaa accatttttt tttaggcatc tctacaaaag
     1321 taaagtttta aaattatgta tttttcacta taaaatttag gtgtacgcaa agattattca
     1381 tgctcctgag accacagagt gcctgaattc cagcacactg gcggccgtta ctagtggatc
     1441 cgagctcggt accaagcttg atgcatagct tgagtattct aacgcgtcac ctaaatagct
     1501 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     1561 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     1621 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     1681 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     1741 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1801 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1861 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1921 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1981 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2041 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2101 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2161 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2221 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2281 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2341 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2401 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2461 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2521 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2581 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttagcacgt
     2641 gtcagtcctg ctcctcggcc acgaagtgca cgcagttgcc ggccgggtcg cgcagggcga
     2701 actcccgccc ccacggctgc tcgccgatct cggtcatggc cggcccggag gcgtcccgga
     2761 agttcgtgga cacgacctcc gaccactcgg cgtacagctc gtccaggccg cgcacccaca
     2821 cccaggccag ggtgttgtcc ggcaccacct ggtcctggac cgcgctgatg aacagggtca
     2881 cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc
     2941 ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcac
     3001 tggtcaactt ggccatggtg gccctcctca cgtgctatta ttgaagcatt tatcagggtt
     3061 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3121 cgcgcacatt tccccgaaaa gtgccacctg atgcggtgtg aaataccgca cagatgcgta
     3181 aggagaaaat accgcatcag gaaattgtaa gcgttaataa ttcagaagaa ctcgtcaaga
     3241 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
     3301 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     3361 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     3421 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
     3481 ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     3541 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     3601 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     3661 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     3721 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     3781 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt
     3841 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     3901 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     3961 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga
     4021 aacgatcctc atcctgtctc ttgatcagag cttgatcccc tgcgccatca gatccttggc
     4081 ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca
     4141 gctggcaatt ccggttcgct tgctgtccat aaaaccgccc agtctagcta tcgccatgta
     4201 agcccactgc aagctacctg ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc
     4261 cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt ctacgtgaaa
     4321 aggatctagg tgaagatcct ttttgataat ctcat
//
