LOCUS       sequence-386479-         574 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Component of the Bxb1-rcCre binary site-specific recombinase system,
            expression of the site-specific recombinase Bxb1 (mouse 
            codon-optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Hermann M, Stillhard P, Wildner H, Seruggia D, Kapp V, 
            Sanchez-Iranzo H, Mercader N, Montoliu L, Zeilhofer HU, Pelczar P
  TITLE     Binary recombinase systems for high-resolution conditional 
            mutagenesis.
  JOURNAL   Nucleic Acids Res. 2014 Jan 9.
  PUBMED    24413561
REFERENCE   2  (bases 1 to 574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jan 9."
COMMENT     Sequence Label: pCAG-NLS-HA-Bxb1
FEATURES             Location/Qualifiers
     source          1..574
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     245..267
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     259..276
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     260..276
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    348..572
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
       61 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc
      121 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
      181 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
      241 ttttcccagt cacgacgttg taaaacgacg gccagtggcg gccgctctag cctcgaggct
      301 agaactagtg gatctcgagc cccagctggt tctttccgcc tcagaagcca tagagcccac
      361 cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc cccttgctgt cctgccccac
      421 cccacccccc agaatagaat gacacctact cagacaatgc gatgcaattt cctcatttta
      481 ttaggaaagg acagtgggag tggcaccttc cagggtcaag gaaggcacgg gggaggggca
      541 aacaacagat ggctggcaac tagaaggcac agtc
//
