LOCUS       sequence-386495-       10027 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3020
FEATURES             Location/Qualifiers
     source          1..10027
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1766..1976
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1788..1984
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1835..1970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2016..2618
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2016..2035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2512..2532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2661..2778
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2916..4094
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3147..4085
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4042..4062
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4114..4138
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4139..4148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5696..5720)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5728..5769
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5880..6468
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5933..5953)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6351..6362)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6540..6773
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6851..6972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6888..6907)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6942..6961
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7012..7147
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7167..7186)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7168..7186)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7196..7213)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7196..7212)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7205..7227)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7354..7809
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7441..7460)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7651..7672
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7835..7939
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7940..8008,8009..8800)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8158..8177)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8971..9559
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9460..9479
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9713..9730
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9847..9868
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9883..9888,9889..9906,9907..9913)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9921..9937
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9926..9948
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9945..9961
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9945..9961
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9980..10000
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9982..10000
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag
     1741 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta
     1801 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1861 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1921 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1981 caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc
     2041 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     2101 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     2161 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     2221 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     2281 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     2341 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     2401 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     2461 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     2521 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     2581 gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa
     2641 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2701 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2761 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
     2821 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
     2881 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
     2941 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     3001 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     3061 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     3121 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     3181 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     3241 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     3301 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     3361 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     3421 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     3481 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     3541 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     3601 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     3661 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     3721 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     3781 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     3841 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     3901 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     3961 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     4021 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     4081 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agttggcacc
     4141 atggcggccc ttcccggcac ggtaccgaga atgatgcggc cggctccggg gcagaactac
     4201 ccccgcacgg gattcccttt ggaagtgtcc accccgcttg gccaaggccg ggtcaatcag
     4261 ctgggagggg tcttcatcaa tgggcgaccc ctgcctaacc acatccgcca caagatagtg
     4321 gagatggccc accatggcat ccggccctgt gtcatctccc gacagctgcg tgtctcccac
     4381 ggctgcgtct ccaagattct ttgccgctac caggagaccg ggtccatccg gcctggggcc
     4441 atcggcggca gcaagcccag acaggtggcg actccggatg tagagaaaaa gattgaggag
     4501 tacaagaggg aaaacccagg catgttcagc tgggagatcc gggacaggct gctgaaggat
     4561 gggcactgtg accgaagcac tgtgccctca gtgagttcga ttagccgcgt gctcagaatc
     4621 aagttcggga agaaagagga ggaggatgaa gcggacaaga aggaggacga cggcgaaaag
     4681 aaggccaaac acagcatcga cggcatcctg ggcgacaaag ggaaccggct ggacgagggc
     4741 tcggatgtgg agtcggaacc tgacctccca ctgaagcgca agcagcgacg cagtcggacc
     4801 acattcacgg ccgagcagct ggaggagctg gagaaggcct ttgagaggac ccactaccca
     4861 gacatataca cccgcgagga gctggcgcag aggaccaagc tgacagaggc gcgtgtgcag
     4921 gtctggttca gtaaccgccg cgcccgttgg cgtaagcagg caggagccaa ccagctggcg
     4981 gcgttcaacc accttctgcc aggaggcttc ccgcccaccg gcatgcccac gctgcccccc
     5041 taccagctgc cggactccac ctaccccacc accaccatct cccaagatgg gggcagcact
     5101 gtgcaccggc ctcagcccct gccaccgtcc accatgcacc agggcgggct ggctgcagcg
     5161 gctgcagccg ccgacaccag ctctgcctac ggagcccgcc acagcttctc cagctactct
     5221 gacagcttca tgaatccggc ggcgccctcc aaccacatga acccggtcag caacggcctg
     5281 tctcctcagg tgatgagcat cttgggcaac cccagtgcgg tgcccccgca gccacaggct
     5341 gacttctcca tctccccgct gcatggcggc ctggactcgg ccacctccat ctcagccagc
     5401 tgcagccagc gggccgactc catcaagcca ggagacagcc tgcccacctc ccaggcctac
     5461 tgcccaccca cctacagcac caccggctac agcgtggacc ccgtggccgg ctatcagtac
     5521 ggccagtacg gccagagtga gtgcctggtg ccctgggcgt cccccgtccc cattccttct
     5581 cccaccccca gggcctcctg cttgtttatg gagagctaca aggtggtgtc agggtgggga
     5641 atgtccattt cacagatgga aaaattgaag tccagccaga tggaacagtt caccttgcca
     5701 actttcttgt acaaagtggt tgatatcggt aagcctatcc ctaaccctct cctcggtctc
     5761 gattctacgt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     5821 atatcttgtg gaaaggacga ttgatgccct actataaccc ttacacgcgt taagtcgaca
     5881 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5941 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6001 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6061 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6121 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6181 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6241 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     6301 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6361 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6421 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag
     6481 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     6541 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     6601 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6661 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6721 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta
     6781 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     6841 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6901 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6961 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     7021 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7081 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7141 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     7201 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     7261 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     7321 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     7381 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     7441 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     7501 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     7561 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7621 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7681 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     7741 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     7801 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7861 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7921 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7981 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8041 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8101 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8161 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     8221 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8281 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8341 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8401 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8461 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8521 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8581 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8641 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8701 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8761 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8821 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8881 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8941 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9001 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9061 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     9121 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9181 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9241 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9301 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9361 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9421 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9481 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9541 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9601 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9661 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9721 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     9781 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     9841 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     9901 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     9961 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
    10021 agcttaa
//
