LOCUS       sequence-386563-        8510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LV construct encoding UAS-DsRed-Express2 reporter gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Sloas DC, Tran JC, Marzilli AM, Ngo JT
  TITLE     Tension-tuned receptors for synthetic mechanotransduction and 
            intercellular force detection.
  JOURNAL   Nat Biotechnol. 2023 Jan 16. doi: 10.1038/s41587-022-01638-y.
  PUBMED    36646932
REFERENCE   2  (bases 1 to 8510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 16. doi: 10.1038/s41587-022-01638-y."
COMMENT     Sequence Label: LV UAS-DsRed-Express2-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..8510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..627
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(92..112)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(510..521)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             714..947
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1019..1140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1056..1075)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1110..1129
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1180..1315
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1335..1354)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1336..1354)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1364..1381)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1364..1380)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1373..1395)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1522..1977
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1609..1628)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1819..1840
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2003..2107
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2108..2176,2177..2968)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2326..2345)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3139..3727
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3628..3647
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3881..3898
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4015..4036
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4051..4056,4057..4074,4075..4081)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4089..4105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4094..4116
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4113..4129
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4113..4129
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4148..4168
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4150..4168
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4196..4422
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4423..4603
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4650..4775
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5268..5501
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5686..5730
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5997..6114
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     6328..6352
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6459..6512
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6513..7187
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(6693..6713)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     6911..6930
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7105..7128
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        7255..7765
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7684..7702
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7836..7889
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7890..8489
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7890..7909)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8386..8406
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 ctagcttctg cagcacgtgt ctagaaacgc tggtcgacaa tcaacctctg gattacaaaa
       61 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
      121 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
      181 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
      241 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
      301 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
      361 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
      421 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
      481 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
      541 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
      601 ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat
      661 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
      721 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
      781 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      841 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      901 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
      961 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     1021 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     1081 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     1141 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     1201 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     1261 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     1321 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     1381 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     1441 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     1501 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     1561 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     1621 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     1681 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     1741 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     1801 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     1861 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     1921 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     1981 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     2041 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     2101 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     2161 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     2221 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     2281 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     2341 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     2401 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     2461 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     2521 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     2581 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     2641 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     2701 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     2761 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2821 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2881 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2941 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     3001 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     3061 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     3121 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3181 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3241 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3301 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3361 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3421 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3481 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3541 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3601 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3661 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3721 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3781 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3841 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     3901 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     3961 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     4021 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     4081 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     4141 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     4201 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     4261 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     4321 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     4381 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     4441 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     4501 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     4561 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     4621 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     4681 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     4741 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     4801 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     4861 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     4921 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     4981 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     5041 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     5101 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     5161 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     5221 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     5281 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     5341 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     5401 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     5461 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     5521 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     5581 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     5641 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     5701 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     5761 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     5821 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     5881 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     5941 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt
     6001 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     6061 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttatcgat
     6121 cacgagacta gcctcgagat cgataagctt gatatcgaat tcggagcact gtcctccgaa
     6181 cgtcggagca ctgtcctccg aacgtcggag cactgtcctc cgaacgtcgg agcactgtcc
     6241 tccgaacgga gcatgtcctc cgaacgtcgg agcactgtcc tccgaacgac tagttaggcg
     6301 tgtacggtgg gaggcctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     6361 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctctcgac
     6421 attcgttgga tccttgactt acgcgtttgg ttatgcatga gggcagagga agtcttctaa
     6481 catgcggtga cgtggaggag aatcccggcc ctatggatag cactgagaac gtcatcaagc
     6541 ccttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     6601 agggcgaggg cgagggcaag ccctacgagg gcacccagac cgccaagctg caggtgacca
     6661 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttccag tacggctcca
     6721 aggtgtacgt gaagcacccc gccgacatcc ccgactacaa gaagctgtcc ttccccgagg
     6781 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     6841 actcctccct gcaggacggc accttcatct accacgtgaa gttcatcggc gtgaacttcc
     6901 cctccgacgg ccccgtaatg cagaagaaga ctctgggctg ggagccctcc accgagcgcc
     6961 tgtacccccg cgacggcgtg ctgaagggcg agatccacaa ggcgctgaag ctgaagggcg
     7021 gcggccacta cctggtggag ttcaagtcaa tctacatggc caagaagccc gtgaagctgc
     7081 ccggctacta ctacgtggac tccaagctgg acatcacctc ccacaacgag gactacaccg
     7141 tggtggagca gtacgagcgc gccgaggccc gccaccacct gttccagact cacggtatgg
     7201 acgaattgta caagcacgac gaattgtaag ctagcgcggc cgacgaagtc gcacggggtt
     7261 ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg
     7321 tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg
     7381 cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc
     7441 gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc
     7501 cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg
     7561 accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg
     7621 ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc
     7681 gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga
     7741 ccgaatcacc gacctctctc cccagggggg aatctgcaga ttcggagcca ccggttatta
     7801 cgatatctga gaacatcgat atgggtggtg gtagtgaagg gcggggatcc cttttgactt
     7861 gtggtgatgt ggaggagaac ccaggcccaa tgaccgagta caagcccacg gtgcgcctcg
     7921 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     7981 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     8041 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     8101 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     8161 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     8221 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     8281 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     8341 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     8401 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     8461 ggtgcatgac ccgcaagccc ggtgcctgac cagcacactg gcggccgtta
//
