LOCUS       sequence-386581-        9565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Destination donor vector for AAVS1 targeting (puromycin 
            selection).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, 
            Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
  TITLE     Engineering the AAVS1 locus for consistent and scalable transgene 
            expression in human iPSCs and their differentiated derivatives.
  JOURNAL   Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012.
  PUBMED    26707206
REFERENCE   2  (bases 1 to 9565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012."
COMMENT     Sequence Label: pAAVS1-P-CAG-DEST
FEATURES             Location/Qualifiers
     source          1..9565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1770..1894
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1919..1924,1925..1942,1943..1949)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2003..2662
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2069..2088)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3004..3309
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3188..3207
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3350..3474)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3570..3589)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3635..3690
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3689..3708)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    4246..4775
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(4921..4940)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4922..4940)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4947..4964)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4947..4963)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4956..4978)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5105..5560
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5192..5211)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5402..5423
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5586..5690
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5691..5759,5760..6551)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5909..5928)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6722..7310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7211..7230
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7464..7481
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7598..7619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7634..7639,7640..7657,7658..7664)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7672..7688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7677..7699
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7696..7712
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7696..7712
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7733..7751
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    7764..8567
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    8574..8599
                     /label=SA
                     /note="splice acceptor site"
     CDS             8623..8676
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8686..9285
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8686..8705)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9182..9202
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(9317..9334)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9323..9547
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc gcggccgcgg
     1741 taccggcgcg ccggatcctg caggttatca caagtttgta caaaaaagct gaacgagaaa
     1801 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1861 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     1921 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     1981 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     2041 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     2101 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     2161 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     2221 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     2281 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     2341 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     2401 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     2461 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     2521 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     2581 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     2641 tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta
     2701 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     2761 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     2821 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     2881 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     2941 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     3001 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     3061 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     3121 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     3181 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     3241 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     3301 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     3361 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     3421 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgataac
     3481 tctagagaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
     3541 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
     3601 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     3661 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     3721 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     3781 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     3841 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     3901 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     3961 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     4021 gacctgcagc ccaagcttgg atccctcgag ttaattaaat ctagaagtcg acagtactaa
     4081 gctttgacag aaaagcccca tccttaggcc tcctccttcc tagtctcctg atattgggtc
     4141 taacccccac ctcctgttag gcagattcct tatctggtga cacaccccca tttcctggag
     4201 ccatctctct ccttgccaga acctctaagg tttgcttacg atggagccag agaggatcct
     4261 gggagggaga gcttggcagg gggtgggagg gaaggggggg atgcgtgacc tgcccggttc
     4321 tcagtggcca ccctgcgcta ccctctccca gaacctgagc tgctctgacg cggctgtctg
     4381 gtgcgtttca ctgatcctgg tgctgcagct tccttacact tcccaagagg agaagcagtt
     4441 tggaaaaaca aaatcagaat aagttggtcc tgagttctaa ctttggctct tcacctttct
     4501 agtccccaat ttatattgtt cctccgtgcg tcagttttac ctgtgagata aggccagtag
     4561 ccagccccgt cctggcaggg ctgtggtgag gaggggggtg tccgtgtgga aaactccctt
     4621 tgtgagaatg gtgcgtccta ggtgttcacc aggtcgtggc cgcctctact ccctttctct
     4681 ttctccatcc ttctttcctt aaagagtccc cagtgctatc tgggacatat tcctccgccc
     4741 agagcagggt cccgcttccc taaggccctg ctctgggctt ctgggtttga gtccttggca
     4801 agcccaggag aggcgctcag gcttccctgt cccccttcct cgtccaccat ctcatgcccc
     4861 tggctctcct gccccttccc tacaggggtt cctggctctg ctctagcgat cgccaattcg
     4921 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
     4981 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     5041 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     5101 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5161 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5221 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5281 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5341 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5401 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5461 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5521 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     5581 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5641 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5701 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5761 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5821 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5881 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5941 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     6001 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     6061 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     6121 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     6181 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     6241 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     6301 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     6361 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     6421 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     6481 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6541 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     6601 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6661 cttaacgtga gttttcgttc cactgagcgt caaaccccgt agaaaagatc aaaggatctt
     6721 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6781 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6841 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6901 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6961 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7021 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7081 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7141 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7201 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7261 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7321 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     7381 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     7441 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     7501 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     7561 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     7621 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     7681 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc gaaattaacc
     7741 ctcactaaag ggaacaaaag ctgtgctttc tctgaccagc attctctccc ctgggcctgt
     7801 gccgctttct gtctgcagct tgtggcctgg gtcacctcta cggctggccc agatccttcc
     7861 ctgccgcctc cttcaggttc cgtcttcctc cactccctct tccccttgct ctctgctgtg
     7921 ttgctgccca aggatgctct ttccggagca cttccttctc ggcgctgcac cacgtgatgt
     7981 cctctgagcg gatcctcccc gtgtctgggt cctctccggg catctctcct ccctcaccca
     8041 accccatgcc gtcttcactc gctgggttcc cttttccttc tccttctggg gcctgtgcca
     8101 tctctcgttt cttaggatgg ccttctccga cggatgtctc ccttgcgtcc cgcctcccct
     8161 tcttgtaggc ctgcatcatc accgtttttc tggacaaccc caaagtaccc cgtctccctg
     8221 gctttagcca cctctccatc ctcttgcttt ctttgcctgg acaccccgtt ctcctgtgga
     8281 ttcgggtcac ctctcactcc tttcatttgg gcagctcccc tacccccctt acctctctag
     8341 tctgtgctag ctcttccagc cccctgtcat ggcatcttcc aggggtccga gagctcagct
     8401 agtcttcttc ctccaacccg ggcccctatg tccacttcag gacagcatgt ttgctgcctc
     8461 cagggatcct gtgtccccga gctgggacca ccttatattc ccagggccgg ttaatgtggc
     8521 tctggttctg ggtactttta tctgtcccct ccaccccaca gtggggcaag cttctgacct
     8581 cttctcttcc tcccacaggg cctcgagaga tctggcagcg gagagggcag aggaagtctt
     8641 ctaacatgcg gtgacgtgga ggagaatccc ggccctaggc tcgagatgac cgagtacaag
     8701 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     8761 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     8821 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     8881 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     8941 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     9001 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     9061 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     9121 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     9181 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     9241 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgatctag agggcccgtt
     9301 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     9361 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     9421 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     9481 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     9541 tctatggact agcggtggcg gcctc
//
