LOCUS       sequence-386592-        1721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mutated ubiquitination site (ubiquitination active).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Hoff J, Xiong L, Kammann T, Neugebauer S, Micheel JM, Gassler N, 
            Bauer M, Press AT
  TITLE     RIPK3 promoter hypermethylation in hepatocytes protects from bile 
            acid-induced inflammation and necroptosis.
  JOURNAL   Cell Death Dis. 2023 Apr 18;14(4):275. doi: 
            10.1038/s41419-023-05794-0.
  PUBMED    37072399
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Dis."; date: "2023-04-18"; volume: "14(4):275. doi"; pages: " 
            10.1038/s41419-023-05794-0"
COMMENT     Sequence Label: pcDNA3-RIPK3[K5R]-FLAG
FEATURES             Location/Qualifiers
     source          1..1721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        19..117
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     68..88
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     162..181
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        162..180
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      197..206
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             206..229
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             1291..1299
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gcgtaggatg ttttggcaca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
       61 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
      121 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
      181 gagacccaag cttgccgcca ccatggatta caaggatgac gacgataaga gcgcgggcgg
      241 atccgaattc tgcagatatc catcacactg gcggccgctc gagatgtcgt gcgtcagatt
      301 atggcccagc ggtgcccccg cccccttggt gtccatcgag gaactggaga accaggagct
      361 cgtcggcaaa ggcgggttcg gcacagtgtt ccgggcgcaa cataggaagt ggggctacga
      421 tgtggcggtc aagatcgtaa actcgaaggc gatatccagg gaggtcaagg ccatggcaag
      481 tctggataac gaattcgtgc tgcgcctaga aggggttatc gagaaggtga actgggacca
      541 agatcccaag ccggctctgg tgactaaatt catggagaac ggctccttgt cggggctgct
      601 gcagtcccag tgccctcggc cctggccgct cctttgccgc ctgctgaaag aagtggtgct
      661 tgggatgttt tacctgcacg accagaaccc ggtgctcctg caccgggacc tcaagccatc
      721 caacgtcctg ctggacccag agctgcacgt caagctggca gattttggcc tgtccacatt
      781 tcagggaggc tcacagtcag ggacagggtc cggggagcca gggggcaccc tgggctactt
      841 ggccccagaa ctgtttgtta acgtaaaccg gaaggcctcc acagccagtg acgtctacag
      901 cttcgggatc ctaatgtggg cagtgcttgc tggaagagaa gttgagttgc caaccgaacc
      961 atcactcgtg tacgaagcag tgtgcaacag gcagaaccgg ccttcattgg ctgagctgcc
     1021 ccaagccggg cctgagactc ccggcttaga aggactgaag gagctaatgc agctctgctg
     1081 gagcagtgag cccaaggaca gaccctcctt ccaggatgcc taccaaaaac tgatgagtct
     1141 tccagatggt ggagaacaat atgaatgctg ctgtctccac ggtaaaggat tcctgtctca
     1201 ctcagagcag caataggaga tttctatccc aaatccggcc aggagggaca gaatgggatg
     1261 gtttaggaga acatagaaac cacccttccc taaggaggtc tgggttttga ggggtaacaa
     1321 acgaaattaa agaacccccc ccttttctaa atgccccgcc ttccaaagag gggcgaaagg
     1381 gagagtttcc gccggcacgg gccttttttt tttagggccc ccccactcca aagacaaatt
     1441 ttaaaaaaga cccccccctc tgaaaaaaat tgggcccggg gaaggggggg aaaaagagag
     1501 attgcgcccg ccacaccaca gggccccctt taaaatcggg gggtgaaaaa aaaaaaaaca
     1561 ccccgggggc tcgggggggg ccccccccaa aaaaaaaaaa gagaaaaaga atataaaacc
     1621 ccctctcccc ccccccacaa atttgggggg gggggggccg gccctcctcc ttttctcttt
     1681 tctctctctg tgtcatctct gcctcttgac agcgagaaag a
//
