LOCUS       sequence-386593-        1750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mutated ubiquitination site (ubiquitination inhibited).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Hoff J, Xiong L, Kammann T, Neugebauer S, Micheel JM, Gassler N, 
            Bauer M, Press AT
  TITLE     RIPK3 promoter hypermethylation in hepatocytes protects from bile 
            acid-induced inflammation and necroptosis.
  JOURNAL   Cell Death Dis. 2023 Apr 18;14(4):275. doi: 
            10.1038/s41419-023-05794-0.
  PUBMED    37072399
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Dis."; date: "2023-04-18"; volume: "14(4):275. doi"; pages: " 
            10.1038/s41419-023-05794-0"
COMMENT     Sequence Label: pcDNA3-RIPK3[K5A]-FLAG
FEATURES             Location/Qualifiers
     source          1..1750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        20..118
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     69..89
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     163..182
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        163..181
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      198..207
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             207..230
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agggtggcac tgcttggcac aaaatcaacg ggactttcca aaatgtcgta acaactccgc
       61 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
      121 ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag
      181 ggagacccaa gcttgccgcc accatggatt acaaggatga cgacgataag agcgcgggcg
      241 gatccgaatt ctgcagatat ccatcacact ggcggccgct cgagatgtcg tgcgtcgcct
      301 tatggcccag cggtgccccc gcccccttgg tgtccatcga ggaactggag aaccaggagc
      361 tcgtcggcaa aggcgggttc ggcacagtgt tccgggcgca acataggaag tggggctacg
      421 atgtggcggt caagatcgta aactcgaagg cgatatccag ggaggtcaag gccatggcaa
      481 gtctggataa cgaattcgtg ctgcgcctag aaggggttat cgagaaggtg aactgggacc
      541 aagatcccaa gccggctctg gtgactaaat tcatggagaa cggctccttg tcggggctgc
      601 tgcagtccca gtgccctcgg ccctggccgc tcctttgccg cctgctgaaa gaagtggtgc
      661 ttgggatgtt ttacctgcac gaccagaacc cggtgctcct gcaccgggac ctcaagccat
      721 ccaacgtcct gctggaccca gagctgcacg tcaagctggc agattttggc ctgtccacat
      781 ttcagggagg ctcacagtca gggacagggt ccggggagcc agggggcacc ctgggctact
      841 tggccccaga actgtttgtt aacgtaaacc ggaaggcctc cacagccagt gacgtctaca
      901 gcttcgggat cctaatgtgg gcagtgcttg ctggaagaga agttgagttg ccaaccgaac
      961 catcactcgt gtacgaagca gtgtgcaaca ggcagaaccg gccttcattg gctgagctgc
     1021 cccaagccgg gcctgagact cccggcttag aaggactgaa ggagctaatg cagctctgct
     1081 ggagcagtga gcccaaggac agaccctcct tccagaatgc ctaccaaaaa ctgatgagtc
     1141 ttcccaatgg tggagaaaaa tatgaatgct gctgtctcca cggtaaagga tttctgtctc
     1201 actcagacca ccaataggag atttttatcc caagtcaggc caggagggga agaaatggat
     1261 gggtttttga gaaacaaaaa aaacaccccc cctaaagatg tttgggtttt tgaggggtaa
     1321 caaaacgaat taaagaactc cccccttgtt ttaaaaagcc caccctttca aaagaagggg
     1381 ggcaaaaaga aggggtcccc ccctgggggg gggctctttt attttgtggg cccccccacc
     1441 cccacaaccc ttttttaaaa aaaaccgccc tcccccctcg caaccccccg ccgccccggg
     1501 ggggggtgga aaagagaagg gtttgccccc cccccacaca agcggcgccc ttttattatg
     1561 tggggggaaa aaaaaaaaaa acaaccgccg cgcggctgct gggggggccc cccccccaca
     1621 caaaacaaaa ccgggttata agatttatat acccttctcc cctccccctc cccacccata
     1681 tttttggggg agaggggaga gagggctctc tccctctctt cccccgccac caaccccaca
     1741 cgagcagaat
//
