LOCUS       sequence-386694-       12341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pInducer21 plasmid containing the human MEFV gene with a deletion of
            residues 88 - 310 (the resulting pyrin protein lacks the 
            Phosphorylated Linker Domain domain) with a 3xFlag tag at the 
            N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12341)
  AUTHORS   Chirita D, Bronnec P, Magnotti F, Dalmon S, Martin A, Popoff M, 
            Gerfaud-Valentin M, Seve P, Belot A, Contis A, Duquesne A, Nocturne 
            G, Lemelle I, Georgin-Lavialle S, Boursier G, Touitou I, Jamilloux 
            Y, Henry T
  TITLE     Mutations in the B30.2 and the central helical scaffold domains of 
            pyrin differentially affect inflammasome activation.
  JOURNAL   Cell Death Dis. 2023 Mar 25;14(3):213. doi: 
            10.1038/s41419-023-05745-9.
  PUBMED    36966139
REFERENCE   2  (bases 1 to 12341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Dis."; date: "2023-03-25"; volume: "14(3):213. doi"; pages: " 
            10.1038/s41419-023-05745-9"
COMMENT     Sequence Label: pInducer 21-3xFlag-hMEFV-Delta-PLD
FEATURES             Location/Qualifiers
     source          1..12341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2768..2833
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(4570..4594)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4647..4673
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4893..6071
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5124..6062
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6019..6039
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    6141..6174
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(6181..6798,6799..6804,6805..6840,6841..6843,6844..6879,
                     6880..6882,6883..6918,6919..6927)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6980..7553
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7147..7164)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7374..7393
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7569..7571,7572..7574,7575..8288)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7569..7571,7572..7574,7575..8285)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7614..7635)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7875..7894)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8222..8243
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8424..8443
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8731..9319
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8784..8804)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9202..9213)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9394..9627
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9746..9764)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9832..9936
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9937..10005,10006..10797)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10155..10174)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10968..11556
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11457..11476
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11710..11727
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11967..11988
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12003..12008,12009..12026,12027..12033)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12041..12057
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12046..12068
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12065..12081
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12065..12081
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12131..12150
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt cagtcgactg gatccggtac
     2761 caccatggac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga
     2821 tgacgatgac aagcttgcgg ccgcggctaa gacccctagt gaccatctgc tgtccaccct
     2881 ggaggagctg gtgccctatg acttcgagaa gttcaagttc aagctgcaga acaccagtgt
     2941 gcagaaggag cactccagga tcccccggag ccagatccag agagccaggc cggtgaagat
     3001 ggccactctg ctggtcacct actatgggga agagtacgcc gtgcagctca ccctgcaggt
     3061 cctgcgggcc atcaaccagc gcctgctggc cgaggagctc cacagggcag ccattcaggg
     3121 aaggccacca gacacggctg cgagtccccg ctgccacgcc caggaaggag acccagttga
     3181 cggtacctgt gtgcgtgatt cctgcagctt ccccgaggca gtttctgggc acccccaggc
     3241 ctcaggcagc cgctcacctg gctgcccccg gtgccaggac tcccatgaaa ggaagagccc
     3301 gggaagccta agcccccagc ccctgccaca gtgtaagcgc cacctgaagc aggtccagct
     3361 gctcttctgt gaggatcacg atgagcccat ctgcctcatc tgcagtctga gtcaggagca
     3421 ccaaggccac cgggtgcgcc ccattgagga ggtcgccctg gaacacaaga agaaaattca
     3481 gaagcagctg gagcatctga agaagctgag aaaatcaggg gaggagcagc gatcctatgg
     3541 ggaggagaag gcagtgagct ttctgaaaca aactgaagcg ctgaagcagc gggtgcagag
     3601 gaagctggag caggtgtact acttcctgga gcagcaagag catttctttg tggcctcact
     3661 ggaggacgtg ggccagatgg ttgggcagat caggaaggca tatgacaccc gcgtatccca
     3721 ggacatcgcc ctgctcgatg cgctgattgg ggaactggag gccaaggagt gccagtcaga
     3781 atgggaactt ctgcaggaca ttggagacat cttgcacagg gctaagacag tgcctgtccc
     3841 tgaaaagtgg accactcctc aagagataaa acaaaagatc caactcctcc accagaagtc
     3901 agagtttgtg gagaagagca caaagtactt ctcagaaacc ctgcgttcag aaatggaaat
     3961 gttcaatgtt ccagagctga ttggcgctca ggcacatgct gttaatgtga ttctggatgc
     4021 agaaaccgct taccccaacc tcatcttctc tgatgatctg aagagtgtta gacttggaaa
     4081 caagtgggag aggctgcctg atggcccgca aagatttgac agctgtatca ttgttctggg
     4141 ctctccgagt ttcctctctg gccgccgtta ctgggaggtg gaggttggag acaagacagc
     4201 atggatcctg ggagcctgca agacatccat aagcaggaaa gggaacatga ctctgtcgcc
     4261 agagaatggc tactgggtgg tgataatgat gaaggaaaat gagtaccagg cgtccagcgt
     4321 tcccccgacc cgcctgctaa taaaggagcc tcccaagcgt gtgggcatct tcgtggacta
     4381 cagagttgga agcatctcct tttacaatgt gacagccaga tcccacatct atacattcgc
     4441 cagctgctct ttctctgggc cccttcaacc tatcttcagc cctgggacac gtgatggagg
     4501 gaagaacaca gctcctctga ctatctgtcc agtgggtggt caggggcctg actgactcga
     4561 gatatctaga cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct
     4621 cgagtctaga gggcccgcgg ttcgaatacc catacgacgt cccagactac gcttagtaat
     4681 gattaattaa actagaaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     4741 gtaacccacc caagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc
     4801 tcctcacggc gagccgcgta agatctggcc tccgcgccgg gttttggcgc ctcccgcggg
     4861 cgcccccctc ctcacggcga gcctagtcgt gaggctccgg tgcccgtcag tgggcagagc
     4921 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
     4981 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
     5041 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
     5101 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
     5161 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
     5221 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
     5281 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
     5341 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
     5401 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
     5461 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     5521 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     5581 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     5641 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     5701 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     5761 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     5821 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     5881 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     5941 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     6001 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     6061 aggtgtcgtg aagcggccgc catgctagta aattgtccgc taaattctgg ccgtttttgg
     6121 cttttttgtt agacggatcc ataacttcgt ataatgtatg ctatacgaag ttatgccacc
     6181 atgtctagac tggacaagag caaagtcata aacggagctc tggaattact caatggtgtc
     6241 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
     6301 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg
     6361 gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
     6421 aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
     6481 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
     6541 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
     6601 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca
     6661 cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag
     6721 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
     6781 ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc
     6841 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
     6901 tttgaccttg acatgctccc cgggtaacta agtaaggatc cgcggccgca ctagaggaat
     6961 tccgcccctc tccccccccc ccctctccct cccccccccc taacgttact ggccgaagcc
     7021 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     7081 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     7141 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     7201 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     7261 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     7321 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     7381 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     7441 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     7501 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttacc
     7561 ggtccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     7621 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     7681 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     7741 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     7801 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     7861 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     7921 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     7981 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     8041 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     8101 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     8161 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     8221 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     8281 acaagtaagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     8341 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     8401 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     8461 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     8521 cgccttctat cgccttcttg acgagttctt ctgatgtaca agtaaagcgg ccgcgactct
     8581 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     8641 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgtttag tccctcccaa
     8701 ttcgatatca agcttatcgc gatagatcct aatcaacctc tggattacaa aatttgtgaa
     8761 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     8821 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     8881 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     8941 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     9001 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     9061 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     9121 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     9181 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     9241 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     9301 ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag
     9361 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     9421 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     9481 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9541 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9601 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     9661 tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg
     9721 ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     9781 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     9841 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     9901 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     9961 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    10021 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    10081 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    10141 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
    10201 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    10261 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    10321 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    10381 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    10441 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    10501 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    10561 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    10621 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    10681 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    10741 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    10801 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    10861 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    10921 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    10981 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    11041 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    11101 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    11161 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    11221 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    11281 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    11341 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    11401 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    11461 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    11521 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    11581 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    11641 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    11701 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    11761 ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta
    11821 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
    11881 tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg
    11941 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
    12001 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
    12061 cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg
    12121 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    12181 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    12241 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    12301 agaaattgta tttgttaaat atgtactaca aacttagtag t
//
