LOCUS       sequence-386781-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ~100 repeats of the human telomere seed sequence fused to the 
            puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to 
            obtain transfectable construct for chromosomal arm loss induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Girish V, Lakhani AA, Thompson SL, Scaduto CM, Brown LM, Hagenson 
            RA, Sausville EL, Mendelson BE, Kandikuppa PK, Lukow DA, Yuan ML, 
            Stevens EC, Lee SN, Schukken KM, Akalu SM, Vasudevan A, Zou C, 
            Salovska B, Li W, Smith JC, Taylor AM, Martienssen RA, Liu Y, Sun R,
            Sheltzer JM
  TITLE     Oncogene-like addiction to aneuploidy in human cancers.
  JOURNAL   Science. 2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521.
  PUBMED    37410869
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2023 Jul 6:eadg4521. doi: 10.1126/science.adg4521."
COMMENT     Sequence Label: EF1a_Puro_Telo_v2
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..609
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="EYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVT
                     ELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQ
                     QQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAP
                     RNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     506..526
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     621..637
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     622..638
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gap             879..889
                     /estimated_length=11
     gap             958..970
                     /estimated_length=13
ORIGIN
        1 cnnnnnnnnn tgancgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
       61 cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
      121 gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
      181 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
      241 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
      301 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
      361 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
      421 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
      481 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
      541 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
      601 ggtgcctgac gggccccccc tcgaggtcga cggtatcgat aancttgatg gttagggtta
      661 nggttagggt tagggttang gtnnnggtta nggttanggt nnnggtnang gttanggtta
      721 gggtnnnggt tanggttang gttanggnta gggttangnt tnnggttang gtcanggttn
      781 nggcnnnggn nnnngntacg ggcnngntna cggtnnnngt nnngnntacg gcnngggtcc
      841 gnncnngcnn nnnnnncngg ttcnnnnang gtnangttnn nnnnnnnnnc nnnnnnngtn
      901 nngntnnnnt ncggnnnggt nnnnnnngnt angttnngtn nnnannnngn nnnganannn
      961 nnnnnnnnnn annnnactnn nnnnnc
//
