LOCUS       sequence-386788-        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Stable and dox. inducible expression of Trim69 isoform B (codon 
            optimized) after retroviral-mediated gene transfer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Song Y, Nguyen XN, Kumar A, da Silva C, Picard L, Etienne L, 
            Cimarelli A
  TITLE     Trim69 is a microtubule regulator that acts as a pantropic viral 
            inhibitor.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2211467119. doi: 
            10.1073/pnas.2211467119. Epub 2022 Oct 17.
  PUBMED    36251989
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-10-25"; volume: "119(43):e2211467119. 
            doi"; pages: " 10.1073/pnas.2211467119. Epub 2022 Oct 17"
COMMENT     Sequence Label: pRetroX-Tight-Pur-Trim69 isoform B (codon opti...
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggactaca aagacgatga cgataagggt ggcggagcgg ccgccgagga ggagctggcc
       61 attcagcagg gccagctgga gaccaccctg aaggagctgc aaaccctcag gaacatgcag
      121 aaggaggcca tcgccgctca caaggaaaac aagctgcacc tccagcagca cgtgtccatg
      181 gagttcctca agctccatca gttcctgcac tccaaggaaa aggatatcct caccgagctg
      241 agagaggaag gcaaagccct gaatgaagag atggagctga acctgagcca gctccaggag
      301 cagtgcctgc tggccaagga catgctcgtg agcatccagg ccaagaccga acagcagaac
      361 agcttcgact tcctcaagga tattaccacc ctgctgcact ccctggagca gggcatgaag
      421 gtcctggcca ccagagagct catttccagg aagctgaacc tgggccagta taagggcccc
      481 attcagtaca tggtgtggag ggagatgcag gacaccctgt gtcccggcct gagccctctg
      541 acactggacc ccaaaacagc ccaccccaat ctggtgctgt ccaagagcca gacctccgtg
      601 tggcatggcg acatcaagaa gatcatgccc gatgaccccg agaggtttga cagcagcgtc
      661 gctgtgctgg gctccagagg ctttaccagc ggcaaatggt actgggaagt ggaggtggcc
      721 aaaaagacca aatggacagt gggagtggtg agggaaagca ttatcaggaa gggcagctgt
      781 cctctgacac ctgagcaggg cttttggctg ctgaggctga gaaaccagac agacctgaag
      841 gccctggacc tccccagctt cagcctgacc ctgacaaaca acctggacaa ggtcggcatc
      901 tacctggact acgagggagg ccagctgagc ttctataatg ccaaaaccat gacacacatc
      961 tatacctttt ccaacacatt tatggagaag ctgtaccctt atttctgtcc ctgcctgaac
     1021 gacggcggcg aaaataagga gcccctgcac attctgcacc cccagtga
//
