LOCUS       sequence-386789-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Stable and dox. inducible expression of Trim69 isoform C (codon 
            optimized) after retroviral-mediated gene transfer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Song Y, Nguyen XN, Kumar A, da Silva C, Picard L, Etienne L, 
            Cimarelli A
  TITLE     Trim69 is a microtubule regulator that acts as a pantropic viral 
            inhibitor.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2211467119. doi: 
            10.1073/pnas.2211467119. Epub 2022 Oct 17.
  PUBMED    36251989
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-10-25"; volume: "119(43):e2211467119. 
            doi"; pages: " 10.1073/pnas.2211467119. Epub 2022 Oct 17"
COMMENT     Sequence Label: pRetroX-Tight-Pur-Trim69 isoform C (codon opti...
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggactaca aagacgatga cgataagggt ggcggagcgg ccgccgagtt cctcaagctc
       61 catcagttcc tgcactccaa ggaaaaggat atcctcaccg agctgagaga ggaaggcaaa
      121 gccctgaatg aagagatgga gctgaacctg agccagctcc aggagcagtg cctgctggcc
      181 aaggacatgc tcgtgagcat ccaggccaag accgaacagc agaacagctt cgacttcctc
      241 aaggatatta ccaccctgct gcactccctg gagcagggca tgaaggtcct ggccaccaga
      301 gagctcattt ccaggaagct gaacctgggc cagtataagg gccccattca gtacatggtg
      361 tggagggaga tgcaggacac cctgtgtccc ggcctgagcc ctctgacact ggaccccaaa
      421 acagcccacc ccaatctggt gctgtccaag agccagacct ccgtgtggca tggcgacatc
      481 aagaagatca tgcccgatga ccccgagagg tttgacagca gcgtcgctgt gctgggctcc
      541 agaggcttta ccagcggcaa atggtactgg gaagtggagg tggccaaaaa gaccaaatgg
      601 acagtgggag tggtgaggga aagcattatc aggaagggca gctgtcctct gacacctgag
      661 cagggctttt ggctgctgag gctgagaaac cagacagacc tgaaggccct ggacctcccc
      721 agcttcagcc tgaccctgac aaacaacctg gacaaggtcg gcatctacct ggactacgag
      781 ggaggccagc tgagcttcta taatgccaaa accatgacac acatctatac cttttccaac
      841 acatttatgg agaagctgta cccttatttc tgtccctgcc tgaacgacgg cggcgaaaat
      901 aaggagcccc tgcacattct gcacccccag tga
//
