LOCUS       sequence-386790-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Stable and dox. inducible expression of Trim69 isoform D (codon 
            optimized) after retroviral-mediated gene transfer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Song Y, Nguyen XN, Kumar A, da Silva C, Picard L, Etienne L, 
            Cimarelli A
  TITLE     Trim69 is a microtubule regulator that acts as a pantropic viral 
            inhibitor.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Oct 25;119(43):e2211467119. doi: 
            10.1073/pnas.2211467119. Epub 2022 Oct 17.
  PUBMED    36251989
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-10-25"; volume: "119(43):e2211467119. 
            doi"; pages: " 10.1073/pnas.2211467119. Epub 2022 Oct 17"
COMMENT     Sequence Label: pRetroX-Tight-Pur-Trim69 isoform D (codon opti...
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggactaca aagacgatga cgataagggt ggcggagcgg ccgccgagtt cctcaagctc
       61 catcagttcc tgcactccaa ggaaaaggat atcctcaccg agctgagaga ggaaggcaaa
      121 gccctgaatg aagagatgga gctgaacctg agccagctcc aggagcagtg cctgctggcc
      181 aaggacatgc tcgatattac caccctgctg cactccctgg agcagggcat gaaggtcctg
      241 gccaccagag agctcatttc caggaagctg aacctgggcc agtataaggg ccccattcag
      301 tacatggtgt ggagggagat gcaggacacc ctgtgtcccg gcctgagccc tctgacactg
      361 gaccccaaaa cagcccaccc caatctggtg ctgtccaaga gccagacctc cgtgtggcat
      421 ggcgacatca agaagatcat gcccgatgac cccgagaggt ttgacagcag cgtcgctgtg
      481 ctgggctcca gaggctttac cagcggcaaa tggtactggg aagtggaggt ggccaaaaag
      541 accaaatgga cagtgggagt ggtgagggaa agcattatca ggaagggcag ctgtcctctg
      601 acacctgagc agggcttttg gctgctgagg ctgagaaacc agacagacct gaaggccctg
      661 gacctcccca gcttcagcct gaccctgaca aacaacctgg acaaggtcgg catctacctg
      721 gactacgagg gaggccagct gagcttctat aatgccaaaa ccatgacaca catctatacc
      781 ttttccaaca catttatgga gaagctgtac ccttatttct gtccctgcct gaacgacggc
      841 ggcgaaaata aggagcccct gcacattctg cacccccagt ga
//
